The digestibilities of dry matter (DM), crude protein (CP), ether extract (EE), neutral detergent fiber (NDF), gross energy (GE) and total digestible nutrients (TDN) were determined by using four internal markers (acid detergent fiber - ADF and lignin and acid insoluble ash - AIA). The three first markers were submitted to in vitro disappearance for three and six days and the results were compared with data determined by total feces collection. The nutrient digestibility estimated by NDF, ADF and lignin incubated during six days did not differ from that using total feces collection, while the markers incubated during three days and AIA subestimated the digestibility of nutrients, due to their lower recovery.
AIA; ADF; lignin; NDF; total collection of feces
