Two experiments, using 8 to 18 days old Avian -Farm male broiler chicks, were conducted to evaluate the effect of microbial enzymes on the ileal digestibility coefficient (DC) of dry mater (DM), crude protein (CP), energy (E), calcium (Ca) and phosphorus (P) in corn soybean meal diets with different level of Ca and available P (Pa). Chromic oxide (0,5%) was added to the diets, as an indigestible marker, to estimate ileal digestibility. All chicks were killed at the end of the experiments (19 days) to collect the ileal content. The first experiment was a factorial arrangement of 2 x 2, with six replicates (n=240, 10 chicks per unit). Diets were formulated to contain two levels of Ca and Pa normal (1%Ca/0.45%Pa) and low (0.70%Ca/0.32%Pa) x two level of phytase (Phy) enzyme (0 and 1 kg/t of diet). The second experiment was a factorial arrangement of 2 x 3, six replicates (n=360, 10 chicks per unit). Diets were formulated with two levels of Ca and Pa normal (1%Ca/0.45%Pa) and low (0.70%Ca/0.32%Pa) x three combination of a multienzyme complex (MC). The combination were 0 kg/t for the control (C), 2 kg/t (MC) and 2 kg/t of the MC plus 1 kg/t of phy (MC+phy). In the first experiment the diets with Phy had a higher digestibility for DM (5.2%), CP (2.4%) and GE (3,8%). Phytase improve the digestibility of P and Ca in both levels of Ca and Pa. In the second experiment, the addition of the MC improved DC of CP, P and Ca in 3%; 4.7 and 7.8%, respectively. The association of MC + Phy improved Ca and P digestibility. Interaction was observed in the energy digestibility. The addition of the MC improved the digestibility in both Ca and P levels, however the addition of the CM+Phy improved energy DC only in the diets with low Ca and P levels.
amillase; broiler chicks; cellulase; digestibility; protease; phytase
