Abstract
Among the new substances that were synthesised by the chemical industiy during the last decades, with the aim to improve human prosperity, some cause negative effects on non target organisms like fish. Among the pesticides, the organophosphates (OP) are widely used in cattle management, agriculture and aquaculture activities. Brachydanio rerio Hamilton-Buchman, 1822 is used world-wide in fish-tests for the establishment of the lethal levels of pesticides. Nevertheless, these fish show morphological changes in their hepatocytes when exposed experimentally for 24 hours to the sublethal dose of 0.025 µl/l of the OP Dimethoate 500, in acute tests. Right hours after exposure, a high density of cells with cytoplasmic granulation is seen as well as focal necrosis. The main changes that occurred in the first 24 hours of exposure were the loss of the typical polygonal cell shape and of detectable cell limits, lateral migration of nuclei, nuclear size and shape, condensation of chromatin and pycnosis, increased cytoplasmic granulation followed by vacuolisation. After 48 hours in clean water, 72 hours from contamination, besides significant morphological changes and necrosis, focal regeneration of the hepatocytes, canaliculi, ducts and sinusoids were seen. Even having been exposed to the half of the dose considered as safe according to the general pesticide allowance rules, significant changes of the liver tissue were observed.
Dimethoate; histopathology; fish; liver; organophosphates; sublethal contamination
Liver histopathology of the fish brachydanio rerio hamilton-buchman after acute exposure to sublethal levels of the organophosphate dimethoate 500
Edson de Lara RodriguesI; Edith Fanta1 1 Correspondence to: Universidade Federal do Paraná, Departamento de Biologia Celular. Caixa Postal 19031, 81531-970 Curitiba, Paraná, Brazil
IInstituto Adventista de Ensino, Faculdade Adventista de Ciências. Caixa Postal 12630, 04744-970 São Paulo, São Paulo, Brazil
ABSTRACT
Among the new substances that were synthesised by the chemical industiy during the last decades, with the aim to improve human prosperity, some cause negative effects on non target organisms like fish. Among the pesticides, the organophosphates (OP) are widely used in cattle management, agriculture and aquaculture activities. Brachydanio rerio Hamilton-Buchman, 1822 is used world-wide in fish-tests for the establishment of the lethal levels of pesticides. Nevertheless, these fish show morphological changes in their hepatocytes when exposed experimentally for 24 hours to the sublethal dose of 0.025 µl/l of the OP Dimethoate 500, in acute tests. Right hours after exposure, a high density of cells with cytoplasmic granulation is seen as well as focal necrosis. The main changes that occurred in the first 24 hours of exposure were the loss of the typical polygonal cell shape and of detectable cell limits, lateral migration of nuclei, nuclear size and shape, condensation of chromatin and pycnosis, increased cytoplasmic granulation followed by vacuolisation. After 48 hours in clean water, 72 hours from contamination, besides significant morphological changes and necrosis, focal regeneration of the hepatocytes, canaliculi, ducts and sinusoids were seen. Even having been exposed to the half of the dose considered as safe according to the general pesticide allowance rules, significant changes of the liver tissue were observed.
Key words: Dimethoate, histopathology, fish, liver, organophosphates, sublethal contamination
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ACKNOWLEDGEMENTS. The research was conducted with a grant from FUNPAR/UFPR, a CNPq Master Stipend for E.L. Rodrigues, and a CNPq Scientist Stipend for E.Fanta. The authors wish to thank the Biologists A.C.Vianna and S.Romão for the histological photography.
Recebido em 17.IV.1997; aceito em 10.VI.1998.
- Alabaster, J.S. 1969. Survival of fish in 164 herbicides, insecticides, fungicides, wetting agents and miscellaneous substances. International Pest. Control.:29-35.
- Annes, M.A. 1978. Hepatic pathology in a freshwater teleost Channa punctatus (Bloch) exposed to sublethal and chronic levels of three organophosphorus insecticides. Bull. Environ. Contam. Toxicol. 19:525-527.
- Ansari, B.A. & K. Kumar. 1988. Diazinon toxicity: effect on protein and nucleic acid metabolism in the liver of zebrafish, Brachydanio rerio (Cyprinidae). Sci. Total Environ. 76 (1):63-68.
- Ansari, B.A.; R. Srivastava & K. Kumar. 1986. Malathion toxicity: Pathological changes in the ovary of Zebrafish Brachydanio rerio (Cyprinidae). Bol. Fisiol. Anim. 10:95-101.
- Bender, M.E. 1969. Uptake and retention of malathion by the carp. Fish-cult. 31:155-159.
- Brasil, Ministério da Agricultura. 1988. Pesticidas: métodos de análise e informações técnicas. Curitiba, 1, p. 91-93.
- Braunbeck, T.; V. Storch & R. Nagel. 1989. Sex-specific reaction of liver ultrastructure in Zebrafish, Brachydanio rerio after prolonged sublethal exposure to 4-nitrophenol. Aquat. Toxicol. 14 (3):185-202.
- Brzezinski, J. & B. Wysocka-Paruszewska. 1988. Neurochemical alterations in rat brain as a test for studying the neurotoxicity of Organophosphorus Insecticides. Arch. Toxicol. 4:479-481.
- Bunton, T.E.; S.M. Baksi; S.M. George & J.M. Frazier. 1987. Abnormal hepatic copper storage in a Teleost fish (Morone americana). Vet. Pathol. 24 (6):515-525.
- Coppage, D.L. 1972. Organophosphate pesticides: specific level of brain AchE inhibition related to death in sheepshead minnows. Trans. Amer. Fish. Soc. 101:534-540.
- Culling, C.F.A.; R.T. Allison & W.T. Barr. 1985. Cellular pathology technique. London, Butterworth & Co., 642p.
- Eller, L.L. 1971. Histopathological lesions in cutthroat trout Salmo clarki exposed chronically to the insecticide Endrin. Amer. Jour. Pathol. 64:321-330.
- Fanta, E. 1991. Ação de Poluentes sobre tecidos, p. 32-37. In: H.S.L. Santos. (Ed.) Histologia de Peixes. Jaboticabal, FCAV-UNESP, 80p.
-
14______. 1994. Estudos de comportamento de peixes dentro de um contexto multidisciplinar e sua aplicação prática. Anais do Encontro de Etologia, Cananéia, 12:14-30.
- ______. 1997 Organofosforados: bons para a agricultura, ruins para os peixes. Germinis 1 (1):3-4
- Gill, T.S.; J. Pande & H. Tewari. 1990. Sublethal effects of an organophosphorus insecticide on certain metabolite levels in a freshwater fish, Puntius conchonius (Hamilton). Pestic. Biochem. Physiol. 36 (3):290-299.
- Gill, T.S.; J.C. Pant & J. Pant. 1988. Gill, liver and kidney lesions associated with experimental exposures to Carbaryl and Dimethoate in the fish Puntius conchonius (Ham.). Bull. Environ. Contain. Toxicol. 41 (1):71-78.
- Hibya, T. 1982. An Atlas of Fish Histology: Normal and Pathological Features., p.82-90. Tokyo, Kodansha & Gustav Fischer Verlag, 147p.
- Kendall, M.W. 1977. Acute effects of methyl mercury toxicity in channel catfish Ictalurus punctatus liver. Bull. Environ. Contam. Toxicol. 18:143-147.
- Koelle, G.B. & A. Gilman. 1949. Anticholinesterase. Jour. Pharmacol. Exp. Ther. 95:166-216.
- Kumar, K. & B.A. Ansari. 1984. Malathion toxicity: skeletal deformities in zebrafish Brachydanio rerio (Cyprinidae). Pest. Sci. 15:107-111.
- ______. 1986. Malathion toxicity: effect on the liver of the fish Brachydanio rerio (Cyprinidae). Ecotoxicol. Environ. Safety. 12:199-205.
- Laale, H.W. 1977. The biology and use of zebrafish, Brachydanio rerio in fisheries research. Jour. Fish. Biol. 10:121-173.
- Larini, L. 1979. Toxicologia dos Inseticidas. São Paulo, Sarvier, 172p.
- Lingaraja, T. & V.K. Venugopalan. 1978. Pesticides induced physiological and behavioural changes in an estuarine teleost Therapon jarhua (Forsk). Fish Technol. 15:115-119.
- Loomis, T.A. & A.W. Hayes. 1996. Loomis essentials of toxicology. New York Acad. Press, 282p.
- Matsumura, F. 1985. Toxicology of insecticides. New York, Plenum Press, 598p.
- Pickering, Q.H.; C. Henderson & A.E. Lenke. 1962. The toxicity of organophosphorus insecticides to different species of warm water fishes. Trans. Amer. Fish Soc. 91 (2):175-184.
- Randall, D.J.; C.J. Brauner; R.V. Thurston & J.F. Neuman. 1996. Water chemistry at the gill surfaces of fish and the uptake of xenobiotics. In: E.W. Taylor (Ed.) Toxicology of aquatic pollution. Cambridge Univers. Press. Soc. Exptl. Biol. Seminar Ser. 57:1-16.
- Silva, H.C.; H.S.G. Medina; E. Fanta & M. Bacila. 1993. Sub-lethal effects of the organophosphate Folidol 600 (Methyl Parathion) on Callichthys callichthys (Pisces: Teleostei). Comp. Biochem. Physiol. 105 (2):197-201.
- Verma, S.R.; S.K. Bansal & A.K. Gupta. 1982. Bioassay trials with twenty three pesticides to a freshwater teleost Saccobranchus fossilis. Water Res. 16:525-529.
- Weiss, P. 1974. Ultrastructural changes induced by low concentrations of DDT in the livers of the zebrafish and guppy. Chem. Biol. Interact. 8:25-30.
Publication Dates
-
Publication in this collection
10 July 2009 -
Date of issue
1998
History
-
Received
17 Apr 1997 -
Accepted
10 June 1998