Ultrastructure (SEM) of the gills of Prochilodus Scrofa Steindachner (Pisces, Teleostei)

Abstract

Gills arches of the freshwater fish Prochilodus scrofa Steindachner, 1881 (Lt= 8.0-12.4cm) were removed to be analyzed in a scanning electron microscope. The morphology of the superficial structures of the gill filaments and pharyngeal region of the gills arches was discussed and related to their functional aspects. A great quantity of mucous secretory cells and of microridges, along with the pavement cell surface of the primary lamellae and branchial pharyngeal region, indicate the existence of a protection strategy of the respiratory lamellae. The chloride cells are abundant, specially on the distal portion of the primary lamellae, and bring out the osmo regulatory capacity of this species. This fish seems not to be a filtering one according to: its short and simple gill rakers; presence of several taste buds turned towards the mouth opening; evidence of spines on the external side of the arches and an abundant secretion of mucous in the pharyngeal region. This species probably selects the food to be swallowed through its chemical receptors and retains it with the help of spines and mucous secretion this way protecting the gills filaments against the rubbing of particles over them.

Gill morphology; scanning; freshwater fish; Prochilodus scrofa


Ultrastructure (SEM) of the gills of Prochilodus Scrofa Steindachner (Pisces, Teleostei)

Daura Regina Eiras-StofellaI; Patricia Charvet-AlmeidaII

ICentro de Microscopia Eletrônica, Universidade Federal do Paraná. Caixa Postal 19031, 81530-900 Curitiba, Paraná, Brazil

IICaixa Postal 2632,80001-970 Curitiba, Paraná, Brazil

ABSTRACT

Gills arches of the freshwater fish Prochilodus scrofa Steindachner, 1881 (Lt= 8.0-12.4cm) were removed to be analyzed in a scanning electron microscope. The morphology of the superficial structures of the gill filaments and pharyngeal region of the gills arches was discussed and related to their functional aspects. A great quantity of mucous secretory cells and of microridges, along with the pavement cell surface of the primary lamellae and branchial pharyngeal region, indicate the existence of a protection strategy of the respiratory lamellae. The chloride cells are abundant, specially on the distal portion of the primary lamellae, and bring out the osmo regulatory capacity of this species. This fish seems not to be a filtering one according to: its short and simple gill rakers; presence of several taste buds turned towards the mouth opening; evidence of spines on the external side of the arches and an abundant secretion of mucous in the pharyngeal region. This species probably selects the food to be swallowed through its chemical receptors and retains it with the help of spines and mucous secretion this way protecting the gills filaments against the rubbing of particles over them.

Key words: Gill morphology; scanning; freshwater fish; Prochilodus scrofa

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ACKNOWLEDGEMENTS. To Vera Regina Fontana Pionteke for her help with the photographic work in this paper and to the National Research Council of Brazil (CNPq) for the partial financial support.

Recebido em 28.II.1997; aceito em 17.IV.1998.

  • Avella, M.; J. Berhaut; M. Bornancin. 1993. Salinity tolerance of two tropical fishes, Oreochromis aureus and O. niloticus. I. Biochemical and morphological changes in the gill epithelium. Jour. Fish. Biol., London, 42:243-254.
  • Avella, M; J. Berhaut; P. Payan. 1994. Primary culture of gill epithelial cells from the Sea Bass Dicentrarchus labrax In Vitro Cell & Dev. Biol. Anim., Rochville, 30(1):41-49.
  • Eiras-Stofella, D.R. & P. Charvet-Almeida. 1997. Gills of the freshwater fish Hypostomus commersonii Val., 1840 (Loricariidae) analyzed through electron mycroscopy techniques. Arq. Biol. Tecnol., Curitiba, 40 (4):785-792.
  • Fishelson, L. 1980. Scanning and transmission electron microscopy of the squamous gill filament epithelium from fresh - and seawater adapted Tilapia Environ. Biol. Fishes., Dordrecht, 5:161-165.
  • Franklin, C.E. 1990. Surface ultrastructural changes in the gills of sockeie salmon (Teleostei: Oncorhynchus nerka) during seawater transfer. Comparison of sucessful and unsuccessful seawater adaptations. Jour. Morphol., New york, 206:13-23.
  • Godoy, MP. 1975. Peixes do Brasil - Subordem Charachoidei. Bacia do Rio Mogi Guassú. Piracicaba, Franciscana, 4-8:629-847.
  • Hossler, F.E.; J.R. Ruby; T.D. McIlwain. 1979. The gill arch of the mullet Mugil cephalus I. Surface ultrastructure. Jour. Exp. Zool., New York, 208:379-398.
  • Hossler, F.E.; G. Musil; J. Karnaky; F.H. Epstein. 1985. Surface ultrasctruture of the gill arch of the Killifish Fundulus heteroclitus from seawater and freshwater, with special reference to the morphology of apical crypts of chloride cells. Jour. Morphol., New York, 185:377-386.
  • Hossler, F.E.; J.H. Harpole; J.A. King. 1986. The gill arch striped bass Morone saxatilis I. Surface ultrastructure. Jour. Submicrosc. Cytol., Bologna, 18 (3):519-528.
  • Hughes, G.M. 1979. Scanning electron microscopy of the respiratory surface of trout gills. Jour. Zool., London, 187:443-453.
  • ______. 1984. General anatomy of the gills, p.1-72. In: W.S. Hoar & D.J. Randall (Eds). Fish Physiology 10A. New York, Academic Press, XXII+456.
  • Kueltz, D.; J. Juerss; L. Jonas. 1995. Cellular and epithelial adjustments to altered salinity in the gill and opercular epithelium of a cichlid fish (Oreochromis mossambicus). Cell. Tissue Res., Heidelberg, 279 (1):65-73.
  • Lumsden, J.S.; H.W. Ferguson; V.E. Ostland; P.J. Byrne. 1994. The mucous coat on gill lamellae of rainbow trout (Oncorhynchus mykiss). Cell. Tissue. Res., Heidelberg, 275:187-193.
  • Moraes, M.F.P.G.; I.F. Barbola; E.A.C. Guedes. 1997. Alimentação e relações morfológicas com o aparelho digestivo do "curimbatá", Prochilodus lineatus (Valenciennes) (Osteichthyes, Prochilodontidae), de uma lagoa do Sul do Brasil. Revta bras. Zool. 14 (1):169-180.
  • Morgan, M. 1974. Development of secondary lamellae of the gills of the trout Salmo gairdneri (Richardson). Cell Tissue Res., Heidelberg, 151:509-523.
  • Newstead, D. 1967. Fine structure of respiratory lamellae of teleostean gills. Z. Zellforsch. Mikrosk. Anat., Berlin, 79:398-428.
  • Ojha, J. & A.K. Mishra. 1993. Interespecific variations in the functional organization, dimensions and scaling of gills in relation to body weigth of the gangetic mullets, Rhinomugil corsula (Ham.) and Sicamugil cascasia (Ham.) (Mugilidae, Mugiliformes). Proc. Indian Nat. Sci. Acad. Part B: Biol. Sci., Calcuttá, 59 (6):567-579.
  • Ojha, J.; A.K. Mishra; J.S.D. Munshi. 1987. Interespecific variations in the surface ultrastructure of the gills of freshwater mullets. Jpn. Jour. Ichthyol., Tokyo, 33 (4):338-393.
  • Sperry, D.J. & R.J. Wassersug. 1976. A proposed function for microridges on epithelial cell. Anat. Rec., New York, 185:253-258.
  • Whitear, M. 1990. Causative aspect of microridges on the surface of fish epithelia. Jour. Submicros. Cytol. Pathol., Bologna, 22 (2):211-220.

Publication Dates

  • Publication in this collection
    10 July 2009
  • Date of issue
    1998

History

  • Accepted
    17 Apr 1998
  • Received
    28 Feb 1997
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