Dynamics and cytochemistry of oogenesis in Astyanax fasciatus (Cuvier) (Teleostei, Characiformes, Characidae) from Rio Sapucaí, Minas Gerais State, Brazil

Garcia, José Antônio Dias; Chini, Helena A.S.; Maistro, Edson Luis; Quagio-Grassiotto, Irani

doi:10.1590/S0101-81752001000400003

Abstract

Oogenesis involves a set of transformations which are undergone by female germ cells These cells change into oogonias and then into mature oocytes. Sexually mature female fish were collected monthly, during one year, from the Sapucaí River, a tributary of the Rio Grande, which is part of the Furnas Reservoir in the state of Minas Gerais. During the several stages of maturation, we observed small round oogonias with a large nucleus, a single nucleolus, and weakly stained cytoplasm with eosinophilic granules. The primary oocytes showed a large basophilic nucleus, with a developed peripheral nucleolus and a reduced cytoplasm. The previtellogenic oocytes presented voluminous cytoplasm and nucleus with several small peripheral nucleoli. The oocytes underwent vitellogenesis with the development of the zona radiata and the follicle cells. Their cytochemical reactions indicated that the two layers of the zona radiata of A. fasciatus contained proteins and polysaccharides. The initially squamous follicle cells, became cuboidal. In mature oocytes, the nucleus moved toward the periphery, next to the micropyle, and the yolk granules formed by proteins, fulfilled the cytoplasm. The clear unstained vesicles are likely to be the cortical alveoli in the perivitelline region.

Teleostei; Characidae; Astyanax fasciatus; oogenesis; cytochemical

Dynamics and cytochemistry of oogenesis in Astyanax fasciatus (Cuvier) (Teleostei, Characiformes, Characidae) from Rio Sapucaí, Minas Gerais State, Brazil

José Antônio Dias Garcia^I; Helena A.S. Chini^II; Edson Luis Maistro^III; Irani Quagio-Grassiotto^IV

^IFaculdade de Medicina Veterinária, Departamento de Medicina Veterinária Preventiva, UNIFENAS. Caixa Postal 23, 37130-000 Alfenas, Minas Gerais, Brasil

^IIInstituto de Odontologia e Ciências da Saúde, UNIFENAS. Caixa Postal 23, 37130-000 Alfenas, Minas Gerais, Brasil

^IIIInstituto de Farmácia e Nutrição, UNIFENAS. Caixa Postal 23, 37130-000 Alfenas, Minas Gerais, Brasil. E-mail: maistro.edson@unifenas.br

^IVDepartamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista. Campus Rubião Júnior, 18300-000 Botucatu, São Paulo, Brasil

ABSTRACT

Oogenesis involves a set of transformations which are undergone by female germ cells These cells change into oogonias and then into mature oocytes. Sexually mature female fish were collected monthly, during one year, from the Sapucaí River, a tributary of the Rio Grande, which is part of the Furnas Reservoir in the state of Minas Gerais. During the several stages of maturation, we observed small round oogonias with a large nucleus, a single nucleolus, and weakly stained cytoplasm with eosinophilic granules. The primary oocytes showed a large basophilic nucleus, with a developed peripheral nucleolus and a reduced cytoplasm. The previtellogenic oocytes presented voluminous cytoplasm and nucleus with several small peripheral nucleoli. The oocytes underwent vitellogenesis with the development of the zona radiata and the follicle cells. Their cytochemical reactions indicated that the two layers of the zona radiata of A. fasciatus contained proteins and polysaccharides. The initially squamous follicle cells, became cuboidal. In mature oocytes, the nucleus moved toward the periphery, next to the micropyle, and the yolk granules formed by proteins, fulfilled the cytoplasm. The clear unstained vesicles are likely to be the cortical alveoli in the perivitelline region.

Key words: Teleostei, Characidae, Astyanax fasciatus, oogenesis, cytochemical

Full text available only in PDF format.

ACKNOWLEDGEMENTS. The authors are grateful to Mr. Antônio Vicente Salvador and Mr. Ricardo André dos Santos for their technical help, and Dr. Francisco Langeani Filho for identifying the specimens. Funds supporting this study were provided by FAPEMIG, Hidroelétrica de Furnas and Universidade de Alfenas.

Recebido em 31.VIII.2000; aceito em 15.X.2001.

Anderson, E. 1968. Cortical alveoli formation and vitellogenesis during oocyte differentiation in the pipefish, Syngnathus fuscus and the killifish. Fundulus heteroclitus. Jour. Morphol. 125:23-60.
Azevedo, CO.; M.C. Barbieri & G. Barbieri. 1988a. Ciclo reprodutivo de Parodon tortuosus (Eigenmann & Norris, 1900) do Rio Passa Cinco, Ipeúna, SP. I. Estádio de maturação dos testículos. Época de reprodução. Rev. Brasil. Biol. 48:565-569.
_____. 1988b. Ciclo reprodutivo de Parodon tortuosus (Eigenmann and Norris, 1900) do Rio Passa Cinco, Ipeúna. SP. I. Estádio de maturação dos testículos. Época de reprodução. Rev. Brasil. Biol. 48:571-575.
Bazzoli, N. & E. Rizzo. 1990. A comparative cytological and cytochemical study of the oogenesis in tem Brazilian teleost fish species. Eur. Arch. Biol. 101 (4): 399-410.
Bazzoli. N. & HP. Godinho. 1994. Cortical alveoli in oocytes of freshwater neotropical teleost fish. Bol. Zool.61(4):301-308.
______. 1995. Comparative morphogy of the yolk nucleus (Balbiani body) in freshwater neotropical teleost fish. Rev. Brasil Biol. 55:207-214.
______. 1997. Ovócitos vitogênicos do surubim Pseudoplatystoma coruscans e do pacamã Lophiosilurus alexandri. Coleção Meio Ambiente. Belo Horizonte, 19:81-90.
Bazzoli, N.; E. Rizzo; J.E. Santos & Y. Sato. 1996. Dinâmica da ovogênese em peixes forrageiros da represa de Três Marias, Minas Gerais: estudo histológico e histoquímico. BIOS. Belo Horizonte, 4:(4):5-10.
Bristski, H.A. 1972. Peixes de água doce do Estado de São Paulo: Sistemática, p. 79-108. S. M. Branco (Ed.) In. Poluição e Piscicultura. São Paulo, Faculdade de Saúde Pública, USP, Instituto de Pesca CPRN/SA, 216p.
Chaves, P.T.C. & A.E.A.M. Vazzoler. 1984. Aspectos biológicos de peixes amazônicos. II. Anatomia microscópica de ovários, escala de maturidade e tipo de desova das espécies do género Semaprochilodus. Rev. Brasil. Biol. 44:347-359.
Coello, S.A. 1989. New staining schedule for formalin-fixed, glicol methacrylate- embedded fish ovaries. Jour. Fish Biol. 34:329-330.
de Vlaming, V. 1983. Oocyte development patterns and hormonal involvements among teleost, p. 176-199. In: J.C Rankin; T.J. Pitcher & R.T. Duggan (Eds). Control processes in fish physiology. Croom Helm, London & Canberra, 287p.
Karnovsky, J.M. 1964. A formaldehyde-glutaraldehyde fixative of high osmolarity for use in Eletron Microscopy. Jour. Cell Biol. 27:137.
Nagahama, Y. 1983. The functional morphology of teleost gonads, p. 223-276. In: W.S. Hoar; D.J. Randall & EM. Donaldson (Eds). Fish Physiology. 9. Reproduction, part A. New York, Academic Press, 338p.
Neves, C.A.; D.R. Andrade; S.L.P Matta; M.V. Vidal & A.A. Santos. 1995. Cytochemical analysis of polysaccharides from the cortical alveoli of the oocytes of the lambari-bocarra (Oligosarcurs argenteus Günther. 1864) (Pisces, Characidae). Rev. Brasil. Biol. 55:693-696.
Nomura, H. 1984. Dicionário dos peixes do Brasil. Brasília, Editerra, 482p.
Rizzo. E. & N. Bazzoli. 1993. Oogenesis, oocyte surface and micropylar apparatus of Prochilodus affinus Reinhardt, 1874 (Pisces, Characiformes). Eur. Arch. Biol. 104:1-6.
______. 1995. Follicular atresia in Prochilodus affinis Reinhardt. 1874 (Pisces. Characiformes). Rev. Brasil. Biol. 55:(4):697-703.
Selman, K. & R.A. Wallace. 1989. Cellular aspects of oocyte growth in teleosts. Zool. Scien.. CIDADE?, 6:211-231.
Sumpter, J.P.; A.P. Scott; S.M. Baynes & P.R. Witthames. 1994. Early stages of the reproductive cyle in virgin female rainbow trout (Salmo gairdneri Richardson). Aquaculture 43:235-242.
Tyler, C.R. & J.P. Sumpter. 1996. Oocyte growth and development in teleosts. Rev. Fish Biol. Fresheries 6:287-318.
Vazzoler, A.E.A.M. 1996. Biologia da reprodução de peixes telcósteos. Teoria e Prática. Maringá, EDUEM, 169p.
Wallace, R.A. & K. Selman. 1981. Cellular and dynamic aspect of oocyte growth in teleosts. Scien Zool. 21:325-343.
West. G. 1990. Methods of assessing ovarian development in fish: a review. Aust. Jour. Mar. Freshw. Res. 41(2):199-222.
Yamamoto, K. & H Yoshioka. 1964. Rhythm of development in the oocyte of medaka, Oryzias latipes. Bull. Fac. Fish. Hokkaido Univ. 15(1):50-19.

Publication Dates

Publication in this collection
03 June 2009
Date of issue
Dec 2001

History

Received
31 Aug 2000
Accepted
15 Oct 2001

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Anderson, E. 1968. Cortical alveoli formation and vitellogenesis during oocyte differentiation in the pipefish, Syngnathus fuscus and the killifish. Fundulus heteroclitus. Jour. Morphol. 125:23-60.

[2] Azevedo, CO.; M.C. Barbieri & G. Barbieri. 1988a. Ciclo reprodutivo de Parodon tortuosus (Eigenmann & Norris, 1900) do Rio Passa Cinco, Ipeúna, SP. I. Estádio de maturação dos testículos. Época de reprodução. Rev. Brasil. Biol. 48:565-569.

[3] _____. 1988b. Ciclo reprodutivo de Parodon tortuosus (Eigenmann and Norris, 1900) do Rio Passa Cinco, Ipeúna. SP. I. Estádio de maturação dos testículos. Época de reprodução. Rev. Brasil. Biol. 48:571-575.

[4] Bazzoli, N. & E. Rizzo. 1990. A comparative cytological and cytochemical study of the oogenesis in tem Brazilian teleost fish species. Eur. Arch. Biol. 101 (4): 399-410.

[5] Bazzoli. N. & HP. Godinho. 1994. Cortical alveoli in oocytes of freshwater neotropical teleost fish. Bol. Zool.61(4):301-308.

[6] ______. 1995. Comparative morphogy of the yolk nucleus (Balbiani body) in freshwater neotropical teleost fish. Rev. Brasil Biol. 55:207-214.

[7] ______. 1997. Ovócitos vitogênicos do surubim Pseudoplatystoma coruscans e do pacamã Lophiosilurus alexandri. Coleção Meio Ambiente. Belo Horizonte, 19:81-90.

[8] Bazzoli, N.; E. Rizzo; J.E. Santos & Y. Sato. 1996. Dinâmica da ovogênese em peixes forrageiros da represa de Três Marias, Minas Gerais: estudo histológico e histoquímico. BIOS. Belo Horizonte, 4:(4):5-10.

[9] Bristski, H.A. 1972. Peixes de água doce do Estado de São Paulo: Sistemática, p. 79-108. S. M. Branco (Ed.) In. Poluição e Piscicultura. São Paulo, Faculdade de Saúde Pública, USP, Instituto de Pesca CPRN/SA, 216p.

[10] Chaves, P.T.C. & A.E.A.M. Vazzoler. 1984. Aspectos biológicos de peixes amazônicos. II. Anatomia microscópica de ovários, escala de maturidade e tipo de desova das espécies do género Semaprochilodus. Rev. Brasil. Biol. 44:347-359.

[11] Coello, S.A. 1989. New staining schedule for formalin-fixed, glicol methacrylate- embedded fish ovaries. Jour. Fish Biol. 34:329-330.

[12] de Vlaming, V. 1983. Oocyte development patterns and hormonal involvements among teleost, p. 176-199. In: J.C Rankin; T.J. Pitcher & R.T. Duggan (Eds). Control processes in fish physiology. Croom Helm, London & Canberra, 287p.

[13] Karnovsky, J.M. 1964. A formaldehyde-glutaraldehyde fixative of high osmolarity for use in Eletron Microscopy. Jour. Cell Biol. 27:137.

[14] Nagahama, Y. 1983. The functional morphology of teleost gonads, p. 223-276. In: W.S. Hoar; D.J. Randall & EM. Donaldson (Eds). Fish Physiology. 9. Reproduction, part A. New York, Academic Press, 338p.

[15] Neves, C.A.; D.R. Andrade; S.L.P Matta; M.V. Vidal & A.A. Santos. 1995. Cytochemical analysis of polysaccharides from the cortical alveoli of the oocytes of the lambari-bocarra (Oligosarcurs argenteus Günther. 1864) (Pisces, Characidae). Rev. Brasil. Biol. 55:693-696.

[16] Nomura, H. 1984. Dicionário dos peixes do Brasil. Brasília, Editerra, 482p.

[17] Rizzo. E. & N. Bazzoli. 1993. Oogenesis, oocyte surface and micropylar apparatus of Prochilodus affinus Reinhardt, 1874 (Pisces, Characiformes). Eur. Arch. Biol. 104:1-6.

[18] ______. 1995. Follicular atresia in Prochilodus affinis Reinhardt. 1874 (Pisces. Characiformes). Rev. Brasil. Biol. 55:(4):697-703.

[19] Selman, K. & R.A. Wallace. 1989. Cellular aspects of oocyte growth in teleosts. Zool. Scien.. CIDADE?, 6:211-231.

[20] Sumpter, J.P.; A.P. Scott; S.M. Baynes & P.R. Witthames. 1994. Early stages of the reproductive cyle in virgin female rainbow trout (Salmo gairdneri Richardson). Aquaculture 43:235-242.

[21] Tyler, C.R. & J.P. Sumpter. 1996. Oocyte growth and development in teleosts. Rev. Fish Biol. Fresheries 6:287-318.

[22] Vazzoler, A.E.A.M. 1996. Biologia da reprodução de peixes telcósteos. Teoria e Prática. Maringá, EDUEM, 169p.

[23] Wallace, R.A. & K. Selman. 1981. Cellular and dynamic aspect of oocyte growth in teleosts. Scien Zool. 21:325-343.

[24] West. G. 1990. Methods of assessing ovarian development in fish: a review. Aust. Jour. Mar. Freshw. Res. 41(2):199-222.

[25] Yamamoto, K. & H Yoshioka. 1964. Rhythm of development in the oocyte of medaka, Oryzias latipes. Bull. Fac. Fish. Hokkaido Univ. 15(1):50-19.