Estudo histológico e histoquímico da glândula de Duvernoy de Clelia plumbea (Wied) (Serpentes, Colubridae, Xenodontinae)

Histological and histochemical study of Duvernoy's gland from Clelia plumbea (Wied) (Serpentes, Colubridae, Xenodontinae)

Resumo

There are report two human envenenomations for species from Clelia Fitzinger, 1826 (opisthoglyph snake). The patients exhibited symptoms similar from bothropic accident. Then this work have with objective, the hystological and histochemical study of Duvernoy's gland from Clelia plumbea. For the hystological observation of the Duvernoy's gland were using, with paraffin's cutting edge, two methods: Hematoxylin + Eosin and Toluidine's Blue. With historesine's cutting edge were using three methods: Metilen's Blue, Toluidine's Blue + Floxine and Toluidine's Blue. For the histochemical detection were using six techniques, with paraffin's cutting edge: PAS, PAS + Alcian Blue pH 2,5, Alcian Blue pH 2,5, PAS + Salivar Amilase, Method of Lars Grimelius and Method of Lillie. It was evidenced the predominance of serous cells on Duvernoy's gland. In relation to the histochemical constituion of the duvernoy's gland, was verified the presence of glucoconjugates neuters, enzymes and cells with reduetive activity. By fact from this species to kill his preys by constriction, the predominance of serous cells in the duvernoy's gland, don' t must be direct related with the subjugation of his preys, but with the digestive processes. Histological and histochemical analysis showed cells of Duvernoy's gland are constituted for two populations: serous cells (prodution of enzymes) and mucous cells (prodution of glucoconjugates).

Serpentes; Colubridae; Clelia plumbea; Duvernoy's gland; histological; histochemical


Serpentes; Colubridae; Clelia plumbea; Duvernoy's gland; histological; histochemical

Estudo histológico e histoquímico da glândula de Duvernoy de Clelia plumbea (Wied) (Serpentes, Colubridae, Xenodontinae)

Histological and histochemical study of Duvernoy's gland from Clelia plumbea (Wied) (Serpentes, Colubridae, Xenodontinae)

Márcia Ferret RennerI; Simone Maria Teixeira de Sabóia-MoraisII

ICurso de Pós-graduação em Biociências, Departamento de Biologia, Faculdade de Biociências e Museu de Ciências e Tecnologia, Pontifícia Universidade Católica do Rio Grande do Sul. Caixa Postal 1429, 90619-900 Porto Alegre, Rio Grande do Sul, Brasil

IILaboratório de Comportamento Celular. Departamento de Morfologia, Campus II, ICB IV, Universidade Federal de Goiás. Caixa Postal 131, 740001-970 Goiânia, Goiás, Brasil

ABSTRACT

There are report two human envenenomations for species from Clelia Fitzinger, 1826 (opisthoglyph snake). The patients exhibited symptoms similar from bothropic accident. Then this work have with objective, the hystological and histochemical study of Duvernoy's gland from Clelia plumbea. For the hystological observation of the Duvernoy's gland were using, with paraffin's cutting edge, two methods: Hematoxylin + Eosin and Toluidine's Blue. With historesine's cutting edge were using three methods: Metilen's Blue, Toluidine's Blue + Floxine and Toluidine's Blue. For the histochemical detection were using six techniques, with paraffin's cutting edge: PAS, PAS + Alcian Blue pH 2,5, Alcian Blue pH 2,5, PAS + Salivar Amilase, Method of Lars Grimelius and Method of Lillie. It was evidenced the predominance of serous cells on Duvernoy's gland. In relation to the histochemical constituion of the duvernoy's gland, was verified the presence of glucoconjugates neuters, enzymes and cells with reduetive activity. By fact from this species to kill his preys by constriction, the predominance of serous cells in the duvernoy's gland, don' t must be direct related with the subjugation of his preys, but with the digestive processes. Histological and histochemical analysis showed cells of Duvernoy's gland are constituted for two populations: serous cells (prodution of enzymes) and mucous cells (prodution of glucoconjugates).

Key words: Serpentes, Colubridae, Clelia plumbea, Duvernoy's gland, histological, histochemical

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AGRADECIMENTOS. Somos gratos ao Prof. Dr. Thales De Lema e a Paulo Sérgio Bernarde pela leitura e sugestões do manuscrito. A Marta R. Magalhães pelo auxílio no sacrifício das serpentes. Ao Prof. Luciano Vannucci da UFG (Laboratório de Microscopia) do Departamento de Biologia Geral, pelo empréstimo do fotomicroscópio.

Recebido em 07.IV.1999; aceito em 12.VII.2000.

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Datas de Publicação

  • Publicação nesta coleção
    08 Maio 2009
  • Data do Fascículo
    Set 2000

Histórico

  • Aceito
    12 Jul 2000
  • Recebido
    07 Abr 1999
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