ISSR markers were used to discriminate intraspecific peanut accessions, and to utilize the most divergents aiming to generate variability by crossing. The accessions were grown in a greenhouse and at 20 days after sowing, leaves were collected for DNA extraction and subsequent ISSR-PCR tests. Ten primers were used to generate mono and polymorphic bands. Among them, the most contributive to discriminating accessions were UBC-818 and UBC-842, which respectively produced 14 and 12 bands, and a polymorphism rate of 64 and 83%. The primers UBC-847 and UBC-858 generated few bands, with over 60% monomorphic and low contribution to studies of access discrimination using ISSR markers. Three distinct clusters were formed in the dendrogram, among which two genotypes with greater divergence, represented by cultivar BR 1 (short cycle and upright) and line LViPE-06 (late cycle and runner), were selected for hybridization to generate genetic variability. The resulting F2 progenies showed broad genetic variability, with large contribution to selection procedures for the to assist selection procedures in peanut. Based on the results obtained, it was possible to confirm the contribution of molecular tools to assist selection procedures in peanut.
Peanut-breeding; Hybridization
