BACKGROUND: To compare the hepatocyte injury due to the employment of previous ischemic conditioning and two other commonly used maneuvers for clamping the portal triad : continuous clamping and intermittent clamping. METHODS: 40 Wistar rats were divided in 4 groups .On the SHAM group, no clamping was performed. On the other 3 groups , a 40 minutes ischemia was induced by clamping the portal triad. On the Group I we performed a continuous ischemia . On the Group II , before the continuous clamping , a 5 minutes ischemia and a 10 minutes reperfusion was performed (i.e. previous ischemic conditioning).On Group III an intermittent clamping was performed in a 10 minutes ischemia and 5 minutes reperfusion cycle. In order to assess the hepatocyte injury, the Oxalacetic transaminase (SGOT), the pyruvic transaminase (SGPT) , as well the lactic desidrogenase (LDH) were titled before and after the procedures. RESULTS: There was no difference between the values of the starting samples on all the groups , revealing the uniformity of them. The four groups presented significant differences in the starting and ending samples. The odds of SHAM group were lesser significant then in the Group II , that was similar to the group III and in all the groups the rising was significant lower then the Group I (Δ SHAM < Δ Group II = Δ Group III < Δ Group I). CONCLUSION: On Wistar rats , the continuous clamping after the previous ischemic conditioning , induced a minor hepatocyte injury in comparison to the continuous clamping alone and is similar to the intermittent clamping in normal livers, submitted to 40 minutes of hepatic clamping in a whole 60 minutes procedure.
Liver; Ischemia; Reperfusion; Rats; Wistar
