This study aimed to evaluate the effect of different volumes of stationary MS liquid medium on the in vitro multiplication rate and development of banana 'Maçã' explants. During the multiplication phase, were used micro-rhizome of the cultivar 'Maçã' (AAB), derived from pre-established plants on solid culture medium, which were standardized for size. The experimental design was a completely randomized with seven treatments and ten replicates, and the plot consisted of four explants. The treatments were different volumes of the liquid stationary medium (5, 10, 15, 20, 25, 30 mL) and 30 mL of semi-solid medium (standard). Microplants produced at the end of the third subculture were transferred to a growth and rooting medium. The experiment consisted of the same treatments and design, and each plot comprises four explants. The different liquid volume and the semi-solid did not alter the explants multiplication rate. It is possible to produce microplants of banana 'Maçã' in vitro in liquid stationary medium, being the optimal volume of the 25 mL per flask.
multiplication; development; culture medium; Musa spp
