Antioxidant protection of statins in acute kidney injury induced by sepsis

Male adult Wistar rats were divided into the following groups: SHAM (control); SHAM+Statin (0.5 mg/kg simvastatin, orally); Sepsis (cecal puncture ligation – CPL); Sepsis+Statin. Physiological parameters, peritoneal fluid culture, renal function, oxidative metabolites, severity of acute kidney injury and animal survival were evaluated.

Results

The treatment with simvastatin in induced sepsis showed elevation of creatinine clearance with attenuation of generation of oxidative metabolites, lower severity of acute kidney injury and reduced mortality.

Conclusion

This investigation confirmed the renoprotection with antioxidant principle of the simvastatin in acute kidney injury induced by sepsis in an experimental model.

Antioxidants; Acute kidney injury; Sepsis

Grupos	N	Temperature (ºC)	Glycemia (mg/dl)
SHAM	8	36.7±0.6	106.9±8.4
SHAM + Statin	8	36.4±0.3	107.9±8.3
Sepse	8	34.8±0.6^a a p < 0,001 vs SHAM ^b b p < 0,001 vs SHAM + Statin	299.0±7.2^a a p < 0,001 vs SHAM ^b b p < 0,001 vs SHAM + Statin
Sepse + Statin	8	35.1±0.4^a a p < 0,001 vs SHAM ^b b p < 0,001 vs SHAM + Statin	300.3±9.3^a a p < 0,001 vs SHAM ^b b p < 0,001 vs SHAM + Statin

Grupos	N	Urinary Output (ml/ min)	Urine Cr (mg/dl)	Serum Cr (mg/dl)	Crcl/ 100g (ml/ min)
SHAM	8	0.015 ± 0.003	57,66 ± 17.5	0.32 ± 0.08	0.85 ± 0.08
SHAM + Statin	8	0.014 ± 0.002	58,06 ± 12.5	0.35 ± 0.08	0.83 ±0.09
Sepse	8	0.007 ± 0.003^a a p < 0.05 vs SHAM ^b b p < 0.05 vs SHAM + Statin	99,52 ± 37.3	0.93 ± 0.11^a a p < 0.05 vs SHAM ^b b p < 0.05 vs SHAM + Statin	0.22 ± 0.07^a a p < 0.05 vs SHAM ^b b p < 0.05 vs SHAM + Statin
Sepse + Statin	8	0.009 ± 0.002^a a p < 0.05 vs SHAM ^b b p < 0.05 vs SHAM + Statin	49,82 ± 16.1	0.41 ± 0.10^a a p < 0.05 vs SHAM ^c c p < 0.05 vs Sepse	0.46 ± 0.08^a a p < 0.05 vs SHAM ^b b p < 0.05 vs SHAM + Statin ^c c p < 0.05 vs Sepse

Groups	N	Urinary peroxides (nmol/g of UCr)	Urinary TBARS (nmol/g of UCr)
SHAM	8	5.3 ± 1.3	219.5 ± 22.7
SHAM + Statin	8	6.2 ± 2.7	200.6 ± 26.3
Sepse	8	20.3 ± 5.8^a a p < 0,001 vs SHAM ^b b p < 0,001 vs SHAM + Statin	945.6 ± 26.9^a a p < 0,001 vs SHAM ^b b p < 0,001 vs SHAM + Statin
Sepse + Statin	8	11.6 ± 3.8^a a p < 0,001 vs SHAM ^b b p < 0,001 vs SHAM + Statin ^c c p < 0,001 vs Sepse	616.8 ± 25.8^a a p < 0,001 vs SHAM ^b b p < 0,001 vs SHAM + Statin ^c c p < 0,001 vs Sepse

Universidade de São Paulo, Escola de Enfermagem Av. Dr. Enéas de Carvalho Aguiar, 419 , 05403-000 São Paulo - SP/ Brasil, Tel./Fax: (55 11) 3061-7553, - São Paulo - SP - Brazil
E-mail: reeusp@usp.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] Correspondence: Cassiane Dezoti da Fonseca Av. Dr. Enéas de Carvalho Aguiar, 419  CEP 05403-000 - São Paulo, SP, Brazil. cassianedezoti@usp.br