BRIEF REPORT
Entamoeba histolytica zymodemes in children of Osasco, São Paulo
Ivanize da Silva AcaI; Expedito França Jr.II; Tomoyoshi NozakiIII; Giselia Barros FreitasIV; Seiki TatenoIII
IDepartamento de Medicina Tropical and Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco. Recife, PE, Brasil
IIIn memoriam. Research student of the Laboratório de Imunopatologia Keizo Asami. Recife, PE, Brasil
IIIDepartment of Parasitology and Tropical Medicine, School of Medicine, Keio University, Shinjuku-Ku, Tokyo 160 Japan
IVHospital Municipal de Pirituba, São Paulo, Brasil
Address for correspondence Address for correspondence: Prof. Ivanize da Silva Aca Departamento de Medicina Tropical, Centro de Ciências da Saúde. Universidade Federal de Pernambuco Av. Prof. Moraes Rego, s/nº Cidade Universitária. 50730 Recife, PE, Brasil
Entamoeba histolytica is an intestinal human parasite protozoa that can be found either as a non pathogenic commensal or at tissues, causing hemorrhagic colitis or extra-intestinal abscess.
SARGEAUNT et al. (1978,1984,1987) established that E. histolytica can be differentiated in pathogenic and non pathogenic strains by electrophoretic analysis of its isoenzymes. Twenty two strains have been identified from the zymodemes and only nine were recognized as associated to tissue invasion (SARGEAUNT & WILLIAMS, 1978; SARGEAUNT, 1987, 1988; BLANC et al., 1989).
In Brazil, NOZAKI et al. (1990) studied the zymodemes of E. histolytica, obtained from low income people living in the Amazonas region (Belém and Manaus) and Northeast (Recife and São Luiz). In the Amazonas region, pathogenic and non pathogenic strains were isolated whereas in the Northeast only non pathogenic strains were identified.
In this work, fecal samples were collected from 155 asymptomatic children studying at creche of Osasco (Irmã Benedita Constancia, Santo Antonio and Parque Infantil), from May to June of 1989. They aged from 1 to 15 years.
The fecal samples were microscopically analyzed for the presence of E. histolytica cysts or trophozoites as well as for other enteroparasites. The following intestinal parasites were found in fifty four (35%) of the 155 examined children: Giardia lamblia (25%), Ascaris lumbricoides (7%), Entamoeba coli (8%), Entamoeba hartmanni (11%), Endolimax nana (4%), Trichuris trichiura (3%), Entamoeba histolytica (2.5%), Hymenolepis nana (1%), Necator americanus (0.6%) and Dientamoeba fragilis (0.6%).
An aliquot of each fecal sample was directly inoculated into Robinson medium (ROBINSON, 1968) and incubated at 37ºC. All positive cultures were electrophoretically analyzed for the zymodemes according to SARGEAUNT (SARGEAUNT & WILLIAMS, 1978; SARGEAUNT, 1988). The enzymes used as markers were glucose-phosphate-isomerase (GPI), Phospho-glucomutase (PGM), Hexoquinase (HK) and L-malato-NADP+ oxidoreductase (oxalacetatedecarboxilase) (ME). The zymodemes were compared with the standard strains SAW 1015 (pathogenic) and SAW 1719 (non pathogenic). Four fecal samples were positive for E. histolytica and all of them were characterized according to the zymodemes as non pathogenic strain, type I (Figures 1-4).
The prevalence of intestinal parasites, including E. histolytica, observed in this work reproduced that reported by CHIEFFI et al. (1988). However, those researches did not characterized the strains of E. histolytica according to their pathogenicity. In Pernambuco, Northeast of Brazil, the prevalence of E. histolytica is higher (14 to 36%) than São Paulo (KOBAYASHI et al., 1991). This can be attributed to the poor sanitation conditions, lack of hygiene and basic sanitation which are more critical in the Northeast of Brazil. Nevertheless, the isolated strains of E. histolytica were also non pathogenic.
Studies are in progress in order to correlate symptoms and pathogenicity of E. histolytica.
ACKNOWLEDGMENTS
This work was supported by the Japan International Cooperation Agency (JICA).
Recebido para publicação em 14/01/1993
Aceito para publicação em 15/07/1993
- 1. BLANC, D. & SARGEAUNT, P.D. - Entamoeba histolytica zymodemes: exhibition of and bands only of glucose phosphate isomerase and phosphoglucomutase may be influenced by starch content in the medium. Exp. Parasit., 72: 87-90, 1991.
- 2. CHIEFFI, P.P.; WALDMAN, E.A.; DIAS, R.M.D.S.; TORRES, D.M.A.G.V.; CHIMARA, R.; MIZUMOTO, L.; SILVA, A.A. & UEHARA, M. - Enteroparasitoses no município de Guarulhos, SP, Brasil. 1. Prevalęncia de infecçăo entre escolares residentes no Bairro de Taboăo em junho de 1984. Rev. Inst. Adolfo Lutz, 48 (1-2): 75-80, 1988.
- 3. GONÇALVES, J.F.; TANABE, M.; MEDEIROS, F.P.M.; GONÇALVES, F.J.; ACA, I.S.; MOTTA, S.R.N.; TATENO, S. & TAKEUCHI, T. - Parasitological and serological studies on amoebiasis and other intestinal parasitic infections in the rural sector around Recife, Northeast Brazil. Rev. Inst. Med. trop. S. Paulo, 32: 428-435, 1990.
- 4. KOBAYASHI, S.; ACA, I.S.; PAULA, F.; MELO, V.; TACHIBANA, II.; KANEDA, Y.; TATENO, S. & TAKEUCHI, T. - Distribution of nonpathogenic strains of E. histolytica in Pernambuco, northeast Brazil. Jap. J. Parasit., 40 (supl.): 73, 1991.
- 5. NOZAKI, T.; ACA, I.S.; OKUZAWA, E.; MAGALHĂES, M.; TATENO, S. & TAKEUCHI, T. - Zymodemes of Entamoeba histolytica isolated in the Amazon and the Northeast of Brazil. Trans. roy. Soc. trop. Med. Hyg., 84: 387-388, 1990.
- 6. OKAZAKI, M.; MIRANDA, P.; NETO, J.; DIEGUES, V.; ALVES, J.; CAUAS, M; TANABE, M.; KOBAYASHI, S.; TATENO, S. & TAKEUCHI, T. - Parasitological and serological studies on amoebiasis and other intestinal parasitic infections in Recife and its suburban area, Northeast Brazil. Rev. Inst. Med. trop. S. Paulo, 30: 313-321, 1988.
- 7. ROBINSON, G.I. - The laboratory diagnosis of human parasitic amoeba. Trans. roy. Soc. trop. Med. Hyg., 62: 285-293, 1968.
- 8. SARGEAUNT, P.D. - The reability of Entamoeba histolytica zymodemes in clinical diagnosis. Parasit. today, 3: 40-43, 1987.
- 9. SARGEAUNT, P.G. - Zymodemes of Entamoeba histolytica In: RAVDIN, J.I., ed. Amoebiasis. New York, Churchill Livingstone, 1988. p. 370-387.
- 10. SARGEAUNT, P.G. & WILLIAMS, J.E. - Electrophoretic isoenzyme patterns of Entamoeba histolytica and Entamoeba coli. Trans. roy. Soc. trop. Med. Hyg., 72: 164-166, 1978.
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Publication Dates
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Publication in this collection
03 July 2006 -
Date of issue
Dec 1993
History
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Accepted
15 July 1993 -
Received
14 Jan 1993