Molecular identification of <i>Dermatobia hominis</i> (Diptera: Oestridae): a neglected agent causing myiasis in Mexico

Myiasis represents a group of neglected tropical diseases caused by the infestation of vertebrate tissues by dipterous larvae. We herein report an imported case of foruncular myasis caused by Dermatobia hominis in Mexico City. The species was confirmed by DNA sequencing and phylogenetic reconstruction analysis.

KEYWORDS:
Myiasis; Bot fly; Colmoyote; Cuterebrinae; Cytochrome oxidase I ( cox 1); Dermatobia hominis

Authorship

Yokomi N. Lozano-Sardaneta Correspondence to: Yokomi N. Lozano-Sardaneta, Hospital General de México, Dr. Balmis 148, Col. Doctores, 06726 CDMX, México Tel: +52 55 5623 2674E-mail:nisei_sardaneta@ciencias.unam.mx

Universidad Nacional Autónoma de México, Facultad de Medicina, División de Investigación, Centro de Medicina Tropical, Ciudad de México, MéxicoUniversidad Nacional Autónoma de MéxicoMéxicoCiudad de México, MéxicoUniversidad Nacional Autónoma de México, Facultad de Medicina, División de Investigación, Centro de Medicina Tropical, Ciudad de México, México

http://orcid.org/0000-0002-2389-6087

Sokani Sánchez-Montes

Universidad Veracruzana, Facultad de Ciencias Biológicas y Agropecuarias, Veracruz, MéxicoUniversidad VeracruzanaMéxicoVeracruz, MéxicoUniversidad Veracruzana, Facultad de Ciencias Biológicas y Agropecuarias, Veracruz, México

http://orcid.org/0000-0001-6316-2187

Edith Fernández-Figueroa

National Institute of Genomic Medicine, Department of Population Genomics, Computational and Integrative Genomics, Ciudad de México, MéxicoNational Institute of Genomic MedicineMéxicoCiudad de México, MéxicoNational Institute of Genomic Medicine, Department of Population Genomics, Computational and Integrative Genomics, Ciudad de México, México

Claudia Rangel-Escareño

Tecnologico de Monterrey, Basic Sciences, Queretaro, MéxicoTecnologico de MonterreyMéxicoQueretaro, MéxicoTecnologico de Monterrey, Basic Sciences, Queretaro, México

Ingeborg Becker

Correspondence to: Yokomi N. Lozano-Sardaneta, Hospital General de México, Dr. Balmis 148, Col. Doctores, 06726 CDMX, México Tel: +52 55 5623 2674E-mail:nisei_sardaneta@ciencias.unam.mx

AUTHORS’ CONTRIBUTIONS

YNLS performed the PCR, analyses of sequences, writing and reviewing; SSM and IB were responsible for the clinical attention to patients; EFF and CRE performed the purification of amplicons, and sequencing assays; SSM, EFF, CRE and IB contributed with critical comments. All the authors participated during the discussion and writing of the manuscript and approved its final version.

CONFLICT OF INTERESTS

The authors declare that they have no competing interests.

SCIMAGO INSTITUTIONS RANKINGS

Tecnologico de Monterrey, Basic Sciences, Queretaro, MéxicoTecnologico de MonterreyMéxicoQueretaro, MéxicoTecnologico de Monterrey, Basic Sciences, Queretaro, México

Figures

Figures (2)

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Figure 1
Map of the Mexican territory, highlighting the jungle region with a red star in the State of Chiapas.

Thumbnail

Figure 2
Molecular identification of Dermatobia irritans: A) cox 1. PCR product of the bot fly line 3 (4 μL), Nuclease-free water as the negative control (line 2). L=Ladder 100 pb; B) Neighbor-joining tree with Cytochrome Oxidase I ( cox1 ) sequences of the fly species causing myasis. Numbers on the branches indicate the bootstrap values and the blue diamond is the sequence obtained in this study.

Thumbnail

Figure 1 Map of the Mexican territory, highlighting the jungle region with a red star in the State of Chiapas.

Figure 2 Molecular identification of Dermatobia irritans: A) cox 1. PCR product of the bot fly line 3 (4 μL), Nuclease-free water as the negative control (line 2). L=Ladder 100 pb; B) Neighbor-joining tree with Cytochrome Oxidase I ( cox1 ) sequences of the fly species causing myasis. Numbers on the branches indicate the bootstrap values and the blue diamond is the sequence obtained in this study.

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Molecular identification of <i>Dermatobia hominis</i> (Diptera: Oestridae): a neglected agent causing myiasis in Mexico

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[1] Correspondence to: Yokomi N. Lozano-Sardaneta, Hospital General de México, Dr. Balmis 148, Col. Doctores, 06726 CDMX, México Tel: +52 55 5623 2674E-mail:nisei_sardaneta@ciencias.unam.mx