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Malaria at Humaita county, Amazonas state, Brazil: XVII — immune response in patients with Plasmodium falciparum according to gametocytes

Malária no município de Humaitá, estado do Amazonas: XVII — resposta imune em doentes com infecção pelo Plasmodium falciparum em relação aos gametócitos

Domingos Alves Meira Paulo Roberto Curi Jussara Marcondes Elinda Satie Matsuoka Marina A. Favrin Albert Boutros El-Khoury Norma Gerusa da Silva Motta About the authors

Abstracts

In August 1983 the Authors studied 36 patients with Plasmodium falciparum malaria and 14 normal individuals born in Humaita region who had never had malaria, had no spleen enlargement and had negative parasitemia as well as passive hemagglutination. Medical histories were obtained and complete physical examination were performed in all of them just as blood tests, parasite density and lymphocyte typing. The lymphocytes were separated and then frozen in liquid nitrogen for later typing by rosette formation. The patients were divided in two groups according to the presence (13 patients) or abscence (23 patients) of gametocytes before treatment. Severe malaria was predominant in the group without gametocytes. The results showed a decrease in the T-cell numbers in Plasmodium falciparum acute malaria patients both with or without gametocytes before the treatment, while B-cell numbers were normal only in the patients with gametocytes. These observations as like as those previously reported by the Authors, permit to associate the presence of gametocytes in peripheral blood and normal number of B-cells in patients with mild Plasmodium falciparum malaria.


Em agosto de 1983, os Autores, estudaram 36 doentes com infecção causada pelo Plasmodium falciparum e 14 indivíduos normais, nascidos na região de Humaitá, que nunca tiveram malária, sem esplenomegalia, com exame parasitológico de sangue e hemaglutinação passiva negativas. Todos eles foram submetidos à observação clínica completa, exame hematológico, exame parasitológico de sangue e tipagem de linfócitos. Os linfócitos foram isolados e congelados em nitrogênio líquido, para posterior tipagem pela formação de rosetas. Os doentes foram classificados em dois grupos de acordo com a presença (13 doentes), ou ausência (23 doentes) de gametócitos antes do tratamento. Houve predomínio de formas graves no grupo de doentes sem gametócitos. Os resultados mostraram diminuição do número de linfócitos T em ambos os grupos de doentes, com ou sem gametócitos antes do tratamento, enquanto que o número de linfócitos B foi normal apenas no grupo de doentes com gametócitos. Essas observações, assim como as que foram relatadas pelos Autores anteriormente, permitem associar a presença de gametócitos circulantes com o número normal de células B em doentes com formas leves de malária causada pelo Plasmodium falciparum.


ORIGINAL ARTICLES

Malaria at Humaita county, Amazonas state, Brazil. XVII immune response in patients with Plasmodium falciparum according to gametocytes

Malária no município de Humaitá, estado do Amazonas. XVII — resposta imune em doentes com infecção pelo Plasmodium falciparum em relação aos gametócitos

Domingos Alves MeiraI; Paulo Roberto CuriII; Jussara MarcondesIII; Elinda Satie MatsuokaI; Marina A. FavrinIV; Albert Boutros El-KhouryV; Norma Gerusa da Silva MottaVI

IHead professor of the Department of Infectious and Parasitologic Diseases, Dermatology and Radiology - School of Medicine — Botucatu — UNESP

IIDoctor of the Department of Surgery School of Medicine — Botucatu — UNESP

IIIInstructor of the Department of Infectious and Parasitologic Diseases, Dermatology and Radiology — School of Medicine — Botucatu — UNESP

IVBiologist of São Paulo State Public Functionary Hospital

VHead of the Department of Blood Bank of São Paulo State Public Functionary Hospital

VIDoctor of the Department of Microbiology and Immunology of Basic Institute of Medical and Agricultural Biology — UNESP

SUMMARY

In August 1983 the Authors studied 36 patients with Plasmodium falciparum malaria and 14 normal individuals born in Humaita region who had never had malaria, had no spleen enlargement and had negative parasitemia as well as passive hemagglutination. Medical histories were obtained and complete physical examination were performed in all of them just as blood tests, parasite density and lymphocyte typing. The lymphocytes were separated and then frozen in liquid nitrogen for later typing by rosette formation. The patients were divided in two groups according to the presence (13 patients) or abscence (23 patients) of gametocytes before treatment. Severe malaria was predominant in the group without gametocytes. The results showed a decrease in the T-cell numbers in Plasmodium falciparum acute malaria patients both with or without gametocytes before the treatment, while B-cell numbers were normal only in the patients with gametocytes. These observations as like as those previously reported by the Authors, permit to associate the presence of gametocytes in peripheral blood and normal number of B-cells in patients with mild Plasmodium falciparum malaria.

RESUMO

Em agosto de 1983, os Autores, estudaram 36 doentes com infecção causada pelo Plasmodium falciparum e 14 indivíduos normais, nascidos na região de Humaitá, que nunca tiveram malária, sem esplenomegalia, com exame parasitológico de sangue e hemaglutinação passiva negativas. Todos eles foram submetidos à observação clínica completa, exame hematológico, exame parasitológico de sangue e tipagem de linfócitos. Os linfócitos foram isolados e congelados em nitrogênio líquido, para posterior tipagem pela formação de rosetas. Os doentes foram classificados em dois grupos de acordo com a presença (13 doentes), ou ausência (23 doentes) de gametócitos antes do tratamento. Houve predomínio de formas graves no grupo de doentes sem gametócitos. Os resultados mostraram diminuição do número de linfócitos T em ambos os grupos de doentes, com ou sem gametócitos antes do tratamento, enquanto que o número de linfócitos B foi normal apenas no grupo de doentes com gametócitos. Essas observações, assim como as que foram relatadas pelos Autores anteriormente, permitem associar a presença de gametócitos circulantes com o número normal de células B em doentes com formas leves de malária causada pelo Plasmodium falciparum.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

ACKNOWLEDGEMENTS

We wish to thank the aid of Fundação Projeto Rondon, Directory of Advanced Campus and Humaitá — SUCAM.

Recebido para publicação em 12/11/1984.

Research accomplished in the School of Medicine - Botucatu - UNESP, with support of National Council of Scientific and Technological Development — CNPq (Proc. 40.3705/82) and presented in "The Fourth Japan-Brazil Symposium on Science and Technology" in Botucatu, São Paulo, Brazil, 1984.

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Publication Dates

  • Publication in this collection
    26 Oct 2012
  • Date of issue
    Oct 1985

History

  • Received
    12 Nov 1984
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