Paracoccidioidomycosis in children in the state of Rio de Janeiro (Brazil): Geographic distribution and the study of a "reservarea"

GONÇALVES, A. J. RIOS; LONDERO, A. T.; TERRA, G. M. F.; ROZENBAUM, R.; ABREU, T. F.; NOGUEIRA, S. A.

doi:10.1590/S0036-46651998000100003

Abstracts

Thirty six cases of acute disseminated paracoccidioidomycosis in 3 to 12 year-old children, natives of the state of Rio de Janeiro, were seen in the period 1981-1996. All patients were residents in the rural region of 15 counties, scattered on the Southwestern part of this state. The rural region of two neighboring counties, where 16 cases (44.4%) occurred, was visited. It exhibited the environmental conditions that are considered favorable to the survival of P. brasiliensis. The most important of these conditions, abundant watercourses and autochthonous forest, are distributed on well defined and limited areas, in which the dwellings are also localized. Probably, a careful epidemiological study of forthcoming cases of the disease in children may facilitate the search for the micro-niche of the fungus.

Paracoccidioidomycosis; Paracoccidioides brasiliensis; Ecology

No período 1981-1996 foram observados 36 casos de paracoccidioidomicose aguda disseminada em crianças, entre 3 e 12 anos de idade, nativas do estado do Rio de Janeiro. Todos os pacientes residiam em região rural, distribuída por 15 municípios localizados na parte sudoeste do estado. Dois municípios vizinhos, onde haviam ocorrido 16 (44,4%) dos casos, foram visitados. Na região rural de ambos, existem as condições climáticas e ambientais favoráveis à sobrevivência do P. brasiliensis. Dentre essas condições, as mais importantes, abundantes cursos de água e vegetação nativa, distribuem-se em áreas limitadas e bem definidas. Nessas áreas localizam-se, também, as casas dos rurículas. Provavelmente, um cuidadoso estudo epidemiológico de novos casos, que venham a ocorrer na região, facilitará a busca do nicho do fungo

Paracoccidioidomycosis in children in the state of Rio de Janeiro (Brazil). Geographic distribution and the study of a "reservarea"

A. J. RIOS GONÇALVES(¹ Recebido para publicação em 04/08/1997 Aceito para publicação em 29/12/1997 ), A. T. LONDERO(² Recebido para publicação em 04/08/1997 Aceito para publicação em 29/12/1997 ), G. M. F. TERRA(¹ Recebido para publicação em 04/08/1997 Aceito para publicação em 29/12/1997 ), R. ROZENBAUM(¹ Recebido para publicação em 04/08/1997 Aceito para publicação em 29/12/1997 ), T. F. ABREU(³ Recebido para publicação em 04/08/1997 Aceito para publicação em 29/12/1997 ) & S. A. NOGUEIRA(³ Recebido para publicação em 04/08/1997 Aceito para publicação em 29/12/1997 )

SUMMARY

Thirty six cases of acute disseminated paracoccidioidomycosis in 3 to 12 year-old children, natives of the state of Rio de Janeiro, were seen in the period 1981-1996. All patients were residents in the rural region of 15 counties, scattered on the Southwestern part of this state.

The rural region of two neighboring counties, where 16 cases (44.4%) occurred, was visited. It exhibited the environmental conditions that are considered favorable to the survival of P. brasiliensis. The most important of these conditions, abundant watercourses and autochthonous forest, are distributed on well defined and limited areas, in which the dwellings are also localized.

Probably, a careful epidemiological study of forthcoming cases of the disease in children may facilitate the search for the micro-niche of the fungus.

Keywords: Paracoccidioidomycosis; Paracoccidioides brasiliensis; Geographic distribution; Ecology.

INTRODUCTION

Paracoccidioidin skin test surveys, covering representative samples of rural population in the State of Rio de Janeiro, revealed that positive reactors have been found at less than four years of age⁸, and this may reach to 34.3% of the students 6 to 11 year of age¹². These results suggest that human being may contact Paracoccidioides brasiliensis at an early age. Reported cases of overt-disease that affected 3 year-old children confirm this assumptiom^{2, 11, 14, 15}.

Children of an early age usually develop their activities within a limited area around their houses. Consequently, they represent a good guide to localize the areas where P. brasiliensis survives in nature the "reservareas"^{4, 6}.

"Reservarea" is a term coined by BORELLI³ to designate the places where the fungus lives and man acquires the infection. "Reservareas" have been delimited on the basis of data exhaustively obtained on the residential and occupational patterns of adult patients^{7, 10}, or patients at any age¹⁶. However, recently, CADAVID & RESTREPO⁴ used intradermal test with paracoccidioidin to delimit "reservareas" in a Colombian province, because a case of paracoccidioidomycosis (PCM) had occurred in a child in each one of the "reservareas".

The opportunity to review the clinical records of 36 cases of overt disease in 3 to 12 year-old native children, admitted to hospitals in Rio de Janeiro, led us to present this paper.

PATIENTS

Thirty six 3 to 12 year-old children with acute disseminated PCM were admitted to 3 hospitals of the city of Rio de Janeiro, during the period 1981-1996. All these patients were natives of and residents in the State of Rio de Janeiro.

Sixteen patients (9 males and 7 females) came from two neighboring counties: Barra Mansa and Volta Redonda. The remaining 20 patients came from others 13 counties. These counties were distributed on the Southwestern part of the state.

All patients lived in rural areas, but the exact localization of their homes was not recorded.

SELECTION OF STUDIED AREA

Two neighboring counties were selected for study Barra Mansa and Volta Redonda because nearly half of the 36 patients (16-44.4%) came from these counties. In addition, a survey with paracoccidioidin skin test had been carried out in Barra Mansa¹². This survey revealed the highest rate of positive reactors (60.6%) in a representative rural population in Brazil.

The two counties are located in the middle of the large Paraiba do Sul River Valley.

This valley lies between two chains of mountains, named Serra da Mantiqueira and Serra do Mar. The river flows northwestward to the Atlantic Ocean. Both counties together cover 784.6 km² and are crossed by the river. With the exception of a narrow plain along the border of the river, their topography is of a hilly upland. Along the numerous streams that flow into the Paraiba do Sul river, the region is dissected into a series of rolling hills. With a mean altitude of 380 m above the sea level, some hills may reach as much as 900 m at the foot hill of the Serra da Mantiqueira. The temperature ranges 8° to 40°C, with mean of 23°C; annual precipitation is from 1100 to 1300 mm³; average humidity hovers around 75%.

In the past, this region was covered by a semideciduous forest. With the introduction of the coffee monoculture, in 1883, a progressive deforestation was started. Only narrow strips, bordering the numerous streams, and few small areas covered by native forest were preserved. A few years later, in 1888, soil depletion led to gradual substitution of coffee plantations for pastures⁶. As a consequence cattle breeding, especially dairy cattle, is the main rural activity today. Crop production has been reduced to small areas of sugar cane for forage, vegetable gardening and fruit growing for local consumption.

In the rural region the houses in the small village and also the isolated dwellings have been built alongside the numerous streams that flow into the Paraiba do Sul river. Many small dams have been constructed for water retention or for the stream-crossing machines.

DISCUSSION

In 1976, PEDROSA¹² carried out a survey with paracoccidioidin skin test on a representative population of Barra Mansa. using FAVA NETOs paracoccidioidin diluted 1:80, he obtained the highest rate of positive reactors among the Brazilian rural population. PEDROSA selected this county because a case of acute disseminated PCM had been diagnosed in a native child. Probably, due to this reason, he also presented the results observed in 6 to 11 year-old native students (34.3%). In addition, impressed by the topography of the county, he tabulated the data obtained by testing children less than 11 years of age that lived in the districts of Barra Mansa. He obtained a rate of infected children varying from 25.2% in those living in the district at the level of the river Paraiba do Sul (altitude 380 m) up to 73.4% of those residents in a district situated on the foot hill of the Serra da Mantiqueira (altitude 900 m). Coincidentally, in the counties of Barra Mansa and neighboring Volta Redonda occurred the highest number of cases of overt disease in children.

As PCM affecting children is the result of the primary infection, latency period must be very short^{5, 9}. In addition, children, especially at an early age, carry out their activities on limited area around their houses, and family migration is uncommon, they are the best guide to point out the true "reservarea". One may presume that the niche where P. brasiliensis lives may be located near the patients home. Some facts agree with this presumption: 1) the first conclusive isolation of the fungus, performed by ALBORNOZ¹, was from the soil of a hut in the thorpe of Taica (Venezuela); 2) CADAVID & RESTREPO⁴ demonstrated that housekeeping activities are statistically associated with the infection by P. brasiliensis in a Colombian "reservarea"; and, 3) VERAS¹⁶ commented that children have limited areas of activities in which they can disturb the niche of the fungus.

RESTREPO¹³ and CADAVID & RESTREPO⁴ have called attention to the fact that, besides climatic conditions, the abundance of watercourses bordered by forest are the most important factors for supporting the P. brasiliensis micro-niche. In the counties of Barra Mansa and Volta Redonda there are numerous streams bordered by a narrow band of native vegetation, where are also located the villages and isolated houses. In this "reservarea" a through epidemiological study of forthcoming cases of overt disease in children can point out to those areas where the fungus can be more easily pursued.

RESUMO

Paracoccidioidomicose em crianças no Estado do Rio de Janeiro (Brasil). Distribuição geográfica e análise de uma "reservarea"

No período 1981-1996 foram observados 36 casos de paracoccidioidomicose aguda disseminada em crianças, entre 3 e 12 anos de idade, nativas do estado do Rio de Janeiro. Todos os pacientes residiam em região rural, distribuída por 15 municípios localizados na parte sudoeste do estado.

Dois municípios vizinhos, onde haviam ocorrido 16 (44,4%) dos casos, foram visitados. Na região rural de ambos, existem as condições climáticas e ambientais favoráveis à sobrevivência do P. brasiliensis. Dentre essas condições, as mais importantes, abundantes cursos de água e vegetação nativa, distribuem-se em áreas limitadas e bem definidas. Nessas áreas localizam-se, também, as casas dos rurículas.

Provavelmente, um cuidadoso estudo epidemiológico de novos casos, que venham a ocorrer na região, facilitará a busca do nicho do fungo.

(1) Hospital dos Servidores do Estado, MS, Rio de Janeiro, RJ, Brasil.

(2) Universidade Federal de Santa Maria, RS, Brasil.

(3) Departamento de Doenças Infecciosas e Parasitárias. Universidade Federal do Rio de Janeiro, RJ, Brasil.

Correspondence to: A. T. Londero M. D., Venancio Aires 2020/404, 97010-004 Santa Maria, RS, Brasil.

1. ALBORNOZ, M. B. Isolation of Paracoccidioides brasiliensis from rural soil in Venezuela. Sabouraudia, 9: 248-253, 1971.
2. BARBOSA, G. L. Paracoccidioidomicose na criança. Rev. Pat. trop., 21: 269-363, 1992.
3. BORELLI, D. Some ecological aspects of paracoccidioidomycosis. In: Paracoccidioidomycosis PROCEEDINGS OF THE FIRST PAN AMERICAN SYMPOSIUM, Medellin, 1971. Pan. Amer. Hlth. Org. Scient. Publ., (254): 59-64, 1972.
4. CADAVID, D. & RESTREPO, A. Factors associated with Paracoccidioides brasiliensis infection among permanent residents of three endemic areas in Colombia, SA. Epidem. infect., 111: 121-133, 1993.
5. CAMPOS, E. P.; BERTOLI, C. I. & BARBOSA, K. S. Linfonodo pulmonar na paracoccidioidomicose aguda infantil. (Relato de um caso). Rev. Soc. bras. Med. trop., 25: 195-200, 1992.
6. CARDOSO, M. F. T. C. Organizaçăo e reorganizaçăo do espaço do Paraíba do Sul. Uma análise geográfica até 1940. Rev. bras. Geogr., 58: 81-135, 1991.
7. FORJAZ, M. H. H. Estudo epidemiológico da paracoccidioidomicose. Rastreamento de áreas endęmicas e de "reservareas" no Brasil através do traçado do perfil migratório-residencial-profissional de pacientes diagnosticados em Săo Paulo. Săo Paulo, 1989. (Tese de Doutorado - Escola Paulista de Medicina).
8. FREITAS, N. A. Paracoccidioidomicose. Inquérito com paracoccidioidina e busca ativa de casos no Município de Campos, RJ. Rio de Janeiro, 1992. (Dissertaçăo de Mestrado - Universidade Federal do Rio de Janeiro).
9. GIRALDO, R.; RESTREPO, A.; GUTIERREZ, F. et al. Pathogenesis of paracoccidioidomycosis: a model based on the study of 46 patients. Mycopathologia (Den Haag), 58: 63-70, 1976.
10. LONDERO, A. T.; RAMOS, C. D. & LOPES, J. O. "Reservarea" de paracoccidioidomicose no Rio Grande do Sul, Brasil. Rev. Inst. Med. trop. S. Paulo, 14: 377-380, 1972.
11. MARTINS, R. M.; MARQUES, J. L. O.; GARCEZ, I. C. S. et al. Paracoccidioidomicose. Relato de caso em criança de tręs anos de idade com a forma disseminada. J. Pediat., 61: 319-322, 1986.
12. PEDROSA, P. N. Paracoccidioidomicose. Inquérito intradérmico com paracoccidioidina em zona rural do Estado do Rio de Janeiro. Rio de Janeiro, 1976. (Dissertaçăo de Mestrado - Universidade Federal do Rio de Janeiro).
13. RESTREPO, A. The ecology of Paracoccidioides brasiliensis A puzzle still unsolved. J. med. vet. Mycol., 23: 323-334, 1985.
14. RIOS-GONÇALVES, A. J.; TERRA, G. M. F.; ROZENBAUM, R. et al. Paracoccidiodomicose em criança de tręs anos de idade. Apresentaçăo de dois casos. Arq. bras. Med., 70: 73-79, 1996.
15. ROSARIO-FILHO, N. A.; QUEIROZ-TELLES Fo, F.; COSTA, O. et al. Paracoccidioidomycosis in children with different skeletal involvement. Rev. Inst. Med. trop. S. Paulo, 27: 337-340, 1985.
16. VERAS, K. N. Paracoccidioidomicose. Estudo epidemiológico e clínico de pacientes internados no Hospital de Doenças Infecto Contagiosas em Teresina, Piauí. Identificaçăo de reservareas no Estado do Pará e Maranhăo. Teresina, 1995. (Dissertaçăo de Mestrado - Instituto Oswaldo Cruz, FIOCRUZ, RJ).

Recebido para publicação em 04/08/1997

Aceito para publicação em 29/12/1997

Publication Dates

Publication in this collection
16 June 1999
Date of issue
Jan 1998

History

Received
04 Aug 1997
Accepted
29 Dec 1997

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ALBORNOZ, M. B. Isolation of Paracoccidioides brasiliensis from rural soil in Venezuela. Sabouraudia, 9: 248-253, 1971.

[2] 2. BARBOSA, G. L. Paracoccidioidomicose na criança. Rev. Pat. trop., 21: 269-363, 1992.

[3] 3. BORELLI, D. Some ecological aspects of paracoccidioidomycosis. In: Paracoccidioidomycosis PROCEEDINGS OF THE FIRST PAN AMERICAN SYMPOSIUM, Medellin, 1971. Pan. Amer. Hlth. Org. Scient. Publ., (254): 59-64, 1972.

[4] 4. CADAVID, D. & RESTREPO, A. Factors associated with Paracoccidioides brasiliensis infection among permanent residents of three endemic areas in Colombia, SA. Epidem. infect., 111: 121-133, 1993.

[5] 5. CAMPOS, E. P.; BERTOLI, C. I. & BARBOSA, K. S. Linfonodo pulmonar na paracoccidioidomicose aguda infantil. (Relato de um caso). Rev. Soc. bras. Med. trop., 25: 195-200, 1992.

[6] 6. CARDOSO, M. F. T. C. Organizaçăo e reorganizaçăo do espaço do Paraíba do Sul. Uma análise geográfica até 1940. Rev. bras. Geogr., 58: 81-135, 1991.

[7] 7. FORJAZ, M. H. H. Estudo epidemiológico da paracoccidioidomicose. Rastreamento de áreas endęmicas e de "reservareas" no Brasil através do traçado do perfil migratório-residencial-profissional de pacientes diagnosticados em Săo Paulo. Săo Paulo, 1989. (Tese de Doutorado - Escola Paulista de Medicina).

[8] 8. FREITAS, N. A. Paracoccidioidomicose. Inquérito com paracoccidioidina e busca ativa de casos no Município de Campos, RJ. Rio de Janeiro, 1992. (Dissertaçăo de Mestrado - Universidade Federal do Rio de Janeiro).

[9] 9. GIRALDO, R.; RESTREPO, A.; GUTIERREZ, F. et al. Pathogenesis of paracoccidioidomycosis: a model based on the study of 46 patients. Mycopathologia (Den Haag), 58: 63-70, 1976.

[10] 10. LONDERO, A. T.; RAMOS, C. D. & LOPES, J. O. "Reservarea" de paracoccidioidomicose no Rio Grande do Sul, Brasil. Rev. Inst. Med. trop. S. Paulo, 14: 377-380, 1972.

[11] 11. MARTINS, R. M.; MARQUES, J. L. O.; GARCEZ, I. C. S. et al. Paracoccidioidomicose. Relato de caso em criança de tręs anos de idade com a forma disseminada. J. Pediat., 61: 319-322, 1986.

[12] 12. PEDROSA, P. N. Paracoccidioidomicose. Inquérito intradérmico com paracoccidioidina em zona rural do Estado do Rio de Janeiro. Rio de Janeiro, 1976. (Dissertaçăo de Mestrado - Universidade Federal do Rio de Janeiro).

[13] 13. RESTREPO, A. The ecology of Paracoccidioides brasiliensis A puzzle still unsolved. J. med. vet. Mycol., 23: 323-334, 1985.

[14] 14. RIOS-GONÇALVES, A. J.; TERRA, G. M. F.; ROZENBAUM, R. et al. Paracoccidiodomicose em criança de tręs anos de idade. Apresentaçăo de dois casos. Arq. bras. Med., 70: 73-79, 1996.

[15] 15. ROSARIO-FILHO, N. A.; QUEIROZ-TELLES Fo, F.; COSTA, O. et al. Paracoccidioidomycosis in children with different skeletal involvement. Rev. Inst. Med. trop. S. Paulo, 27: 337-340, 1985.

[16] 16. VERAS, K. N. Paracoccidioidomicose. Estudo epidemiológico e clínico de pacientes internados no Hospital de Doenças Infecto Contagiosas em Teresina, Piauí. Identificaçăo de reservareas no Estado do Pará e Maranhăo. Teresina, 1995. (Dissertaçăo de Mestrado - Instituto Oswaldo Cruz, FIOCRUZ, RJ).