Carcaças de frango prontas para consumo como fonte de infecção entérica pelo Campylobacter jejuni, no Brasil

Chicken carcasses as a source of Campylobacter jejuni in Belo Horizonte, Brazil

Tânia C. Dias Dulciene M. M. Queiroz Edilberto N. Mendes José N. Peres Sobre os autores

Resumos

C. jejuni foi pesquisado em carcaças de frango prontas para consumo e em fezes de magarefes de 9 abatedouros não industriais e 1 industrial, tendo sido isolado em 19 (38,0%) dentre 50 carcaças e em 2 (13,3%) dentre 15 amostras de fezes provenientes dos abatedouros não industriais e em 1 (2,0%) dentre 50 carcaças do abatedouro industrializado. Neste último, o microrganismo não foi isolado de nenhuma das 40 amostras de fezes examinadas. O perfil eletroforético em gel de poliacrilamida, as características bioquímicas e o padrão de susceptibilidade aos antimicrobianos apresentados pelas amostras isoladas das carcaças foram muito semelhantes aos das amostras isoladas das fezes de magarefes, o que sugere que as galinhas podem ser a fonte de C. jejuni para os magarefes e que ambos podem estar envolvidos na transmissão do microorganismo.


The presence of Campylobacter jejuni was investigated in stool specimens from chicken meat workers and in ready-for-market chicken carcasses from one industrial and nine non industrial slaughters in Belo Horizonte. In the latter C. jejuni was isolated from 19 (38.0%) of the 50 chicken carcasses and from 2 (13.3%) of the stool specimens obtained from 15 chicken meat workers. In the industrial slaughter it was found in only 1 (2.0%) of the 50 chicken carcasses and it was not isolated from any of the 40 stool specimens. There was a significant difference between industrial and non industrial slaughter in regard to the frequence of C. jejuni isolation from carcasses (p = 0.000002), probably due to the low hygiene conditions present in non industrial slaughters. The results of antimicrobial susceptibility tests, SDS gel electrophoresis and biotyping of the strains isolated from stool specimens obtained from chicken meat workers were similar to those observed in strains isolated from chicken carcasses which suggest that chicken could be the source of C. jejuni for the workers and both, chicken and workers, could be implicated in the transmission of C. jejuni infection in Belo Horizonte.

Campylobacter jejuni; Chicken carcasses; Slaughters; Campylobacter jejuni transmission


ORIGINAL ARTICLES

Chicken carcasses as a source of Campylobacter jejuni in Belo Horizonte, Brazil

Carcaças de frango prontas para consumo como fonte de infecção entérica pelo Campylobacter jejuni, no Brasil

Tânia C. Dias; Dulciene M. M. Queiroz; Edilberto N. Mendes; José N. Peres

Laboratory of Research in Bacteriology — Faculdade de Medicina/UFMG — Av. Alfredo Balena, 190 — Sala 464. CEP 30130 — Belo Horizonte, Minas Gerais, Brazil

SUMMARY

The presence of Campylobacter jejuni was investigated in stool specimens from chicken meat workers and in ready-for-market chicken carcasses from one industrial and nine non industrial slaughters in Belo Horizonte. In the latter C. jejuni was isolated from 19 (38.0%) of the 50 chicken carcasses and from 2 (13.3%) of the stool specimens obtained from 15 chicken meat workers. In the industrial slaughter it was found in only 1 (2.0%) of the 50 chicken carcasses and it was not isolated from any of the 40 stool specimens. There was a significant difference between industrial and non industrial slaughter in regard to the frequence of C. jejuni isolation from carcasses (p = 0.000002), probably due to the low hygiene conditions present in non industrial slaughters. The results of antimicrobial susceptibility tests, SDS gel electrophoresis and biotyping of the strains isolated from stool specimens obtained from chicken meat workers were similar to those observed in strains isolated from chicken carcasses which suggest that chicken could be the source of C. jejuni for the workers and both, chicken and workers, could be implicated in the transmission of C. jejuni infection in Belo Horizonte.

Key words: Campylobacter jejuni; Chicken carcasses; Slaughters; Campylobacter jejuni transmission.

RESUMO

C. jejuni foi pesquisado em carcaças de frango prontas para consumo e em fezes de magarefes de 9 abatedouros não industriais e 1 industrial, tendo sido isolado em 19 (38,0%) dentre 50 carcaças e em 2 (13,3%) dentre 15 amostras de fezes provenientes dos abatedouros não industriais e em 1 (2,0%) dentre 50 carcaças do abatedouro industrializado. Neste último, o microrganismo não foi isolado de nenhuma das 40 amostras de fezes examinadas. O perfil eletroforético em gel de poliacrilamida, as características bioquímicas e o padrão de susceptibilidade aos antimicrobianos apresentados pelas amostras isoladas das carcaças foram muito semelhantes aos das amostras isoladas das fezes de magarefes, o que sugere que as galinhas podem ser a fonte de C. jejuni para os magarefes e que ambos podem estar envolvidos na transmissão do microorganismo.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

Recebido para publicação em 16/3/1990.

  • 1. BLASER, M.J. Campylobacter jejuni and food. Food Technol., 36: 89-92, 1982.
  • 2. BLASER, M.J.; LA FORCE, F.M.; WILSON, N.A. & WANG, W.L.L. Reservoirs for human campylobacteriosis. J. infect. Dis., 141: 665-669, 1980.
  • 3. BLASER, M.J.; WALDMAN, R.J.; BARRET, T. & ERLANDSON, A.L. Outbreaks of Campylobacter enteritis in two extended families: evidence for person-to-person transmission. J. Pediat., 98: 254-257, 1981.
  • 4. BUTZLER, J.P. & SKIRROW, M.B. Campylobacter enteritis. Clin. Gastroent., 8: 737-765, 1979.
  • 5. CHAN, F.T.H. & MACKENZIE, M.R. Enrichment medium and control system for isolation of Campylobacter fetus subsp. jejuni from stools. J. clin. Microbiol., 15: 12-15, 1982.
  • 6. GEORGE, H.A.; HOFFMAN, P.S.; SMIBERT, R.M. & KRIEG, N.R. Improved media for growth and aerotolerance of Campylobacter fetus. J. clin. Microbiol., 8: 36-41, 1978.
  • 7. GRANT, I.H.; RICHARDSON, N.J. & BOKKENHEUSER, V.D. Broiler chickens as potential source of Campylobacter infections in humans. J. clin. Microbiol., 11: 508-510, 1980.
  • 8. HEBERT, G.A.; HOLLIS, D.G.; WEAVER, R.E.; LAMBERT, M.A.; BLASER, M.J. & MOSS, C.W. 30 years of campylobacters: biochemical characteristics and a biotyping proposal for Campylobacter jejuni. J. clin. Microbiol., 15: 1065-1073, 1982.
  • 9. HOPKINS, R.S. & SCOTT, A.S. Handling raw chicken as a source for sporadic Campylobacter jejuni infection. J. infect. Dis., 148: 770, 1979.
  • 10. LAEMMLI, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685, 1970.
  • 11. MENDES, E.N.; QUEIROZ, D.M.M.; CISALPINO, E.O.; PERES, J.N.; PENNA, F.J. & FIGUEIREDO FILHO, P.P. Ocorręncia de Campylobacter jejuni em crianças com e sem diarréia, em Belo Horizonte. Rev. Microbiol. (S. Paulo), 18: 25-30, 1987.
  • 12. SIMMONS, N.A. & GIBBS, F.J. Campylobacter ssp in an owen-ready poultry. J. Infect., 1: 159-162, 1979.
  • 13. SKIRROW, M.B. Campylobacter enteritis: a new disease. Brit. med. J., 2: 9-11, 1987.
  • 14. SKIRROW, M.B. & BENJAMIN, J. "1001" campylobacters: cultural characteristics of intestinal campylobacters from man and animals. J. Hyg. (Lond.), 85: 427-442, 1980.
  • 15. SKIRROW, M.B.; FIDOE, R.G. & JONES, D.M. An outbreak of presumptive food-borne Campylobacter enteritis. J. Infect., 3: 234-236, 1981.
  • 16. STERN, N.J. - Methods for recovery of Campylobacter jejuni from foods. J. Food Protect., 45: 1332-1337, 1982.
  • 17. SVEDHEM, A. & KAIJSER, B. Isolation of Campylobacter jejuni from domestic animals and pets: probable origin of human infection. J. Infect, 3: 37-40, 1981.
  • 18. TAYLOR, D.E.; HARGREAVES, J.A.; NG, L.K.; SHERBANIUK, R.W. & JEWELL, L.D. Isolation and characterization of Campylobacter pyloridis from gastric biopsies. Amer. J. clin. Path., 87: 49-54, 1987.
  • 19. TEE, W.; KALDOR, J. & DWYER, B. Epidemiology of Campylobacter diarrhoeae. Med. J. Aust., 145: 499-503, 1986.

Datas de Publicação

  • Publicação nesta coleção
    11 Set 2006
  • Data do Fascículo
    Dez 1990

Histórico

  • Recebido
    16 Mar 1990
Instituto de Medicina Tropical de São Paulo Av. Dr. Enéas de Carvalho Aguiar, 470, 05403-000 - São Paulo - SP - Brazil, Tel. +55 11 3061-7005 - São Paulo - SP - Brazil
E-mail: revimtsp@usp.br