Monoclonal antibody to serotype 17 of Neisseria meningitidis and their prevalence in Brazilian States

Sacchi, Claudio Tavares; Lemos, Ana Paula Silva de; Gorla, Maria Cecília Outeiro; Frasch, Carl E.

doi:10.1590/S0036-46651995000100001

Abstracts

Neisseria meningitidis are gram-negative diplococci responsible for cases of meningococcal disease all over the world. The epidemic potential of N. meningitidis serogroup B and C is clearly a function of their serotype antigens more than of their capsular polysaccharides. Until recently, hiperimmune sera were used to detect typing antigens on the bacteria. The advent of monoclonal antibodies (MAbs) offered the opportunity to eliminate many of the cross-reactions and have improved the accuracy and reproducibility of meningococcal serotyping. We have produced a MAb to the outer membrane protein of the already existent serotype 17 that have been detected by the use of hiperimmune rabbit sera. The prevalence of this serotype epitope is low in the Brazilian strains. By using the MAb 17 we could not decrease the percentage of nontypeable serogroup C strains. However, there were a decreasing in nontypeable strains to 13% into serogroup B strains and to 25% into the other serogroups.

N. meningitidis B typing

Neisseria meningitidis são diplococos Gram negativos responsáveis por casos de doença meningocócica em todo o mundo. O potencial epidêmico de N. meningitidis sorogrupos B e C é claramente mais uma função de seus antígenos de sorotipo que de seu polissacáride capsular. Até recentemente soros hiperimune foram usados para detectar antígenos de sorotipo em bactérias. O advento de anticorpos monoclonais ofereceu a oportunidade de eliminar muitas das reações cruzadas e têm melhorado a acuracidade e reprodutibilidade da sorotipagem de meningococo. Nós produzimos um anticorpo monoclonal contra proteína de membrana externa do sorotipo 17 que até então tem sido detectado através do uso de soro policlonal. A prevalência deste epítopo de sorotipo é baixa nas cepas brasileiras. Usando-se este anticorpo monoclonal em cepas brasileiras, não pudemos diminuir a porcentagem de cepas sorogrupo C não tipáveis, entretanto, houve uma diminuição de 13% em cepas sorogrupo B não tipáveis e 25% em cepas de outros sorogrupos.

MICROBIOLOGY

Monoclonal antibody to serotype 17 of Neisseria meningitidis and their prevalence in Brazilian States

Anticorpo monoclonal contra o sorotipo 17 de Neisseria meningitidis e sua prevalência em estados brasileiros

Claudio Tavares Sacchi^I; Ana Paula Silva de Lemos^I; Maria Cecília Outeiro Gorla^I; Carl E. Frasch^II

^IDepartment of Health, Adolfo Lutz Institute, Bacteriology Division, 01246-902, São Paulo, Brazil

^IICenter for Epidemiological Evaluation and Research, Bethesda, Maryland 20892

^{Correspondence to} Correspondence to: Claudio T. Sacchi Instituto Adolfo Lutz, Seção de Bacteriologia Av. Dr. Arnaldo 351 01246-902 - S.P., Brazil Phone: 55(011) 851 0111. Fax: 55(011) 851 3505 Electronic mail address: IN% " ALUTZ@Eu. ANSP. BR"

SUMMARY

Neisseria meningitidis are gram-negative diplococci responsible for cases of meningococcal disease all over the world. The epidemic potential of N. meningitidis serogroup B and C is clearly a function of their serotype antigens more than of their capsular polysaccharides. Until recently, hiperimmune sera were used to detect typing antigens on the bacteria. The advent of monoclonal antibodies (MAbs) offered the opportunity to eliminate many of the cross-reactions and have improved the accuracy and reproducibility of meningococcal serotyping. We have produced a MAb to the outer membrane protein of the already existent serotype 17 that have been detected by the use of hiperimmune rabbit sera. The prevalence of this serotype epitope is low in the Brazilian strains. By using the MAb 17 we could not decrease the percentage of nontypeable serogroup C strains. However, there were a decreasing in nontypeable strains to 13% into serogroup B strains and to 25% into the other serogroups.

Keywords:N. meningitidis B typing.

RESUMO

Neisseria meningitidis são diplococos Gram negativos responsáveis por casos de doença meningocócica em todo o mundo. O potencial epidêmico de N. meningitidis sorogrupos B e C é claramente mais uma função de seus antígenos de sorotipo que de seu polissacáride capsular. Até recentemente soros hiperimune foram usados para detectar antígenos de sorotipo em bactérias. O advento de anticorpos monoclonais ofereceu a oportunidade de eliminar muitas das reações cruzadas e têm melhorado a acuracidade e reprodutibilidade da sorotipagem de meningococo. Nós produzimos um anticorpo monoclonal contra proteína de membrana externa do sorotipo 17 que até então tem sido detectado através do uso de soro policlonal. A prevalência deste epítopo de sorotipo é baixa nas cepas brasileiras. Usando-se este anticorpo monoclonal em cepas brasileiras, não pudemos diminuir a porcentagem de cepas sorogrupo C não tipáveis, entretanto, houve uma diminuição de 13% em cepas sorogrupo B não tipáveis e 25% em cepas de outros sorogrupos.

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Recebido para publicação em 20/06/1994.

Aceito para publicação em 25/07/1994.

1. ASHTON, F.E.; RYAN, A.; DIENA, R. & JENNINGS, H.J. - A new serogroup (L) of Neisseria meningitidis. J. clin. Microbiol., 17: 722-727, 1983.
2. FRASCH, C.E. & GOTSCHLICH, E.C. - An outer membrane protein of Neisseria meningitidis group B responsible for serotype specificity. J. exp. Med., 140: 87-104, 1974.
3. FRASCH, C.E.; ZOLLINGER, W.D. & POOLMAN, J.T. - Serotype antigens of Neisseria meningitidis and a proposed scheme for designation of serotypes. Rev. infect. Dis., 7: 504-510, 1985.
4. FRASCH, C.E. - Development of meningococcal serotyping. In: VEDROS, N.A., ed. Evolution of meningococcal disease. Boca Raton, CRC Press, 1987. p.39-54.
5. GOLDSCHNEIDER, I.; GOTSCHLICH, E.C. & GOTSCHLICH, M.S. - Human immunity to the meningococcus. I. The role of humoral antibodies. J. exp. Med., 129: 1307-1326, 1969.
6. HARLOW, E. & LANE, D. - Antibodies. A laboratory manual. New York, Cold Spring Harbor Laboratory, 1988. p. 139-318.
7. HØIBY, E.A.; ROSENQVIST, E.; FRØHOLM, L.O. et al. - Bactericidal antibodies after vaccination with the Norwegian meningococcal serogroup B outer membrane vesicle vaccine: a brief survey. Nat. Inst. Publ. Hlth. Ann., 14: 147-473, 1991.
8. LAEMMLI, U.K. - Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature (Lond.), 227: 680-685, 1970.
9. PELTOLA, H. - Meningococcal disease: still with us. Rev. infect. Dis., 5: 71-91, 1983.
10. POOLMAN, J.T.; HOPMAN, C.T.P. & ZANEN, H.C. - Problems in the definition of meningococcal serotypes. FEMS Microbiol. Lett., 13: 339-348, 1982.
11. SACCHI, C.T.; PESSOA, L.L.; RAMOS, S.R. et al. - Ongoing group B Neisseria meningitidis epidemic in Săo Paulo, Brazil, due to increased prevalence of a single clone of ET-5 complex. J. clin. Micrbiol.,30: 1734-1738, 1992.
12. SHAO-QING, D.; REN-BANG, Y. & HAN-CHUN, Z. - Three new serogroups of Neisseria meningitidis. J. biol. Stand., 9: 307-315, 1972.
13. SUGASAWARA, R.J. - The use of monoclonal antibodies for detecting and serotyping Neisseria meningitidis. In: SUGASAWARA, R.J. Monoclonal antibodies against bacteria. New York, Academic Press, 1985. v.2, p.61-79.
14. TSAI, C.M.; FRASCH, C.E. & MOCCA, L.F. - Five structural classes of major outer membrane proteins in Neisseria meningitidis. J. Bact., 146: 69-78, 1981.
15. WEDEGE, E.; HØIBY, E.A.; ROSENQVIST, E. & FRØHOLM, L.O. - Serotyping and subtyping of Neisseria meningitidis isolates by co-agglutination, dot-blotting and ELISA. J. med. Microbiol., 31: 195-201, 1990.

Correspondence to:

Claudio T. Sacchi

Instituto Adolfo Lutz, Seção de Bacteriologia

Av. Dr. Arnaldo 351

01246-902 - S.P., Brazil

Phone: 55(011) 851 0111. Fax: 55(011) 851 3505

Electronic mail address: IN% "

ALUTZ@Eu. ANSP. BR"

Publication Dates

Publication in this collection
22 Feb 2007
Date of issue
Feb 1995

History

Accepted
25 July 1994
Received
20 June 1994

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ASHTON, F.E.; RYAN, A.; DIENA, R. & JENNINGS, H.J. - A new serogroup (L) of Neisseria meningitidis. J. clin. Microbiol., 17: 722-727, 1983.

[2] 2. FRASCH, C.E. & GOTSCHLICH, E.C. - An outer membrane protein of Neisseria meningitidis group B responsible for serotype specificity. J. exp. Med., 140: 87-104, 1974.

[3] 3. FRASCH, C.E.; ZOLLINGER, W.D. & POOLMAN, J.T. - Serotype antigens of Neisseria meningitidis and a proposed scheme for designation of serotypes. Rev. infect. Dis., 7: 504-510, 1985.

[4] 4. FRASCH, C.E. - Development of meningococcal serotyping. In: VEDROS, N.A., ed. Evolution of meningococcal disease. Boca Raton, CRC Press, 1987. p.39-54.

[5] 5. GOLDSCHNEIDER, I.; GOTSCHLICH, E.C. & GOTSCHLICH, M.S. - Human immunity to the meningococcus. I. The role of humoral antibodies. J. exp. Med., 129: 1307-1326, 1969.

[6] 6. HARLOW, E. & LANE, D. - Antibodies. A laboratory manual. New York, Cold Spring Harbor Laboratory, 1988. p. 139-318.

[7] 7. HØIBY, E.A.; ROSENQVIST, E.; FRØHOLM, L.O. et al. - Bactericidal antibodies after vaccination with the Norwegian meningococcal serogroup B outer membrane vesicle vaccine: a brief survey. Nat. Inst. Publ. Hlth. Ann., 14: 147-473, 1991.

[8] 8. LAEMMLI, U.K. - Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature (Lond.), 227: 680-685, 1970.

[9] 9. PELTOLA, H. - Meningococcal disease: still with us. Rev. infect. Dis., 5: 71-91, 1983.

[10] 10. POOLMAN, J.T.; HOPMAN, C.T.P. & ZANEN, H.C. - Problems in the definition of meningococcal serotypes. FEMS Microbiol. Lett., 13: 339-348, 1982.

[11] 11. SACCHI, C.T.; PESSOA, L.L.; RAMOS, S.R. et al. - Ongoing group B Neisseria meningitidis epidemic in Săo Paulo, Brazil, due to increased prevalence of a single clone of ET-5 complex. J. clin. Micrbiol.,30: 1734-1738, 1992.

[12] 12. SHAO-QING, D.; REN-BANG, Y. & HAN-CHUN, Z. - Three new serogroups of Neisseria meningitidis. J. biol. Stand., 9: 307-315, 1972.

[13] 13. SUGASAWARA, R.J. - The use of monoclonal antibodies for detecting and serotyping Neisseria meningitidis. In: SUGASAWARA, R.J. Monoclonal antibodies against bacteria. New York, Academic Press, 1985. v.2, p.61-79.

[14] 14. TSAI, C.M.; FRASCH, C.E. & MOCCA, L.F. - Five structural classes of major outer membrane proteins in Neisseria meningitidis. J. Bact., 146: 69-78, 1981.

[15] 15. WEDEGE, E.; HØIBY, E.A.; ROSENQVIST, E. & FRØHOLM, L.O. - Serotyping and subtyping of Neisseria meningitidis isolates by co-agglutination, dot-blotting and ELISA. J. med. Microbiol., 31: 195-201, 1990.

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