S. saprophyticus has been frequently isolated from urinary tract infections in young women. In contrast with S. aureus, no defined virulence factors have been recognized for the coagulase negative Staphylococcus species. The objective this study was to analyze the adherence of S. saprophyticus to HEp-2 cells and sheep erythrocytes. The sample were isolated from urine of patients with urinary infection. Hemagglutination, adherence to HEp-2 cells tests and inhibition by specific carbohydrates of the interactions between these cells were analyzed. Most of the strains were hemagglutinanting whose properties was inhibited by mannose (100mM). There was a high adherence level to HEp-2 cells. The differences in specificity and attachment level noted in this study suggest that multiple adhesins are involved in the mechanism of cellular interaction.