Frequency and distribution of HPV genotypes in 800 genital samples of Ecuadorian men and women from the city of Guayaquil

ABSTRACT

Even though there are data published on HPV epidemiology in Ecuador, the distribution of genotypes in Guayaquil, the largest city in the country, has not been previously determined in a study including including both, men and women. The present study aimed to determine the distribution of 37 HPV genotypes in genital samples from Ecuadorian men and women living in the city of Guayaquil. Genital samples included in daily diagnostic routine were analyzed by the 37 HPV GenoArray Diagnostic Kit (Hybribio^® Ltd., Sheung Wan, Hong Kong). The relative frequency of detectable genotypes was determined. HPV relative frequency according to sample characteristics, including sex and age groups, was compared using c² test. From the 800 samples (400 men and 400 women), 411 (51.38%) were positive for HPV DNA. The obtained frequency was higher among samples from men (253/400 or 63.25%) in comparison to samples from women (158/400 or 39.50%), with a p value <0.05. Samples from men showed a higher frequency of HPV genotypes 6, 16, 18 and 11, while among samples from women genotypes 39, 16, 6 and 58 were the most frequent. Considering male and female samples together, genotypes 6, 16, 39 and 11 presented the highest frequencies. HPV DNA was detected in half of the studied samples, with a higher frequency among samples from men. Genotype 39 was the most frequent among women, and ranked third when samples from men and women are analyzed together.

HPV; Ecuador; Males and females; HPV genotypes

INTRODUCTION

Human Papillomavirus (HPV) genital infection, constitutes the most common sexually transmitted disease worldwide¹ . HPV is considered a highly contagious pathogen, transmitted by sexual contact, by contact with the skin or through inanimate objects² - ⁶ . According to the International Agency for Research in Cancer (IARC) and the World Health Organization (WHO), 12 HPV genotypes, namely 16, 18, 31, 33, 35, 39, 45 51, 52, 56, 58 and 59, are considered high risk genoypes and belong to the group I of human biologic carcinogens. HPV 68 is classified as probably carcinogenic and genotypes 26, 30, 34, 53, 66, 67, 69, 70, 73, 82 and 85 are classified as possibly carcinogenic; these genotypes correspond to groups 2A and 2B, respectively⁷ , ⁸ . In previous studies on HPV epidemiology in Ecuador, many authors have identified a number of genotypes that were not HPV16 and HPV18⁹ - ¹³ .

The bivalent vaccine (HPV genotypes 16 and 18), which offers cross protection for some HPV genotypes of the same group as HPV16 (HPV31, HPV33, HPV35, HPV52, HPV58) and HPV18 (HPV39, HPV45, HPV59, HPV68), is funded by the government¹⁴ - ¹⁶ . As the frequency of genotypes other than HPV16 and 18 is still not clear in Ecuador, the present study aimed to determine the relative frequency and distribution of HPV genotypes in 800 genital samples (400 obtained from men and 400 obtained from women), included in the daily diagnostic routine of the Instituto Oncológico Nacional de la Sociedad de Lucha Contra el Cáncer ( ION SOLCA Matriz), in Guayaquil, Ecuador, which is responsible for the care of 50% to 70% of all cancers, in the country. The data obtained will provide guidance for prevention strategies, mainly of vaccination.

MATERIALS AND METHODS

Clinical samples

From January to December 2018 a cross sectional study was carried out in the Instituto Oncologico Nacional de la Sociedad de Lucha Contra el Cancer , in Guayaquil, Ecuador. During this period, a total of 800 genital brushings were obtained according to medical indication, from 400 men and 400 women aged 10-70 years old. Samples were consecutively enrolled in the study. Genital samples were collected by using the Hybribio^® PCR Cell Collection Media at the outpatient service SOLCA, from January 2015 until December 2018. The collected samples were finally processed at SOLCA’s Molecular Biology Laboratory, which is a reference laboratory in the city.

DNA extraction

DNA extraction was performed following the methodology recommended by the manufacturer.

HPV genotyping

HPV genotypes were identified using the 37 HPV GenoArray Diagnostic Kit (Hybribio^® Diagnostics Ltd., Sheung Wan, Hong Kong) that identify the following 37 HPV genotypes: 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 26, 34, 40, 54, 55, 57, 61, 67, 69, 70, 71, 72, 73, 82, 83, 84, 6, 11, 42, 43, 44 and 81 (CP8304). Following the protocol provided by the manufacturer, a conventional PCR using the PGMY set of primers was performed (Applied Biosystems^®/Gene Amp^® 9700 thermal cycler). Immediately after amplification, the PCR product was denatured and hybridized through specific probes for 20 min. Additional steps included an initial washing, aggregation of the conjugate, blocking of the hybridization, secondary washing procedures and the addition of the substrate. This last step triggers the appearance of a specific spot thanks to a color reaction that occurs in the presence of the corresponding HPV genotype.

The quality and the sufficiency of DNA samples for the PCR assays were evaluated by amplifying an internal control, that was included in the PCR mix. The absence of the internal control amplification constituted the samples exclusion criterium, even though the internal control of the dot blot had worked. This control was present in all the routines.

Data analysis

Results were considered positive if specific dot blots for any investigated HPV genotype and the internal control were present. The frequency and distribution of HPV genotypes was determined. HPV frequency according to sample characteristics (sex and age groups), was compared using c² test. There was no need to obtain the informed consent from participants as the data were retrieved from daily HPV genotyping routines. This study was not evaluated by ethics committees due to the hospital’s internal policies.

RESULTS

From the total of 800 samples (400 from men and 400 from women), 411 were positive for HPV with an overall frequency of 51.38%. The frequency for men samples (253/400 or 63.25%) was higher than that of women samples (158/400 or 39.50%), with a p value <0.05. Comparing other characteristics, such as the detection of one or multiple HPV genotypes according to sex, or the frequency of HPV genotypes in different age groups, was not statistically significant with p values >0.05 ( Table 1 ).

Table 1
Frequency of HPV-positive or –negative samples according to sex, age group and presence of one HPV genotype or multiple genotypes.

Variables	HPV+	HPV–	p value X2 test
Sex
Men	253	147	< 0.05*
Women	158	242
Age (years)
18-29	144	120	>0.05
30-39	131	153
40-49	83	73
50-59	34	34
60-70	19	9
Variables	One genotype	Multiple genotypes	p value X2 test
Sex
Men	157	96	>0.05
Women	86	72

• *Statistically significant

The most common viral genotype including both, men and women samples, was HPV 6, with 110 cases (relative frequency of 26.76%), followed by HPV 16, with 67 cases (relative frequency of 16.30%), HPV 39 with 43 cases (relative frequency of 10.46%) and HPV 11 with 35 cases (relative frequency of 8.52%). HPV 18 and HPV 58 were the following genotypes with 33 and 31 cases, respectively. Genotypes detected in women samples were: HPV 39 (17.09%), HPV 16 (13.92%), HPV 6 (13.29%) and HPV 58 (10.76%). Genotypes detected in men samples were: HPV 6 (35.18%), HPV 16 (17.39%), HPV 18 (10.67%) and HPV 11 (10.28%) ( Figure 1 ). Overall, genotype 66, ranked seventh in frequency. Among female samples, this genotype exhibited a slightly higher frequency (8.86% vs 5.93%) ( Figure 1 ). From the 411 positive samples, in 168 multiple genotypes were detected: 93 had two genotypes, 39 had three genotypes and 36 had more than three genotypes.

Figure 1
Frequency of different human papillomavirus genotypes in men (depicted in black) and in women (depicted in white).

CONCLUSION

Human papillomavirus infection is one of the most prevalent viral sexually transmitted disease¹⁷ . Approximately 291 million women are harboring HPV viruses at any time in life, with 23% of these infections related to HPV 16 and 8.5% to HPV 18¹⁸ . The adjusted global prevalence has been reported as 10.41%, with regional differences in the frequency of carcinogenic HPV infections¹ . In the case of heterosexually identified men, an overall prevalence of 50.5% was reported in the Study on Human Papillomavirus Infection in Men, that included participants from Brazil, Mexico and the United States¹⁹ . Worldwide, 570.000 cancer cases per year in women and 60.000 cancer cases in men are attributable to HPV, representing 8.6% and 0.8% of all cancer cases. In the particular case of cervical cancer, infection rates standardized according to age that are over 30 per 100.000 are reported mainly in sub-Saharan Africa and some places in Latin America and Oceania²⁰ .

To our knowledge, HPV has not been studied in both men and women in the city of Guayaquil, Ecuador. Most of the samples were sent to the laboratory with little or no information on the participants’ clinical conditions, previous diagnosis or treatment, besides gender and age. This lack of information was the main limitation of the study. The HPV frequency found in women samples (39.50%) and reported here were similar to the one from a previous observational study, using similar methodology but with a larger sample size of 1,581 Ecuadorian women (43.58%). We noticed, however, that some HPV genotypes associated with cancer, including HPV 39 and HPV 58, appeared to be more frequent according to the locality²¹ . A Peruvian study has also indicated an analogous frequency of HPV infection (43.4%), in a group of female college students²² . On the contrary, a Colombian study reported a slightly higher frequency of HPV infection in female adolescents (48.97%)²³ . In the case of men, the frequency reported in this study (63.25%) was higher than the one reported for heterosexual men (50%)²⁴ . Furthermore, we observed that male samples showed a higher proportion of infection by HPV genotypes covered by currently used vaccines. This finding supports the need of to include men in vaccination strategies. As mentioned by other authors, only bivalent and tetravalent vaccines have been available for vaccination in Ecuador²⁵ - ²⁷ . Due to the circulation of genotypes different from the ones covered by the available vaccines, a broader immunization strategy should be considered.

ACKNOWLEDGEMENTS

We acknowledge our technical personnel at the Molecular Biology Department.

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• ERRATUM
  Page 1, where it reads:
  Gustavo David García Muentes, Marcia Adelaida Mendoza García, Ramiro Israel Burgos Galárraga, Kleber Ollague, Cynthia Vargas Wachter, Juan Carlos Ruiz Cabezas
  Should be read:
  Gustavo David García-Muentes, Marcia Adelaida Mendoza-García, Ramiro Israel Burgos-Galárraga, Kleber Ollague, Cynthia Vargas-Wachter, Juan Carlos Ruiz-Cabezas
  Rev Inst Med Trop Sao Paulo. 2019;61:e41
  http://dx.doi.org/10.1590/s1678-9946201961041

Publication Dates

• Publication in this collection
  19 Aug 2019
• Date of issue
  2019

History

• Received
  15 Jan 2019
• Accepted
  16 July 2019