Experimental pulmonary schistosomiasis: lack of morphological evidence of modulation in schistosomal pulmonary granulomas

Esquistossomose pulmonar experimental: falta de evidência morfológica de modulação nos granulomas esquistosomóticos pulmonares

Maura R. F. Souza Vidal Aryon A. Barbosa Jr. Zilton A. Andrade About the authors

Abstracts

Numerous pulmonary schistosome egg granulomas were present in mice submitted to partial portal vein ligation (Warren's model). The granulomas were characterized by cellular aggregations formed within alveolar tissue. Main cellular types were macrophages (epithelioid cells), eosinophils, plasma cells and lymphocytes. These cells were supported by scanty fibrous stroma and exhibited close membrane contact points amongst themselves, but without forming specialized adhesion apparatus. When granulomas involved arterial structures, proliferation of cndothelial and smooth muscle cells occurred and fibrosis associated with angiogenesis became more evident. Granulomas formed around mature eggs in the pulmonary alveolar tissue presented approximately the same size and morphology regardless of the time of infection, the latter being 10, 18 and 25 weeks after cercarial exposure. This persistence of morphological appearance suggests that pulmonary granulomas do not undergo immunological modulation, as is the case with the granulomas in the liver and, to a lesser extent, in the intestines. Probably, besides general immunological factors, local (stromal) factors play an important role in schistosomal granuloma modulation.

Experimental schistosomiasis; Pulmonary schistosomiasis; Periovular granuloma; Immunological modulation


Em camundongos submetidos à ligadura parcial da veia porta e infecção pelo Schistosoma mansoni, os granulomas periovulares apareceram em grande número nos pulmões, comprovando a validade do modelo de Warren. Histologicamente os granulomas eram representados por agregados celulares compactos no seio de escasso estroma. Os macrófagos (células epitelióides) e eosinófilos eram os elementos celulares predominantes, vindo em seguida os linfóticos e plasmócitos. Ultraestruturalmenle, as células do granuloma exibiam íntimo contacto de suas membranas, com varios pontos de adesão, mas sem formar estruturas juncionais mais específicas. Os granulomas formados em torno a ovos maduros tinham tamanho, forma e composição celular similares após 10, 18 ou 25 semanas de infecção. Este aspecto contrastava com o que aparecia nos granulomas hepáticos os quais exibiam a clássica modulação. Os granulomas que envolviam as paredes arteriolares exibiam proliferação muscular, endotelial, com formação de novas vias vasculares e fibrose. As diferenças morfológicas observadas nos granulomas formados em diferentes locais e órgãos apontam para a importância de se considerar fatores locais (estromais) no determinismo dos padrões das respostas inflamatórias.


ORIGINAL ARTICLES

Experimental pulmonary schistosomiasis:* * This paper is an abridged version of a Thesis for the Master's degree in Pathology presented to the Federal University of Bahia by Maura Raquel Ferreira Souza Vidal. lack of morphological evidence of modulation in schistosomal pulmonary granulomas

Esquistossomose pulmonar experimental: falta de evidência morfológica de modulação nos granulomas esquistosomóticos pulmonares

Maura R. F. Souza Vidal; Aryon A. Barbosa Jr.; Zilton A. Andrade

Centro de Pesquisas Gonçalo Moniz (FIOCRUZ). Rua Valdemar Falcão, 121, 40.295-001 Salvador, Bahia - Brazil

Address for correspondence

SUMMARY

Numerous pulmonary schistosome egg granulomas were present in mice submitted to partial portal vein ligation (Warren's model). The granulomas were characterized by cellular aggregations formed within alveolar tissue. Main cellular types were macrophages (epithelioid cells), eosinophils, plasma cells and lymphocytes. These cells were supported by scanty fibrous stroma and exhibited close membrane contact points amongst themselves, but without forming specialized adhesion apparatus. When granulomas involved arterial structures, proliferation of cndothelial and smooth muscle cells occurred and fibrosis associated with angiogenesis became more evident.

Granulomas formed around mature eggs in the pulmonary alveolar tissue presented approximately the same size and morphology regardless of the time of infection, the latter being 10, 18 and 25 weeks after cercarial exposure. This persistence of morphological appearance suggests that pulmonary granulomas do not undergo immunological modulation, as is the case with the granulomas in the liver and, to a lesser extent, in the intestines. Probably, besides general immunological factors, local (stromal) factors play an important role in schistosomal granuloma modulation.

Key words: Experimental schistosomiasis; Pulmonary schistosomiasis; Periovular granuloma; Immunological modulation.

RESUMO

Em camundongos submetidos à ligadura parcial da veia porta e infecção pelo Schistosoma mansoni, os granulomas periovulares apareceram em grande número nos pulmões, comprovando a validade do modelo de Warren. Histologicamente os granulomas eram representados por agregados celulares compactos no seio de escasso estroma. Os macrófagos (células epitelióides) e eosinófilos eram os elementos celulares predominantes, vindo em seguida os linfóticos e plasmócitos. Ultraestruturalmenle, as células do granuloma exibiam íntimo contacto de suas membranas, com varios pontos de adesão, mas sem formar estruturas juncionais mais específicas. Os granulomas formados em torno a ovos maduros tinham tamanho, forma e composição celular similares após 10, 18 ou 25 semanas de infecção. Este aspecto contrastava com o que aparecia nos granulomas hepáticos os quais exibiam a clássica modulação. Os granulomas que envolviam as paredes arteriolares exibiam proliferação muscular, endotelial, com formação de novas vias vasculares e fibrose.

As diferenças morfológicas observadas nos granulomas formados em diferentes locais e órgãos apontam para a importância de se considerar fatores locais (estromais) no determinismo dos padrões das respostas inflamatórias.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

ACKNOWLEDGEMENT

We thank Dr. Eilson Goes Oliveira for his invaluable assistance with the statistical analysis.

  • Address for correspondence:
    Zilton A. Andrade,. M.D.
    Centro de Pesquisas Gonçalo Moniz (FIOCRUZ)
    Rua Valdemar Falcão, 121
    40.295-001 Salvador, Bahia - Brazil
    Telephone: (55.71) 359-4320; Fax: (55.71)359-4292
  • Recebido para publicação em 14/04/1993.

    Aceito para publicação em 29/06/1993.

  • *
    This paper is an abridged version of a Thesis for the Master's degree in Pathology presented to the Federal University of Bahia by Maura Raquel Ferreira Souza Vidal.
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    Address for correspondence: Zilton A. Andrade,. M.D. Centro de Pesquisas Gonçalo Moniz (FIOCRUZ) Rua Valdemar Falcão, 121 40.295-001 Salvador, Bahia - Brazil Telephone: (55.71) 359-4320; Fax: (55.71)359-4292 * This paper is an abridged version of a Thesis for the Master's degree in Pathology presented to the Federal University of Bahia by Maura Raquel Ferreira Souza Vidal.

    Publication Dates

    • Publication in this collection
      14 July 2006
    • Date of issue
      Oct 1993

    History

    • Received
      14 Apr 1993
    • Accepted
      29 June 1993
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