Immunomodulatory effect of glucan on the response to experimental antirabies vaccination

Tino, Milene S.; Carrieri, Maria L.; Zanetti, Carlos R; Mendes, Nelson F.; Pereira, Octavio A.C.

doi:10.1590/S0036-46651993000500008

Abstracts

The objective of lhe present study was to determine the stimulatory response to antirabies vaccination promoted by glucan in mice. Glucan increased both resistance to infection and antibody titres and this effect was more evident when glucan was used at dose of 0.5 mg, administered intraperitoneally before, during and after immunization and when the challenge virus was applied to the foot-pad.

Glucan; Antirabies vaccination; Rabies

No presente trabalho verificou-se a atividade estimuladora da resposta à vacinação anti-rábica experimental determinada pela glucana em camundongos. Esta atividade pôde ser detectada por aumento da resistência à infecção e pela resposta imune mais intensa em termos de títulos de anticorpos. Estes resultados foram mais evidentes quando a glucana foi utilizada em doses de 0.5mg, administrada por via i.p., antes, durante e após a imunização e o desafio feito no coxim plantar da pata posterior.

IMMUNOLOGY

Immunomodulatory effect of glucan on the response to experimental antirabies vaccination

Efeito imunomodulatório da glucana na resposta à vacinação anti-rábica experimental

Milene S. Tino^I; Maria L. Carrieri^II; Carlos R Zanetti^II; Nelson F. Mendes^I; Octavio A.C. Pereira^II

^IDisciplina de Imunologia da Escola Paulista de Medicina

^IIInstituto Pasteur, São Paulo

^{Correspondence to} Correspondence to: Milene S. Tino Avenida Paulista, 393 CEP - 01311-000 São Paulo, SP. Brasil

SUMMARY

The objective of lhe present study was to determine the stimulatory response to antirabies vaccination promoted by glucan in mice. Glucan increased both resistance to infection and antibody titres and this effect was more evident when glucan was used at dose of 0.5 mg, administered intraperitoneally before, during and after immunization and when the challenge virus was applied to the foot-pad.

Key words: Glucan; Antirabies vaccination; Rabies.

RESUMO

No presente trabalho verificou-se a atividade estimuladora da resposta à vacinação anti-rábica experimental determinada pela glucana em camundongos. Esta atividade pôde ser detectada por aumento da resistência à infecção e pela resposta imune mais intensa em termos de títulos de anticorpos. Estes resultados foram mais evidentes quando a glucana foi utilizada em doses de 0.5mg, administrada por via i.p., antes, durante e após a imunização e o desafio feito no coxim plantar da pata posterior.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

ACKNOWLEDGEMENTS

The authors gratefully acknowledge Mrs. Dolores Ayako Yoda, Mrs. Maria Aparecida da Silva and Mr. Jose Luiz de Carvalho Jr. for the technical assistence. This work was supported by The Health Secretary of the State of São Paulo, CNPq and FINEP.

Recebido para publicação em 15/4/1993.

Aceito para publicação em 6/5/1993.

1. BROWDER, W.; McNAMEE, R.B. & DI LUZIO, N.R. - Glucan Ąmmunotherapy as adjunct in the surgical excision of experimental melanoma. Surg. Forum, 28: 171-173, 1977.
2. CELIS, E.; WIKTOR, T.; DIETZSCITOLD, B. & KOPROWSKJ, H. - Amplification of rabies virus-induced stimulation of human T-cell lines and clones by antigen-specific antibody. J. Virol., 56: 426-433, 1985.
3. COOK, J.A.; TAYLOR, D.; COHEN, C.; HOFFMANN, E.O.; RODRIGUES, J.; MALSHET, V. & DI LUZIO, N.R. - Evaluation of effector cells mediating the antitumor action of glucan. J. reliculoendoth. Soc., 22: 21-34, 1977.
4. DEIMANN, W. & FAHIMI, W. - Induction of focal hemopoiesis in adult rat liver by glucan, a macrophage activador. A cytochemical and ultrastructural study. Lab. Invest., 42: 217-224, 1980.
5. DELVILLE, J. & JACQUES, V.J. - Therapeutic effect of intravenously administered yeast glucan in mice locally infected by Mycobacterium leprae. Advanc. exp. Med. Biol., 121: 245-253, 1980.
6. DI LUZIO, N.R.; McNAMEE, R.B.; WILLIAMS, D.L.; GILBERT, K.M. & SPANGERS, M.A. - Glucan induced inhibition of tumor growth and enhancement of survival in a variety of transplantable and spontaneous murine tumor models. Advanc. exp. Med. Biol., 121: 269-290, 1980.
7. DI LUZIO, N.R.; PISANO, J.C. & SABA, T.M. - Evaluation of the mechanism of glucan induced stimulation of the reticuloendothelial system. J. reticuluendoth. Soc, 7: 731-742, 1970.
8. FUENZALIDA, E. & PALÁCIOS, R. - Un método mejorado en la preparación de la vacuna antirábica. Bol. Inst. bact. Chile, 8: 3-10, 1955.
9. HASSID, W.Z.; JOSLYN, M.A. & McCREADY, R.M. - The molecular constitution of an Ąnsolute polysaccharide from yeast Saccharomyces cerevisiae. J. Amer. Chem. Soc., 63: 295-298, 1941.
10. HOLBROOK, T.W.; COOK, J.A. & PARKER, B.W. - Immunization against Leishmania donovani: glucan as an adjuvant with killed promastigotes. Amer. J. trop. Med. Hyg., 30: 762-768, 1981.
11. KOKOSHIS, P.L.; WILLIAMS D.L.; COOK, J.A. & DI LUZIO, N.R. - Increased resistance to Staphylococcus aureus infection and enhancement in serum lysozyme activity by glucan. Science, 199: 1340-1342, 1978.
12. KOPROWSKI, H.; MOCARELLI, P. & WIKTOR, T. - Antibody responses in vitro to an animal virus: production of rabies virus neutralizing antibodies by mouse cells in culture. Proc. nat. Acad. Sci. (Wash.), 69: 2433-2436, 1972.
13. REED, L.J. & MUENCH, H. - A simple method of estimating fifty percent endpoints. Amer. J. Hyg., 27: 493-497, 1938.
14. REYNOLDS, J.A.; KASTELO, M.D.; HARRINGTON, D.G.; CRABBS, C.L.; PETERS, C.J.; JEMSKI, J.V.; SCOTT, G.H. & DI LUZIO, N.R. - Glucan-induced enhancement of host resistance to selected infectious diseases. Infect. Immun., 30: 51-57, 1980.
15. SELIGMANN, E.B. - Prueba de potencia NIH. In: KAPLAN, M.M. & KOPROWSKI, H. La Rabia: técnicas de laboratório. 3a. ed. Ginebra, Organización Mundial de la Salud, 1976. p. 294-302.
16. SIEGEL, S. - Estatística năo paramétrica para as cięncias do comportamento. Săo Paulo, McGraw Hill do Brasil, 1975.
17. TURNER, G. & BALLARD, R. - Interaction of mouse peritoneal macrophages with fixed rabies virus in vivo and in vitro. J. gen. Virol., 30: 223-231, 1976.
18. WILLIAMS, D.L.; BROWDER, W. & DI LUZIO N.R. - Immunotherapeutic modification of Escherichia coli - induced experimental peritonitis and bacteremia by glucan. Surgery, 93: 448-454, 1983.
19. WILLIAMS, D.L.; COOK, J.A.; HOFFMAN, E.O. & DI LUZIO, N.R. - Protective effect of glucan in experimentally induced candidiasis. J. reticuloendoth. Soc., 23: 479-490, 1978.
20. WILLIAMS, D.L. & DI LUZIO, N.R. - Glucan induced modification of murine viral hepatitis. Science, 208: 67-69, 1980.
21. WOOLES, W.R. & DI LUZIO, N.R. - Reticuloendothelial function and the immune response. Science, 142: 1078-1080, 1963.
22. WOOLES, W.R. & DI LUZIO, N.R. - The phagocytic and proliferative response of the reticuloendothelial system following glucan administration. J. reticuloendoth. Soc., 1: 160-169, 1964.
23. ZANETTI, C.R.; TINO M.S.; CHAMELET, E.L.B. & PEREIRA, O.A.C. - Simplification of adherence hemagglutination test for detection of rabies antibodies in human serum. Rev. Inst. Med. trop. S. Paulo, 31: 341-345, 1989.

Correspondence to:

Milene S. Tino

Avenida Paulista, 393

CEP - 01311-000 São Paulo, SP. Brasil

Publication Dates

Publication in this collection
04 July 2006
Date of issue
Oct 1993

History

Accepted
06 May 1993
Received
15 Apr 1993

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BROWDER, W.; McNAMEE, R.B. & DI LUZIO, N.R. - Glucan Ąmmunotherapy as adjunct in the surgical excision of experimental melanoma. Surg. Forum, 28: 171-173, 1977.

[2] 2. CELIS, E.; WIKTOR, T.; DIETZSCITOLD, B. & KOPROWSKJ, H. - Amplification of rabies virus-induced stimulation of human T-cell lines and clones by antigen-specific antibody. J. Virol., 56: 426-433, 1985.

[3] 3. COOK, J.A.; TAYLOR, D.; COHEN, C.; HOFFMANN, E.O.; RODRIGUES, J.; MALSHET, V. & DI LUZIO, N.R. - Evaluation of effector cells mediating the antitumor action of glucan. J. reliculoendoth. Soc., 22: 21-34, 1977.

[4] 4. DEIMANN, W. & FAHIMI, W. - Induction of focal hemopoiesis in adult rat liver by glucan, a macrophage activador. A cytochemical and ultrastructural study. Lab. Invest., 42: 217-224, 1980.

[5] 5. DELVILLE, J. & JACQUES, V.J. - Therapeutic effect of intravenously administered yeast glucan in mice locally infected by Mycobacterium leprae. Advanc. exp. Med. Biol., 121: 245-253, 1980.

[6] 6. DI LUZIO, N.R.; McNAMEE, R.B.; WILLIAMS, D.L.; GILBERT, K.M. & SPANGERS, M.A. - Glucan induced inhibition of tumor growth and enhancement of survival in a variety of transplantable and spontaneous murine tumor models. Advanc. exp. Med. Biol., 121: 269-290, 1980.

[7] 7. DI LUZIO, N.R.; PISANO, J.C. & SABA, T.M. - Evaluation of the mechanism of glucan induced stimulation of the reticuloendothelial system. J. reticuluendoth. Soc, 7: 731-742, 1970.

[8] 8. FUENZALIDA, E. & PALÁCIOS, R. - Un método mejorado en la preparación de la vacuna antirábica. Bol. Inst. bact. Chile, 8: 3-10, 1955.

[9] 9. HASSID, W.Z.; JOSLYN, M.A. & McCREADY, R.M. - The molecular constitution of an Ąnsolute polysaccharide from yeast Saccharomyces cerevisiae. J. Amer. Chem. Soc., 63: 295-298, 1941.

[10] 10. HOLBROOK, T.W.; COOK, J.A. & PARKER, B.W. - Immunization against Leishmania donovani: glucan as an adjuvant with killed promastigotes. Amer. J. trop. Med. Hyg., 30: 762-768, 1981.

[11] 11. KOKOSHIS, P.L.; WILLIAMS D.L.; COOK, J.A. & DI LUZIO, N.R. - Increased resistance to Staphylococcus aureus infection and enhancement in serum lysozyme activity by glucan. Science, 199: 1340-1342, 1978.

[12] 12. KOPROWSKI, H.; MOCARELLI, P. & WIKTOR, T. - Antibody responses in vitro to an animal virus: production of rabies virus neutralizing antibodies by mouse cells in culture. Proc. nat. Acad. Sci. (Wash.), 69: 2433-2436, 1972.

[13] 13. REED, L.J. & MUENCH, H. - A simple method of estimating fifty percent endpoints. Amer. J. Hyg., 27: 493-497, 1938.

[14] 14. REYNOLDS, J.A.; KASTELO, M.D.; HARRINGTON, D.G.; CRABBS, C.L.; PETERS, C.J.; JEMSKI, J.V.; SCOTT, G.H. & DI LUZIO, N.R. - Glucan-induced enhancement of host resistance to selected infectious diseases. Infect. Immun., 30: 51-57, 1980.

[15] 15. SELIGMANN, E.B. - Prueba de potencia NIH. In: KAPLAN, M.M. & KOPROWSKI, H. La Rabia: técnicas de laboratório. 3a. ed. Ginebra, Organización Mundial de la Salud, 1976. p. 294-302.

[16] 16. SIEGEL, S. - Estatística năo paramétrica para as cięncias do comportamento. Săo Paulo, McGraw Hill do Brasil, 1975.

[17] 17. TURNER, G. & BALLARD, R. - Interaction of mouse peritoneal macrophages with fixed rabies virus in vivo and in vitro. J. gen. Virol., 30: 223-231, 1976.

[18] 18. WILLIAMS, D.L.; BROWDER, W. & DI LUZIO N.R. - Immunotherapeutic modification of Escherichia coli - induced experimental peritonitis and bacteremia by glucan. Surgery, 93: 448-454, 1983.

[19] 19. WILLIAMS, D.L.; COOK, J.A.; HOFFMAN, E.O. & DI LUZIO, N.R. - Protective effect of glucan in experimentally induced candidiasis. J. reticuloendoth. Soc., 23: 479-490, 1978.

[20] 20. WILLIAMS, D.L. & DI LUZIO, N.R. - Glucan induced modification of murine viral hepatitis. Science, 208: 67-69, 1980.

[21] 21. WOOLES, W.R. & DI LUZIO, N.R. - Reticuloendothelial function and the immune response. Science, 142: 1078-1080, 1963.

[22] 22. WOOLES, W.R. & DI LUZIO, N.R. - The phagocytic and proliferative response of the reticuloendothelial system following glucan administration. J. reticuloendoth. Soc., 1: 160-169, 1964.

[23] 23. ZANETTI, C.R.; TINO M.S.; CHAMELET, E.L.B. & PEREIRA, O.A.C. - Simplification of adherence hemagglutination test for detection of rabies antibodies in human serum. Rev. Inst. Med. trop. S. Paulo, 31: 341-345, 1989.