Histological and immunohistochemical aspects of cutaneous leishmaniasis in nicaragua

Aspectos histológicos e imunohistoquírnicos da leishmaniose cutânea na Nicarágua

Abstracts

Sixty skin biopsies of fifty-eight non-treated patients with cutaneous leishmaniasis from Nicaragua were studied. Biopsies were classified according to Ridley's histopathologic groups: 36 (60%) were included in group B (diffuse necrosis), 14 in group C (focal necrosis), 7 (11.6%) in group D (reactive tuberculoid). Only three biopsies remained unclassified. None of our cases could be included in groups A and E. Immunohistochemistry disclosed in 17 cases Russel's bodies secreting IgM and only one IgG positive. There was no correlation between the histopathologic groups and the presence of Russel's bodies. However, we believe the presence of Russel's bodies help the diagnosis of leishmaniasis. Correlation between morphological patterns and the different leishmania species was also considered.

Cutaneous leishmaniasis; Nicaragua; Pathology; Histological classification; Immunohistochemistry; Russel's bodies


Foram estudadas 60 biopsias cutâneas de 58 pacientes nicaraguenses com leishmaniose tegumentar e ainda não em tratamento terapêutico. Quase todas as biopsias foram classificadas nos grupos histopatológicos de Ridley: 36 (60%) foram incluídas no grupo B ("diffuse necrosis"), 14 (23%) no grupo C ("focal necrosis"), 7 (11.6%) no grupo D ("reactive tuberculoid"). Somente 3 biopsias ficaram sem classificação. Não se achou nenhum caso que se pudesse classificar nos grupos A e E. Em 18 casos, usando método imunohis-toquímico, foram observados corpúsculos de Russel. Destes 18 casos 17 eram IgM positivos e só 1 IgG positivo. Não se descobriu correlação entre os grupos histopatológicos e a presença de corpúsculos de Russel. Todavia se imagina que os corpúsculos de Russel possam ter um significado diagnóstico (como um critério adjunto). Pode-se supor também uma correlação entre as numerosas alterações morfológicas e as diferentes espécies de leishmanias.


ORIGINAL ARTICLE

Histological and immunohistochemical aspects of cutaneous leishmaniasis in nicaragua

Aspectos histológicos e imunohistoquírnicos da leishmaniose cutânea na Nicarágua

Cesare MariscottiI; Mattia BarbareschiI; Eduardo MissoniII; Rosella MorelliIII; Uriel GuevaraIV; Juan Munguia-BarreraIV

I3rd. Chair of Morbid Anatomy and Histopathology, University of Milan, Italy

IIHealth Center "Fidel Ventura", Waslala, Republic of Nicaragua

IIIDepartment of Infectious Diseases, City Hospital "L. Sacco", Milan, Italy

IVDepartment of Pathology, Leon University, Nicaragua

Address for correspondence

SUMMARY

Sixty skin biopsies of fifty-eight non-treated patients with cutaneous leishmaniasis from Nicaragua were studied. Biopsies were classified according to Ridley's histopathologic groups: 36 (60%) were included in group B (diffuse necrosis), 14 in group C (focal necrosis), 7 (11.6%) in group D (reactive tuberculoid). Only three biopsies remained unclassified.

None of our cases could be included in groups A and E.

Immunohistochemistry disclosed in 17 cases Russel's bodies secreting IgM and only one IgG positive. There was no correlation between the histopathologic groups and the presence of Russel's bodies. However, we believe the presence of Russel's bodies help the diagnosis of leishmaniasis. Correlation between morphological patterns and the different leishmania species was also considered.

Key words: Cutaneous leishmaniasis - Nicaragua; Pathology; Histological classification; Immunohistochemistry; Russel's bodies.

RESUMO

Foram estudadas 60 biopsias cutâneas de 58 pacientes nicaraguenses com leishmaniose tegumentar e ainda não em tratamento terapêutico.

Quase todas as biopsias foram classificadas nos grupos histopatológicos de Ridley: 36 (60%) foram incluídas no grupo B ("diffuse necrosis"), 14 (23%) no grupo C ("focal necrosis"), 7 (11.6%) no grupo D ("reactive tuberculoid"). Somente 3 biopsias ficaram sem classificação.

Não se achou nenhum caso que se pudesse classificar nos grupos A e E.

Em 18 casos, usando método imunohis-toquímico, foram observados corpúsculos de Russel. Destes 18 casos 17 eram IgM positivos e só 1 IgG positivo.

Não se descobriu correlação entre os grupos histopatológicos e a presença de corpúsculos de Russel.

Todavia se imagina que os corpúsculos de Russel possam ter um significado diagnóstico (como um critério adjunto).

Pode-se supor também uma correlação entre as numerosas alterações morfológicas e as diferentes espécies de leishmanias.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

ACKNOWLEDGEMENTS

We wish to thank Prof. D. S. Ridley, Bland Sutton Institute of Pathology, Middlesex Hospital Medical School, London, for his useful comments and suggestions.

Recebido para publicação em 23/10/86.

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  • Address for correspondence:
    Prof. Cesare Mariscotti.
    IIICattedra di Anatomia ed Istologia Patológica
    Via Commenda 19; I - 20122 Milano, Italy.

Publication Dates

  • Publication in this collection
    17 Feb 2011
  • Date of issue
    Aug 1987

History

  • Received
    23 Oct 1986
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