Immunomodulatory effect of cimetidine on the proliferative responses of splenocytes from T. cruzi-infected rats

Efeito imunomodulatório da cimetidina sobre a resposta blastogênica de esplenócitos de ratos infectados por T. cruzi

Abstracts

The immunomodulatory effect of cimetidine (CIM), a histamine type-2 receptor antagonist, was evaluated in respect to the blastogenic response to Con A of Wistar Furth (WF) rats infected by the Y strain of Trypanosoma cruzi (T. cruzi). Enhancement of blastogenesis of normal splenocytes was observed at a concentration of 10-3M. However, the splenocytes from infected animals responded to concentrations of CIM ranging from 10-8 to 10-3M. The mitogenic response to Con A of cells from infected animals was restored in the presence of CIM. The results show that CIM modulates the "in vitro" proliferative response of cells from T. cruzi-infected rats and suggest an immunoregulatory role of histamine and/or of cells that express H2 receptors in this infection.

Trypanosoma cruzi; Rat; Inbred strain; Cimetidine; Immunoregulation


O efeito imunomodulatório da Cimetidine (CIM), um antagonista do receptor de histamina-tipo 2, foi avaliado na resposta blastogênica a Con A em células de ratos Wistar Furth (WF) infectados pela cepa Y de Trypanosoma cruzi (T. cruzi). Foi observado que apenas na concentração de 10-3M de Cimetidine houve amplificação da resposta blastogênica de esplenócitos normais a Con A. Entretanto, a capacidade mitogênica de esplenócitos de animais infectados foi restaurada na presença de molaridades da droga que variaram entre 10-8 a 10-3. Os resultados demonstraram que a CIM tem o potencial de modular a resposta mitogênica de células de animais infectados pelo T. cruzi, sugerindo um papel imunoregulatório da histamina e/ou células que expressam receptores H2 nesta infecção.


ORIGINAL ARTICLES

Immunomodulatory effect of cimetidine on the proliferative responses of splenocytes from T. cruzi-infected rats

Efeito imunomodulatório da cimetidina sobre a resposta blastogênica de esplenócitos de ratos infectados por T. cruzi

M.N. Sato; E.H. Yamashiro-Kanashiro; M.M. Tanji; R. Kaneno; R.Y. Iqueoka; A.J.S. Duarte

Laboratório de Imunogenética e Transplante Experimental (LIM 73) da Disciplina de Alergia e Imunopatologia da Faculdade de Medicina da Universidade de São Paulo, São Paulo, SP

Address for correspondence

SUMMARY

The immunomodulatory effect of cimetidine (CIM), a histamine type-2 receptor antagonist, was evaluated in respect to the blastogenic response to Con A of Wistar Furth (WF) rats infected by the Y strain of Trypanosoma cruzi (T. cruzi). Enhancement of blastogenesis of normal splenocytes was observed at a concentration of 10-3M. However, the splenocytes from infected animals responded to concentrations of CIM ranging from 10-8 to 10-3M. The mitogenic response to Con A of cells from infected animals was restored in the presence of CIM. The results show that CIM modulates the "in vitro" proliferative response of cells from T. cruzi-infected rats and suggest an immunoregulatory role of histamine and/or of cells that express H2 receptors in this infection.

Key words: Trypanosoma cruzi; Rat; Inbred strain; Cimetidine; Immunoregulation.

RESUMO

O efeito imunomodulatório da Cimetidine (CIM), um antagonista do receptor de histamina-tipo 2, foi avaliado na resposta blastogênica a Con A em células de ratos Wistar Furth (WF) infectados pela cepa Y de Trypanosoma cruzi (T. cruzi). Foi observado que apenas na concentração de 10-3M de Cimetidine houve amplificação da resposta blastogênica de esplenócitos normais a Con A. Entretanto, a capacidade mitogênica de esplenócitos de animais infectados foi restaurada na presença de molaridades da droga que variaram entre 10-8 a 10-3. Os resultados demonstraram que a CIM tem o potencial de modular a resposta mitogênica de células de animais infectados pelo T. cruzi, sugerindo um papel imunoregulatório da histamina e/ou células que expressam receptores H2 nesta infecção.

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ACKNOWLEDGEMENTS

We wish thank Dr. Judith Kloetzel for providing Y strain of T. cruzi, Maria Celia Rezende for maintenance of inbred rats. Edna de Souza Joaquim for providing us with reagents, Maria de Lourdes Moura Antonio for secretarial work, Luiz Augusto Marcondes Fonseca and Dewton Vasconcelos for reviewing this manuscript. Research supported by FINEP (PADCT, nº 43.84.0819.00), CNPq (nº 403749/82) and LIM/HC/FMUSP.

  • Address for correspondence:
    Dr. Alberto J.S. Duarte
    Faculdade de Medicina da Universidade de São Paulo, Laboratório de Imunogenética e Transplante Experimental (LIM-73)
    Av. Dr. Arnaldo, 455 - 2º andar - sala 105A
    CEP 01246 São Paulo, SP. Brazil
  • Recebido para publicação em 19/10/1990

    Aceito para publicação em 16/4/1991

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    Address for correspondence: Dr. Alberto J.S. Duarte Faculdade de Medicina da Universidade de São Paulo, Laboratório de Imunogenética e Transplante Experimental (LIM-73) Av. Dr. Arnaldo, 455 - 2º andar - sala 105A CEP 01246 São Paulo, SP. Brazil

    Publication Dates

    • Publication in this collection
      12 Sept 2006
    • Date of issue
      June 1991

    History

    • Accepted
      16 Apr 1991
    • Received
      19 Oct 1990
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