Host cell adhesion to Schistosoma mansoni larvae in the peritoneal cavity of naive mice: histological and scanning electron microscopic studies

Melo, Alan Lane de; Machado, Conceição Ribeiro da Silva; Pereira, Leógenes Horácio

doi:10.1590/S0036-46651993000100003

Abstracts

Cercariae of Schistosoma mansoni inoculated into the peritoneal cavity of naive mice induced host cell adhesion to their surface, but after 90 minutes the number of adherent cells sharply decreased. The cell detachment is progressive and simultaneous to the cercaria-schistosomule transformation. The histological study showed mainly neutrophils in close contact with the larvae. Mononuclear cells and some eosinophils were occasionally seen surrounding the adherent neutrophils. The scanning electron microscopy showed cells displaying twisted microvilli and several microplicae contacting or spreading over the larval surface, and larvae completely surrounded by clusters of cells. These results suggest that the neutrophils recognize molecules on the cercarial surface which induce their spreading

Schistosoma mansoni; Cell adhesion; Neutrophils; Cercaria

A inoculação de cercárias de Schistosoma mansoni na cavidade peritoneal de camundongos normais induz uma aderência de células do hospedeiro a essas larvas. Essa adesão decresce rapidamente quando a larva infectante transforma-se em esquistossômulo. O destacamento das células é progressivo e simultâneo à transformação. Os métodos histológicos e a microscopia eletrônica de varredura mostraram que o neutrófilo é a célula predominante em estreito contacto com a larva. Células mononucleadas e eosinófilos foram observados rodeando o parasito, usualmente sem estar em contacto direto com a larva. Os resultados indicam que neutrófilos podem reconhecer, na superfície larvária, moléculas que induzem sua adesão e espalhamento.

ORIGINAL ARTICLES

Host cell adhesion to Schistosoma mansoni larvae in the peritoneal cavity of naive mice. Histological and scanning electron microscopic studies

Adesão celular às larvas de Schistosoma mansoni na cavidade peritoneal de camundongos normais. Estudos histológicos e microscopia eletrônica de varredura

Alan Lane de Melo^I; Conceição Ribeiro da Silva Machado^II; Leógenes Horácio Pereira^I

^IDepartamento de Parasitologia do Instituto de Ciências Biológicas da UFMG

^IIDepartamento de Morfologia do Instituto de Ciências Biológicas da UFMG

^{Address for correspondence} Address for correspondence: Prof. Dr. Alan Lane de Melo Departamento de Parasitologia do Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais Cx. P.2486 CEP 30161-970 Belo Horizonte - MG, Brasil

SUMMARY

Cercariae of Schistosoma mansoni inoculated into the peritoneal cavity of naive mice induced host cell adhesion to their surface, but after 90 minutes the number of adherent cells sharply decreased. The cell detachment is progressive and simultaneous to the cercaria-schistosomule transformation. The histological study showed mainly neutrophils in close contact with the larvae. Mononuclear cells and some eosinophils were occasionally seen surrounding the adherent neutrophils. The scanning electron microscopy showed cells displaying twisted microvilli and several microplicae contacting or spreading over the larval surface, and larvae completely surrounded by clusters of cells.

These results suggest that the neutrophils recognize molecules on the cercarial surface which induce their spreading.

Key words: Schistosoma mansoni; Cell adhesion; Neutrophils; Cercaria

RESUMO

A inoculação de cercárias de Schistosoma mansoni na cavidade peritoneal de camundongos normais induz uma aderência de células do hospedeiro a essas larvas. Essa adesão decresce rapidamente quando a larva infectante transforma-se em esquistossômulo. O destacamento das células é progressivo e simultâneo à transformação. Os métodos histológicos e a microscopia eletrônica de varredura mostraram que o neutrófilo é a célula predominante em estreito contacto com a larva. Células mononucleadas e eosinófilos foram observados rodeando o parasito, usualmente sem estar em contacto direto com a larva. Os resultados indicam que neutrófilos podem reconhecer, na superfície larvária, moléculas que induzem sua adesão e espalhamento.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

Recebido para publicação em 30/04/1992

Aceito para publicação em 02/12/1992

Supported by The Parasitic Diseases Programme - WHO, Switzerland, CNPq, FINEP, and FAPEMIG, Brazil.

1. BENTLEY, A.G.; CARLISLE, A.S. & PHILLIPS, S.M. - Ultrastructural analysis of the cellular response to Schistosoma mansoni II - Inflammatory responses in rodent skin. Amer. J. trop. Med. Hyg., 30: 815-824, 1981.
2. BLOCH, E.H. - In vivo microscopy of schistosomiasis. IV - Dynamics of the host parasite responses to Schistosoma mansoni in the hypodermic tissues as observed in transparent chambers in two susceptible hosts during primary and challenge infections. Amer. J. trop. Med. Hyg., 33: 899-910, 1984.
3. BUTTERWORTH, A.E. - Cell mediated damage to helminths. Advanc. Parasit., 23: 143-235, 1984.
4. CAULFIELD, J.P.; KORMAN, G.; BUTTERWORTH, A.E.; KOOGAN, M. & DAVID, J.R. - The adherence of human neutrophils and eosinophils to schistosomula: evidence for membrane fusion between cells and parasites. J. Cell Biol., 86: 46-63, 1980.
5. CAULFIELD, J.P.; KORMAN, G. & SAMUELSON, J.C. - Human neutrophils endocytose multivalent ligands from the surface of schistosomula of Schistosoma mansoni before membrane fusion. J. Cell Biol., 94: 370-378, 1982.
6. COUSIN, C.C.; STTREWALT, M.A.; DORSEY, C.H. & WATSON, O.L.P.- Schistosoma mansoni: comparative development of schistosomules produced by artificial techniques. J. Parasit, 72: 606-609, 1986.
7. CRAM, E.B. & BOZICEVICH, J. - Experimental Schistosoma mansoni infection by intraperitoneal injection. Trop. Med. News, 1: 16-17, 1944.
8. EVELAND, L.K. - Schistosoma mansoni: conversion of cercariae to schistosomula. Exp. Parasit., 32: 261-264, 1972.
9. INCANI, R.N. & McLAREN, D.J. - Neutrophil-mediated citotoxicity to schistosomula of Schistosoma mansoni in vitro: studies on the kinetics of complement and/or antibody-dependent adherence and killing. Paras. Immunol., 3: 107-126, 1981.
10. INCANI, R.N. & McLAREN, D.J. - Ultrastructural observations on the in vitro interactions of rat neutrophils with schistosomula of Schistosoma mansoni in the presence of antibody and/or complement. Parasitology, 86: 345-357, 1983.
11. INCANI, R.N. & McLAREN, D.J. - Histopathological and ultrastructural studies of cutaneous reactions elicited in naive and chronically infected mice by invading schistosomules of Schistosoma mansoni. Int. J. Parasit., 14: 259-276, 1984.
12. MELO, A.L.; MACHADO, C.R.S. & PEREIRA, L.H. - Differences between in vitro and in vivo obtained schistosomules. Rev. Inst. Med. trop. S. Paulo, 32: 84-85, 1990.
13. MELO, A.L. & PEREIRA, L.H. - Effect of oxamniquine on the detachment of the tail of Schistosoma mansoni cercaria. J. Parasit, 66: 1067-1068, 1980.
14. MELO, A.L. & PEREIRA, L.H. - Kinetics of the cercaria-schistosomulum transformation, in vivo. Rev. Soc. bras. Med. trop., 18: 17-21, 1985.
15. MELO, A.L. & PEREIRA, L.H. - Kinetics of the cercaria-schistosomulum transfonnation, in vivo: 2- The effect of oxamniquine. Rev. Soc. bras. Med. trop., 18: 251-255, 1985.
16. MELO, A.L.; PEREIRA, L.H. & CORREA, M.C.R. - In vivo cercaria-schistosomulum transformation: inhibitory effect of oxamniquine. Trans. roy. Soc. trop. Med. Hyg., 72: 158-159, 1978.
17. PELLEGRINO, J. & MACEDO, D.G. - A simplified method for the concentration of cercariae. J. Parasit, 41: 329-330, 1955.
18. PEARCE, E.J. & McLAREN, D.J. - Schistosoma mansoni: the cutaneous response to cercarial challenge in naive guinea pigs and guinea pigs vaccinated with highly irradiated cercariae. Int. J. Parasit., 16: 491-510, 1986.
19. PEREIRA, L.H.; PELLEGRINO, J.; VALADARES, T.E.; MELLO, R.T. & COELHO, P.M.Z. - A new approach for screening prophylactic agents in schistosomiasis. Rev. Inst. Med. trop. S. Paulo, 16: 123-126, 1974.
20. SAMUELSON, J.C.; SHER, A. & CAULFIELD, J.P. - Newly transformed schistosomula spontaneously lose surface antigens and C3 acceptor sites during culture. J. Immunol., 124: 2055-2057, 1980.
21. SAVAGE, A.M. & COLLEY, D.G. - The eosinophil in the inflammatory response to cercarial challenge of sensitized and chronically infected CBA/J mice. Amer. J. trop. Med. Hyg., 29: 1268-1278, 1980.
22. STIREWALT, M.A. - Schistosoma mansoni; cercaria to schistosomule. Advanc. Parasit., 12: 115-182, 1974.
23. VON LICHTENBERG, F.; SHER, A.; GIBBONS, N. & DOUGHTY, B.L. - Eosinophil enriched inflammatory response to Schistosoma in the inflammatory response to schistosomula in the skin of mice immune to Schistosoma mansoni. Amer. J. Path., 84: 479-500, 1976.
24. YOLLES, T.K.; MOORE, D.V. & MELENEY, H.E. - Post cercarial development of Schistosoma mansoni in the rabbit and hamster after intraperitoneal and percutaneous infection. J. Parasit., 35: 276-294, 1949.

Address for correspondence:

Prof. Dr. Alan Lane de Melo

Departamento de Parasitologia do Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais

Cx. P.2486

CEP 30161-970 Belo Horizonte - MG, Brasil

Publication Dates

Publication in this collection
11 Sept 2006
Date of issue
Feb 1993

History

Accepted
02 Dec 1992
Received
30 Apr 1992

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BENTLEY, A.G.; CARLISLE, A.S. & PHILLIPS, S.M. - Ultrastructural analysis of the cellular response to Schistosoma mansoni II - Inflammatory responses in rodent skin. Amer. J. trop. Med. Hyg., 30: 815-824, 1981.

[2] 2. BLOCH, E.H. - In vivo microscopy of schistosomiasis. IV - Dynamics of the host parasite responses to Schistosoma mansoni in the hypodermic tissues as observed in transparent chambers in two susceptible hosts during primary and challenge infections. Amer. J. trop. Med. Hyg., 33: 899-910, 1984.

[3] 3. BUTTERWORTH, A.E. - Cell mediated damage to helminths. Advanc. Parasit., 23: 143-235, 1984.

[4] 4. CAULFIELD, J.P.; KORMAN, G.; BUTTERWORTH, A.E.; KOOGAN, M. & DAVID, J.R. - The adherence of human neutrophils and eosinophils to schistosomula: evidence for membrane fusion between cells and parasites. J. Cell Biol., 86: 46-63, 1980.

[5] 5. CAULFIELD, J.P.; KORMAN, G. & SAMUELSON, J.C. - Human neutrophils endocytose multivalent ligands from the surface of schistosomula of Schistosoma mansoni before membrane fusion. J. Cell Biol., 94: 370-378, 1982.

[6] 6. COUSIN, C.C.; STTREWALT, M.A.; DORSEY, C.H. & WATSON, O.L.P.- Schistosoma mansoni: comparative development of schistosomules produced by artificial techniques. J. Parasit, 72: 606-609, 1986.

[7] 7. CRAM, E.B. & BOZICEVICH, J. - Experimental Schistosoma mansoni infection by intraperitoneal injection. Trop. Med. News, 1: 16-17, 1944.

[8] 8. EVELAND, L.K. - Schistosoma mansoni: conversion of cercariae to schistosomula. Exp. Parasit., 32: 261-264, 1972.

[9] 9. INCANI, R.N. & McLAREN, D.J. - Neutrophil-mediated citotoxicity to schistosomula of Schistosoma mansoni in vitro: studies on the kinetics of complement and/or antibody-dependent adherence and killing. Paras. Immunol., 3: 107-126, 1981.

[10] 10. INCANI, R.N. & McLAREN, D.J. - Ultrastructural observations on the in vitro interactions of rat neutrophils with schistosomula of Schistosoma mansoni in the presence of antibody and/or complement. Parasitology, 86: 345-357, 1983.

[11] 11. INCANI, R.N. & McLAREN, D.J. - Histopathological and ultrastructural studies of cutaneous reactions elicited in naive and chronically infected mice by invading schistosomules of Schistosoma mansoni. Int. J. Parasit., 14: 259-276, 1984.

[12] 12. MELO, A.L.; MACHADO, C.R.S. & PEREIRA, L.H. - Differences between in vitro and in vivo obtained schistosomules. Rev. Inst. Med. trop. S. Paulo, 32: 84-85, 1990.

[13] 13. MELO, A.L. & PEREIRA, L.H. - Effect of oxamniquine on the detachment of the tail of Schistosoma mansoni cercaria. J. Parasit, 66: 1067-1068, 1980.

[14] 14. MELO, A.L. & PEREIRA, L.H. - Kinetics of the cercaria-schistosomulum transformation, in vivo. Rev. Soc. bras. Med. trop., 18: 17-21, 1985.

[15] 15. MELO, A.L. & PEREIRA, L.H. - Kinetics of the cercaria-schistosomulum transfonnation, in vivo: 2- The effect of oxamniquine. Rev. Soc. bras. Med. trop., 18: 251-255, 1985.

[16] 16. MELO, A.L.; PEREIRA, L.H. & CORREA, M.C.R. - In vivo cercaria-schistosomulum transformation: inhibitory effect of oxamniquine. Trans. roy. Soc. trop. Med. Hyg., 72: 158-159, 1978.

[17] 17. PELLEGRINO, J. & MACEDO, D.G. - A simplified method for the concentration of cercariae. J. Parasit, 41: 329-330, 1955.

[18] 18. PEARCE, E.J. & McLAREN, D.J. - Schistosoma mansoni: the cutaneous response to cercarial challenge in naive guinea pigs and guinea pigs vaccinated with highly irradiated cercariae. Int. J. Parasit., 16: 491-510, 1986.

[19] 19. PEREIRA, L.H.; PELLEGRINO, J.; VALADARES, T.E.; MELLO, R.T. & COELHO, P.M.Z. - A new approach for screening prophylactic agents in schistosomiasis. Rev. Inst. Med. trop. S. Paulo, 16: 123-126, 1974.

[20] 20. SAMUELSON, J.C.; SHER, A. & CAULFIELD, J.P. - Newly transformed schistosomula spontaneously lose surface antigens and C3 acceptor sites during culture. J. Immunol., 124: 2055-2057, 1980.

[21] 21. SAVAGE, A.M. & COLLEY, D.G. - The eosinophil in the inflammatory response to cercarial challenge of sensitized and chronically infected CBA/J mice. Amer. J. trop. Med. Hyg., 29: 1268-1278, 1980.

[22] 22. STIREWALT, M.A. - Schistosoma mansoni; cercaria to schistosomule. Advanc. Parasit., 12: 115-182, 1974.

[23] 23. VON LICHTENBERG, F.; SHER, A.; GIBBONS, N. & DOUGHTY, B.L. - Eosinophil enriched inflammatory response to Schistosoma in the inflammatory response to schistosomula in the skin of mice immune to Schistosoma mansoni. Amer. J. Path., 84: 479-500, 1976.

[24] 24. YOLLES, T.K.; MOORE, D.V. & MELENEY, H.E. - Post cercarial development of Schistosoma mansoni in the rabbit and hamster after intraperitoneal and percutaneous infection. J. Parasit., 35: 276-294, 1949.

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