Potential infectivity of blood from HBsAG asymptomatic carriers due to the presence of HBV-DNA and comparison with other markers of hbv infection

Potencial de infectividade em sangue de portadores assintomáticos de HBsAg caracterizado pela presença de HBV-DNA e comparação com outros marcadores da Hepatite B.

Abstracts

Serum samples from 356 HBsAg positive asymptomatic carriers, which were titrated by reverse passive hemagglutination, were analysed for the presence of HBV-DNA, HBsAg and IgM anti-HBc. The samples were divided in three classes, according to the titers of HBsAg and IgM anti-HBc and the distribution of HBV-DNA and HBsAg among these classes was studied. In the high titer class of HBsAg, 65% of samples have one or both markers against only 19% in the low titer class. From the total of 356 samples, 121 gave positive results for IgM anti-HBc (33.9%). From these, 38.9% of HBV-DNA and 47.9% of HBeAg were observed, whereas in samples with absence of IgM anti-HBc, 18.3% and 16.6% were respectively found. A higher frequency of agreement between all these markers was found in the class of high titers of HBsAg; however, HBV-DNA was detected in the low titer class of HBsAg and little or no IgM anti-HBc, showing potential blood infectivity even in HBsAg positive borderline samples.

HBV-DNA; HBsAg; IgM anti-HBc Asymptomatic carriers


Em 356 soros HBsAg positivos de portadores assintomáticos, titulados por hemaglutinação passiva reversa, foi analisada a presença de HBV DNA, HBeAg e IgM anti-HBc, da seguinte forma: as amostras foram divididas em três classes de acordo com o título de HBsAg e IgM anti-HBc e foi verificada a distribuição de HBV DNA e HBsAg nestas classes. Em amostras com títulos elevados de HBsAg, 65% das amostras tiveram um ou ambos os marcadores HBV-DNA e HBsAg e as mesmas foram encontradas em apenas 19% das amostras com baixo título de HBsAg. Do total de 356 amostras, 121 foram positivas para IgM anti-HBc (33.9%). Destas, 38.9% de HBV-DNA e 47.9% de HBeAg foram observadas, ao passo que em amostras com ausência de IgM anti-HBc, 18.3% e 16.6% foram respectivamente encontrados. A concordância entre os três marcadores foi encontrada em. amostras com alto título de HBsAg, entretanto HBV-DNA foi também detectado em grupo de baixo título de HBsAg na ausência de IgM anti-HBc ou em níveis baixos, mostrando o potencial de infectividade mesmo em amostras de HBsAg com valores próximos ao limite da positividade.


ORIGINAL ARTICLES

Potential infectivity of blood from HBsAG asymptomatic carriers due to the presence of HBV-DNA and comparison with other markers of hbv infection

Potencial de infectividade em sangue de portadores assintomáticos de HBsAg caracterizado pela presença de HBV-DNA e comparação com outros marcadores da Hepatite B.

M. Valeria TedeschiI; Cláudia Figueiredo PadillaI; Iná Ferraz De CamargoII; Clara F. Tachibana YoshidaI

IDepartamento de Virologia, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil

IIBio-Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil

Address for correspondence

SUMMARY

Serum samples from 356 HBsAg positive asymptomatic carriers, which were titrated by reverse passive hemagglutination, were analysed for the presence of HBV-DNA, HBsAg and IgM anti-HBc. The samples were divided in three classes, according to the titers of HBsAg and IgM anti-HBc and the distribution of HBV-DNA and HBsAg among these classes was studied. In the high titer class of HBsAg, 65% of samples have one or both markers against only 19% in the low titer class. From the total of 356 samples, 121 gave positive results for IgM anti-HBc (33.9%). From these, 38.9% of HBV-DNA and 47.9% of HBeAg were observed, whereas in samples with absence of IgM anti-HBc, 18.3% and 16.6% were respectively found.

A higher frequency of agreement between all these markers was found in the class of high titers of HBsAg; however, HBV-DNA was detected in the low titer class of HBsAg and little or no IgM anti-HBc, showing potential blood infectivity even in HBsAg positive borderline samples.

Key words: HBV-DNA; HBsAg; IgM anti-HBc Asymptomatic carriers.

RESUMO

Em 356 soros HBsAg positivos de portadores assintomáticos, titulados por hemaglutinação passiva reversa, foi analisada a presença de HBV DNA, HBeAg e IgM anti-HBc, da seguinte forma: as amostras foram divididas em três classes de acordo com o título de HBsAg e IgM anti-HBc e foi verificada a distribuição de HBV DNA e HBsAg nestas classes.

Em amostras com títulos elevados de HBsAg, 65% das amostras tiveram um ou ambos os marcadores HBV-DNA e HBsAg e as mesmas foram encontradas em apenas 19% das amostras com baixo título de HBsAg. Do total de 356 amostras, 121 foram positivas para IgM anti-HBc (33.9%). Destas, 38.9% de HBV-DNA e 47.9% de HBeAg foram observadas, ao passo que em amostras com ausência de IgM anti-HBc, 18.3% e 16.6% foram respectivamente encontrados.

A concordância entre os três marcadores foi encontrada em. amostras com alto título de HBsAg, entretanto HBV-DNA foi também detectado em grupo de baixo título de HBsAg na ausência de IgM anti-HBc ou em níveis baixos, mostrando o potencial de infectividade mesmo em amostras de HBsAg com valores próximos ao limite da positividade.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

ACKNOWLEDGEMENTS

The authors gratefully acknowledge Messias da Silva, Luiz Antonio C. Mercadante and Sergio da Silva e Mouta Jr. for technical assistance. Claudia F. Padilla was a trainee in our Department with a fellowship from CNPq.

Part of this work was supported by CNPq, grant number 40.0081.89.0.

Recebido para publicação em 16/5/1989.

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  • Address for correspondence:
    Dra. M. Valeria Tedeschi
    Fundação Oswaldo Cruz
    Departamento de Virologia
    Av. Brasil 4365
    CEP 21040 Rio de Janeiro, RJ, Brazil.

Publication Dates

  • Publication in this collection
    01 Dec 2010
  • Date of issue
    Dec 1989

History

  • Received
    16 May 1989
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