Immunopathology of human schistosomiasis mansoni: I. Immunomodulatory influences on T cell function

Aldrey, O.; Noya, B.; Machado, I.; Noya, O.; Bianco, N. E.; Perez, G. E.

doi:10.1590/S0036-46651988000600001

Abstracts

Cell mediated immune response was studied in patients with recent and chronic Schistosoma mansoni infection. Precultured peripheral mononuclear cells showed significantly higher responses to S. mansoni adult worm antigen (SAWA) when compared to fresh cell preparations. The addition of each patient serum to the precultured cells reactions to SAWA or recall antigens demonstrated a strong inhibitory serum action, which was also noted on allogeneic cells derived from healthy subjects. The CD4 subset was the main responding cell to SAWA being this reactivity highly suppressed by the presence of the monocyte macrophage accessory cells. We stressed the simultaneous inhibitory action of humoral and cellular factors on the specific cell response to S. mansoni.

Immunoregulation; T cells; Human schistosomiasis

A resposta imune celular foi estudada em pacientes com infecção recente ou crônica por Schistosoma mansoni. Células mononucleares do sangue periférico pré cultivadas reagiram significantemente a antígenos do verme adulto (SAWA) do S. mansoni quando comparadas à preparação contendo células frescas. A adição de soro autólogo às células pré-cultivadas resultou em inibição da reação frente a SAWA ou antígenos de memória; o mesmo efeito foi notado quando os soros de pacientes foram adicionados a culturas de células alogênicas obtidas de indivíduos normais. A subpopulação CD4 foi a principal população celular respondedora a SAWA, sendo que esta reatividade foi intensamente suprimida na presença de preparações purificadas contendo monócitos-macrófagos. Estes resultados sugerem a ação de fatores inibidores, tanto humorais como celulares, sobre a resposta imune celular específica ao S. mansoni.

ORIGINAL ARTICLES

Immunopathology of human schistosomiasis mansoni. I. Immunomodulatory influences on T cell function(¹ (1 ) Research supported by grants from CONICTT (Fortalecimiento de Centros) and from CDCH M 09.15 84 (UCV). )

Imunopatologia da esquistossomose mansônica humana. I. Influências imunorregulatórias sobre a função T

O. Aldrey; B. Noya; I. Machado; O. Noya; N. E. Bianco; G. E. Perez

Clinical Immunology National Center, Ministry of Health, WHO PAHO, Collaborative Center for Clinical Immunology and the Tropical Medicine and Pathology Institutes, Central University. Venezuela

^{Address for correspondence} Address for correspondence: Oscar Aldrey, MD Inmunología Clínica Apartado 50647 Correos de Sabana Grande Caracas 1051, Venezuela

SUMMARY

Cell mediated immune response was studied in patients with recent and chronic Schistosoma mansoni infection. Precultured peripheral mononuclear cells showed significantly higher responses to S. mansoni adult worm antigen (SAWA) when compared to fresh cell preparations. The addition of each patient serum to the precultured cells reactions to SAWA or recall antigens demonstrated a strong inhibitory serum action, which was also noted on allogeneic cells derived from healthy subjects. The CD4 subset was the main responding cell to SAWA being this reactivity highly suppressed by the presence of the monocyte macrophage accessory cells. We stressed the simultaneous inhibitory action of humoral and cellular factors on the specific cell response to S. mansoni.

Key words: Immunoregulation; T cells; Human schistosomiasis.

RESUMO

A resposta imune celular foi estudada em pacientes com infecção recente ou crônica por Schistosoma mansoni. Células mononucleares do sangue periférico pré cultivadas reagiram significantemente a antígenos do verme adulto (SAWA) do S. mansoni quando comparadas à preparação contendo células frescas. A adição de soro autólogo às células pré-cultivadas resultou em inibição da reação frente a SAWA ou antígenos de memória; o mesmo efeito foi notado quando os soros de pacientes foram adicionados a culturas de células alogênicas obtidas de indivíduos normais. A subpopulação CD4 foi a principal população celular respondedora a SAWA, sendo que esta reatividade foi intensamente suprimida na presença de preparações purificadas contendo monócitos-macrófagos. Estes resultados sugerem a ação de fatores inibidores, tanto humorais como celulares, sobre a resposta imune celular específica ao S. mansoni.

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ACKNOWLEDGMENT

Research supported by grants from CONICIT (Fortalecimiento de Centros) and from CDCH-M-09.15/84 (UCV).

We thank Amanda Gonzalez and Yajaira Echegaray for the transcription of the manuscript.

Recebido para publicação em 29/07/1987.

1. ALARCON DE NOYA, B.; NOYA, O.; BRITO DE URBAEZ, R. & RISQUEZ, J. - Reactivación de un foco de esquistosomiasis mansoni en Venezuela. Bol. Direcc. Malar., 27(1-4), 1987.
2. ARANGO, M.; OROPEZA, F.; ANDERSON, O.; CONTRERAS, C. E.; BIANCO, N. & YARZABAL, L. - Circulating immune complexes and the "in vitro" cell reactivity in paracoccidioidomycosis. Mycopathologia (Den Haag). 79: 153-158, 1982.
3. BARSOUM, I. S.; GAMIL, F.M.; & RAMZY, R. M. - Immune response and immunoregulation in relation to human schistosomiasis in Egypt. Effect of treatment on in vitro cellular responsiveness. Amer, J. trop. Med. Hyg. 31: 1181-1187, 1982.
4. BOYUM, A. - Isolation of mononuclear cells and granulocytes from human blood. Scand. J. Clin. Lab. Invest., 21 (supp. 97): 77, 1968.
5. CAPRON, M.; DESSAINT, J. P. & JOSEPH, M. - Effectors mechanisms against schistosoma. Progr. Immunol., 5: 1305-1316, 1983.
6. COLLEY, D. G : COOK, J. A. & JORDAN, P. - Immune responses during human schistosomiasis mansoni. I. In vitro lymphocyte blastogenic responses to heteregeneous antigenic preparations from schistosome eggs, worms and cercariae. Int. Arch. Allergy, 53: 420-433. 1979.
7. COLLEY, D. G., HIENY, S E.; BARTHOLOMEW, R. K. & COOK, J. A. - Immune responses during human Schistosomiasis mansoni. III. Regulatory effect of patient sera on human lymphocyte blastogenic responses to schistosome antigen preparations. Amer. J., trop. Med. Hyg., 26: 917-925, 1977.
8. COLLEY, D. G.; LEWIS, F. A. & GOODGAME, R. W. - Immune response during human Schistosomiasis mansoni. IV. Induction of suppressor cell activity by Schistosoma antigens preparations. J. Immunol., 120: 1225-1232, 1978.
9. CONTRERAS, C. E.; OROZCO, A.; SANCHEZ, P.; ORTEGA, G. & BIANCO. N. E. - Physiological aspect of circulating immune complexes in the normal population. Clin. exp. Immunol., 48. 693-699, 1982.
10. DAMIAN, R. - Immune schistosomiasis, a holistic view. Contemp. Top. Immunobiol., 12: 359-420, 1984.
11. GAZZINELLI, G.; LAMBERTUCCI, J. R.; KATZ, N.; ROCHA, R.; LIMA, M. & COLLEY, D. G. - Immune responses during human schistosomiasis mansoni. XI. Immunological status of patients with acute infections and after treatment. J. Immunol., 135: 2121-2127, 1985.
12. KAPLAN, M.E.; WOODSON, M. & CLARK. C. - Detection of human T lymphocytes by rosettte formation with AET treated sheep red cells. In: BLOOM, B. R. & DAVID. J. R., ed. - In vitro methods in cell mediated and tumor immunity. New York, Academic Press, 1976. p. 83-88.
13. KATZ, N.; CHAVES, A. & PELLEGRINO, J. - A simple device for quantitative stool thick smear technic in Schistosomiasis mansoni Rev. Inst. Med. trop. S. Paulo, 14: 397-400, 1972.
14. MACHADO, I.; RUIZ DIEZ, E.; BLANCA, I. & BIANCO N. - Characterization of cell mediated immunity in long term survivors of gastric or colorectal cancer. Amer J. Surg., 147: 334-338, 1984.
15. MORIEARTY. P. L. & LEWERT, R. M. - Delayed Hypersensivity in Uganda Schistosomiasis mansoni. III. Examination of serologic responses and clinical states. Amer. J. trop. Med. Hyg. 23: 190-196, 1974.
16. NOBUO, O.; MINAI, M. & TAKEIKO, S. - Antigen specific suppressor T lymphocytes (Leu 2_a + 3_a-) in human Schistosomiasis japonica. J. Immunol. 131: 2524-2527, 1984.
17. OLIVER GONZALEZ J. - Anti-egg precipitins in sera of human infected with Schistosoma mansoni. J. infect. Dis., 95: 86-91, 1954.
18. OTTESEN. E. A. - Modulation of the host response in human schistosomiasis. I. Adherent suppressor cells that inhibit lymphocyte proliferative responses to parasite an tigens. J. Immunol., 123: 1639-1644, 1979.
19. OTTESEN, E. A.; HIATT, R. A.; CHEEVES, A. W.; SOTOMAYOR, Z. R. & NEVA, F. A. - The acquisition and lost of antigens specific cellular immune responsiveness in acute and chronic schistosomiasis in man. Clin. exp. Immunol., 33: 38-47, 1978
20. ROCKLIN, R. E.; BROWN, A.; WARREN, K. S., PELLEG. R. P.; HOUBA, V.; SIONGOC, T. K.; OUMA, J.; STURROCK, R. & BUTERWOTH, A. E. - Factors that modify the cellular immune response in patients infected by Schistosoma mansoni J. Immunol., 125: 1916-1923, 1980
21. SHAH, S. A. & DICKSOW. J. A. - Lymphocyte separation from blood. Nature, 249: 168-169, 1974.
22. STOBO, J. D. - Immunossuppression in man: suppression by macrophages can be mediated by interactions with regulatory T lymphocytes. J. Immunol., 119: 918, 1977.
23. TIMONEN, T. & SAKSELA, E. - Isolation of human natural killer cells by density gradient centrifugation. J. Immunol. Meth., 36: 285, 1980.
24. TOOD, C. W.; GOODGAME, R. W. & COLLEY, D. G. - Immune responses during human Schistosomiasis mansoni. V. Suppression of schistosome antigen specific lymphocyte blastogenesis by adherent-phagocitic cells. J. Immunol., 122: 1440, 1979.
25. VOLLER, A.; BARTLETT, A. & BIDWELL, D. - Enzyme immunoassays for parasitic diseases. Trans. roy. Soc. trop. Med. Hyg., 70: 98-105, 1976.
26. WYSOCKI, L. J. & SATO. V. L. - "Panning" for lymphocytes a method for cell selection. Proc. nat. Acad. Sci. (Wash.)., 75: 2844, 1978.

Address for correspondence:

Oscar Aldrey, MD

Inmunología Clínica

Apartado 50647

Correos de Sabana Grande

Caracas 1051, Venezuela

(1

) Research supported by grants from CONICTT (Fortalecimiento de Centros) and from CDCH M 09.15 84 (UCV).

Publication Dates

Publication in this collection
17 Feb 2011
Date of issue
Dec 1988

History

Received
29 July 1987

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ALARCON DE NOYA, B.; NOYA, O.; BRITO DE URBAEZ, R. & RISQUEZ, J. - Reactivación de un foco de esquistosomiasis mansoni en Venezuela. Bol. Direcc. Malar., 27(1-4), 1987.

[2] 2. ARANGO, M.; OROPEZA, F.; ANDERSON, O.; CONTRERAS, C. E.; BIANCO, N. & YARZABAL, L. - Circulating immune complexes and the "in vitro" cell reactivity in paracoccidioidomycosis. Mycopathologia (Den Haag). 79: 153-158, 1982.

[3] 3. BARSOUM, I. S.; GAMIL, F.M.; & RAMZY, R. M. - Immune response and immunoregulation in relation to human schistosomiasis in Egypt. Effect of treatment on in vitro cellular responsiveness. Amer, J. trop. Med. Hyg. 31: 1181-1187, 1982.

[4] 4. BOYUM, A. - Isolation of mononuclear cells and granulocytes from human blood. Scand. J. Clin. Lab. Invest., 21 (supp. 97): 77, 1968.

[5] 5. CAPRON, M.; DESSAINT, J. P. & JOSEPH, M. - Effectors mechanisms against schistosoma. Progr. Immunol., 5: 1305-1316, 1983.

[6] 6. COLLEY, D. G : COOK, J. A. & JORDAN, P. - Immune responses during human schistosomiasis mansoni. I. In vitro lymphocyte blastogenic responses to heteregeneous antigenic preparations from schistosome eggs, worms and cercariae. Int. Arch. Allergy, 53: 420-433. 1979.

[7] 7. COLLEY, D. G., HIENY, S E.; BARTHOLOMEW, R. K. & COOK, J. A. - Immune responses during human Schistosomiasis mansoni. III. Regulatory effect of patient sera on human lymphocyte blastogenic responses to schistosome antigen preparations. Amer. J., trop. Med. Hyg., 26: 917-925, 1977.

[8] 8. COLLEY, D. G.; LEWIS, F. A. & GOODGAME, R. W. - Immune response during human Schistosomiasis mansoni. IV. Induction of suppressor cell activity by Schistosoma antigens preparations. J. Immunol., 120: 1225-1232, 1978.

[9] 9. CONTRERAS, C. E.; OROZCO, A.; SANCHEZ, P.; ORTEGA, G. & BIANCO. N. E. - Physiological aspect of circulating immune complexes in the normal population. Clin. exp. Immunol., 48. 693-699, 1982.

[10] 10. DAMIAN, R. - Immune schistosomiasis, a holistic view. Contemp. Top. Immunobiol., 12: 359-420, 1984.

[11] 11. GAZZINELLI, G.; LAMBERTUCCI, J. R.; KATZ, N.; ROCHA, R.; LIMA, M. & COLLEY, D. G. - Immune responses during human schistosomiasis mansoni. XI. Immunological status of patients with acute infections and after treatment. J. Immunol., 135: 2121-2127, 1985.

[12] 12. KAPLAN, M.E.; WOODSON, M. & CLARK. C. - Detection of human T lymphocytes by rosettte formation with AET treated sheep red cells. In: BLOOM, B. R. & DAVID. J. R., ed. - In vitro methods in cell mediated and tumor immunity. New York, Academic Press, 1976. p. 83-88.

[13] 13. KATZ, N.; CHAVES, A. & PELLEGRINO, J. - A simple device for quantitative stool thick smear technic in Schistosomiasis mansoni Rev. Inst. Med. trop. S. Paulo, 14: 397-400, 1972.

[14] 14. MACHADO, I.; RUIZ DIEZ, E.; BLANCA, I. & BIANCO N. - Characterization of cell mediated immunity in long term survivors of gastric or colorectal cancer. Amer J. Surg., 147: 334-338, 1984.

[15] 15. MORIEARTY. P. L. & LEWERT, R. M. - Delayed Hypersensivity in Uganda Schistosomiasis mansoni. III. Examination of serologic responses and clinical states. Amer. J. trop. Med. Hyg. 23: 190-196, 1974.

[16] 16. NOBUO, O.; MINAI, M. & TAKEIKO, S. - Antigen specific suppressor T lymphocytes (Leu 2_a + 3_a-) in human Schistosomiasis japonica. J. Immunol. 131: 2524-2527, 1984.

[17] 17. OLIVER GONZALEZ J. - Anti-egg precipitins in sera of human infected with Schistosoma mansoni. J. infect. Dis., 95: 86-91, 1954.

[18] 18. OTTESEN. E. A. - Modulation of the host response in human schistosomiasis. I. Adherent suppressor cells that inhibit lymphocyte proliferative responses to parasite an tigens. J. Immunol., 123: 1639-1644, 1979.

[19] 19. OTTESEN, E. A.; HIATT, R. A.; CHEEVES, A. W.; SOTOMAYOR, Z. R. & NEVA, F. A. - The acquisition and lost of antigens specific cellular immune responsiveness in acute and chronic schistosomiasis in man. Clin. exp. Immunol., 33: 38-47, 1978

[20] 20. ROCKLIN, R. E.; BROWN, A.; WARREN, K. S., PELLEG. R. P.; HOUBA, V.; SIONGOC, T. K.; OUMA, J.; STURROCK, R. & BUTERWOTH, A. E. - Factors that modify the cellular immune response in patients infected by Schistosoma mansoni J. Immunol., 125: 1916-1923, 1980

[21] 21. SHAH, S. A. & DICKSOW. J. A. - Lymphocyte separation from blood. Nature, 249: 168-169, 1974.

[22] 22. STOBO, J. D. - Immunossuppression in man: suppression by macrophages can be mediated by interactions with regulatory T lymphocytes. J. Immunol., 119: 918, 1977.

[23] 23. TIMONEN, T. & SAKSELA, E. - Isolation of human natural killer cells by density gradient centrifugation. J. Immunol. Meth., 36: 285, 1980.

[24] 24. TOOD, C. W.; GOODGAME, R. W. & COLLEY, D. G. - Immune responses during human Schistosomiasis mansoni. V. Suppression of schistosome antigen specific lymphocyte blastogenesis by adherent-phagocitic cells. J. Immunol., 122: 1440, 1979.

[25] 25. VOLLER, A.; BARTLETT, A. & BIDWELL, D. - Enzyme immunoassays for parasitic diseases. Trans. roy. Soc. trop. Med. Hyg., 70: 98-105, 1976.

[26] 26. WYSOCKI, L. J. & SATO. V. L. - "Panning" for lymphocytes a method for cell selection. Proc. nat. Acad. Sci. (Wash.)., 75: 2844, 1978.

Brasil

Brasil

Immunopathology of human schistosomiasis mansoni: I. Immunomodulatory influences on T cell function

Imunopatologia da esquistossomose mansônica humana: I. Influências imunorregulatórias sobre a função T

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History