Accessibility / Report Error

Glucogeno sintasa en helmintos parasitos: inhibicion por benzimidazoles

The inhibitor effect of Benzimidazoles in the glycogen synthetase of helminthics parasites

Resumos

Se ha determinado el effecto inhibidor sobre la actividad Glucogeno sintetasa (E.C.2.41.11) por parte de cuatro antihelminticos: Albendazol, Mebendazol, Parbendazol y Tiabendazol. Observandose que en todos los casos, es el Parbendazol quien ha demostrado un mayor poder inhibidor sobre la glucógeno sintetasa de Ascaris suum, Fasciola hepatica y Moniezia expansa. El Tiabendazol es el anti-helmintico que menor efecto inhibidor ha presentado sobre la enzima en los tres parasitos objeto de nuestro estudio. Con el presente trabajo y otros previstos en la misma linea, se pretende aportar nuevos datos acerca del aun desconocido locus de acción de estos antihelminticos.

Glucógeno sintetasa - inhibicion; Parbendazol; Mebendazol; Albendazol; Tiabendaúol; Ascaris suum; Fasciola hepatica; Moniezia expansa


The inhibitor effect on the Glycogen synthetase (E.C.2.4.1.11) activity by four anthelmintics: Mebendazole, Parbendazole, Albendazole and Thiabendazole was determinated. The most inhibitor effect of Parbendazole on A. suum, F. hepatica and M. expansa, was observed. The lowest inhibitor effect of Thiabendazole, on all cases, was also observed. The aim of our study, and further studies on the same line, are to provide new reports about the unknown locus of actuation of these anthelmintics.


Glucógeno sintetasa - inhibicion; Parbendazol; Mebendazol; Albendazol; Tiabendaúol; Ascaris suum; Fasciola hepatica; Moniezia expansa

ARTIGOS ORIGINAIS

Glucogeno sintasa en helmintos parasitos. inhibicion por benzimidazoles

The inhibitor effect of Benzimidazoles in the glycogen synthetase of helminthics parasites

H. Gomez-Banqueri; M. A. Garcia Ruiz; M. Monteoliva; M. Sanchez-Moreno

Sección de Bioquímica del Instituto "Lopez-Neyra" de Parasitologia C.S.I.C. C/ Ventanilla, 11. 18001-Granada (Spain)

Correspondência Correspondência: Dr. M. Sanchez-Moreno

RESUMEN

Se ha determinado el effecto inhibidor sobre la actividad Glucogeno sintetasa (E.C.2.41.11) por parte de cuatro antihelminticos: Albendazol, Mebendazol, Parbendazol y Tiabendazol. Observandose que en todos los casos, es el Parbendazol quien ha demostrado un mayor poder inhibidor sobre la glucógeno sintetasa de Ascaris suum, Fasciola hepatica y Moniezia expansa. El Tiabendazol es el anti-helmintico que menor efecto inhibidor ha presentado sobre la enzima en los tres parasitos objeto de nuestro estudio.

Con el presente trabajo y otros previstos en la misma linea, se pretende aportar nuevos datos acerca del aun desconocido locus de acción de estos antihelminticos.

Unitermos: Glucógeno sintetasa - inhibicion; Parbendazol; Mebendazol; Albendazol; Tiabendaúol; Ascaris suum; Fasciola hepatica; Moniezia expansa.

SUMMARY

The inhibitor effect on the Glycogen synthetase (E.C.2.4.1.11) activity by four anthelmintics: Mebendazole, Parbendazole, Albendazole and Thiabendazole was determinated. The most inhibitor effect of Parbendazole on A. suum, F. hepatica and M. expansa, was observed. The lowest inhibitor effect of Thiabendazole, on all cases, was also observed.

The aim of our study, and further studies on the same line, are to provide new reports about the unknown locus of actuation of these anthelmintics.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

Recebido para publicação em 20/8/85.

  • 1. CHAPPELL, L. H. - In: Physiology of Parasites. Glasgow, Blackie, 1980.
  • 2. CORNISH, R. A. & BRYANT, C. - Changes in energy metabolism due to anthelmintics in Fasciola hepatica maintained "in vitro". Int. J. Parasit., 6: 393-398, 1976.
  • 3. CZOK, R.; CZIFER, S. & JELINIC, B. - Glykogen metabolismes in Schistosoma mansoni. Wien. tierärztl. Mschr., 62: 249-254, 1975.
  • 4. DEDINGER, J. E. & ROBERTS, L. S. - Glycogen syntethase in the rat tapeworm, Hymenolepis diminuta. I. Enzyme activity during development and with crowding. Comp. Biochem. Physiol., 58B: 215-219, 1977.
  • 5. DONAHUE, M. S.; YACOUB, N. J.; KAEINI, M. R. & HARRIS, B. G. - Activity of enzyme regulating glycogen metabolism in perfused muscle-cuticle sections of Ascaris suum (Nematoda). J. Parasit., 67: 362-367, 1981.
  • 6. EVANS, W. S.; HARDY, M. & NOVAK, M. - A comparison of the effect of albendazole, cambendazole and thiabendazole on the larval development of three hy-menolepidid cestodes. J. Parasit., 66: 935-940, 1980.
  • 7. GOMEZ-BANQUERI, H.; SANCHEZ-MORENO, M.; TE-JADA, P. & MONTEOLIVA, M. - Aislamiento y purification de la Glucógeno Sintetasa en Ascaris suum (Nematode), Fasciola hepatica (Trematode) y Moniezia expansa. (En prensa, 1985).
  • 8. KELLY, J. D.; CHEVIS, R. A. F. & WHTTLOCK, H. V. - The anthelmintic efficacy of mebendazole against adult Fasciola hepatica and a concurrent mixed nematode infection in sheep. N. Z. vet. J., 23: 81-84, 1975.
  • 9. KLEIN, J.; ZKHARENKO, D. F.; DOLGINA, L. E.; BRAGINETZ, W. R. & LINKO, I. A. In: KIM, C. W.; RUITEMBERG, E. J. & TEPPEMA, J. S. - Trichinellosis. Surrey, Reedbooks, 1981. p. 291-296.
  • 10. KOMUNIECKI, P. R. & SAZ, H. J. - The effect of levamisole on Glycogen synthase and the metabolism of Litosomoides carinii. J. Parasit. 68: 221-227, 1982.
  • 11. LELOIR, L. F. & GOLDEMBERG, S. A. - Glycogen synthase from rat liver. Meth. Enzymol., 5: 145, 1962.
  • 12. LOWRY, O. H.; ROSEBROUGH, N. J.; FARR, A. L. & RANDALL, R. J. - Protein measurement with the Folin phenol reagent. J. biol. Chem., 193: 265-275, 1951.
  • 13. MIED, P. A. & BUEDING, E. - Glycogen synthase of Hymenolepis diminuta. I. Allosteric activation and inhibition. J. Parasit., 65: 14-24, 1979.
  • 14. PRICHARD, R. K. - Mode of action of the anthelmintic Thiabendazole in Haemonchus contortus. Nature, 228: 684-685, 1970.
  • 15. PRICHARD, R. K. - The fumarate reductase reaction of Haemonchus contortus and the mode of action of some anthelmintics. Int. J. Parasit., 3: 409-417, 1973.
  • 16. ROTMANS, J. P. - Glycogen synthase of Schistosoma mansoni: a preliminary report. Acta leidensía, 48: 29-36, 1980.
  • 17. VAN DEN BQSSCHE, H. & DE NOLLIN, S. - Effects of mebendazole on the absortion of low molecular weight nutrients by Ascaris suum. Int. J. Parasit., 3: 401-407, 1973.
  • Correspondência:
    Dr. M. Sanchez-Moreno
  • Datas de Publicação

    • Publicação nesta coleção
      17 Fev 2011
    • Data do Fascículo
      Ago 1987

    Histórico

    • Recebido
      20 Ago 1985
    Instituto de Medicina Tropical de São Paulo Av. Dr. Enéas de Carvalho Aguiar, 470, 05403-000 - São Paulo - SP - Brazil, Tel. +55 11 3061-7005 - São Paulo - SP - Brazil
    E-mail: revimtsp@usp.br