Human papillomavirus detection in cervical dysplasias or neoplasias and in condylomata acuminaata by in situ hybridization with biotinylated DNA probes

Detecção de papilomavirus humano em displasias ou neoplasias cervicais e em condilomas acuminados por hibridização in situ com sondas de DNA biotiniladas

Abstracts

Specimens from cervical dysplasias or carcinomas and genital condylomata acuminata were retrospectively analysed by in situ hybridization (ISH) with bioti-nylated DNA probes for human papillomavirus (HPV) types 6, 11, 16 and 18. In the control group no case was positive for HPV DNA. In mild/moderate dysplasias, 4 cases (14%) were positive for HPV 6 or 11 and 2 cases (7%), for HPV 16. In the severe dysplasia/in situ carcinoma group, 9 cases (31%) showed presence of DNA of HPV types 16 or 18. Six invasive carcinomas (20%) were positive for HPV type 16 or 18. Among condylomata acuminata, 22 cases (73%) were positive for HPV types 6 or 11. In all ISH-positive cases only one viral type was detected. No correlation between HPV DNA positivity and histological findings of HPV infection was observed. Although less sensitive than some other molecular biology techniques, in situ hybridization with biotinylated DNA probes proved to be simple and useful for detecting and typing HPV in samples routinely received for histopathological analysis.

Human papillomavirus; In situ hybridization; Cervical dysplasia; Cervical cancer; Condylomata acuminata


Amostras de displasias ou carcinomas do colo uterino e de condilomas acuminados da região genital foram analisados retrospectivamente por hibridização in situ (HIS) com sondas de DNA biotiniladas de papilomavirus humano (HPV) tipos 6, 11, 16 e 18. Nenhum caso do grupo controle foi positivo para DNA de HPV. Em displasias leve/moderada, 4 casos (14%) foram positivos para HPV 6 ou 11 e 2 casos (7%), para HPV 16. No grupo de displasia acentuada/carcinoma in situ, 9 casos (31%) tinham DNA de HPV tipos 16 ou 18. Seis carcinomas invasores (20%) foram positivos para HPV tipos 16 ou 18. Entre os condilomas acuminados, 22 casos (73%) foram positivos para HPV tipos 6 ou 11. Em todos os casos positivos pela HIS somente um tipo viral foi encontrado. Não foi observada correlação entre a positividade para DNA de HPV e achados histológicos de infecção por HPV. Apesar de menos sensível que algumas outras técnicas de biologia molecular, a hibridização in situ com sondas de DNA biotiniladas mostrou-se simples e adequada para detecção e tipagem de HPV em amostras enviadas rotineiramente para estudo histopatológico.


ORIGINAL ARTICLES

Human papillomavirus detection in cervical dysplasias or neoplasias and in condylomata acuminaata by in situ hybridization with biotinylated DNA probes

Detecção de papilomavirus humano em displasias ou neoplasias cervicais e em condilomas acuminados por hibridização in situ com sondas de DNA biotiniladas

Eliane Machado GuimarãesI; Geraldo Brasileiro FilhoI; Sérgio Danilo Junho PenaII

IDepartamento de Anatomia Patológica e Medicina Legal, Faculdade de Medicina, UFMG. Belo Horizonte, MG, Brasil

IIDepartamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, UFMG. Belo Horizonte, MG, Brasil

Address for correspondence

SUMMARY

Specimens from cervical dysplasias or carcinomas and genital condylomata acuminata were retrospectively analysed by in situ hybridization (ISH) with bioti-nylated DNA probes for human papillomavirus (HPV) types 6, 11, 16 and 18. In the control group no case was positive for HPV DNA. In mild/moderate dysplasias, 4 cases (14%) were positive for HPV 6 or 11 and 2 cases (7%), for HPV 16. In the severe dysplasia/in situ carcinoma group, 9 cases (31%) showed presence of DNA of HPV types 16 or 18. Six invasive carcinomas (20%) were positive for HPV type 16 or 18. Among condylomata acuminata, 22 cases (73%) were positive for HPV types 6 or 11.

In all ISH-positive cases only one viral type was detected. No correlation between HPV DNA positivity and histological findings of HPV infection was observed.

Although less sensitive than some other molecular biology techniques, in situ hybridization with biotinylated DNA probes proved to be simple and useful for detecting and typing HPV in samples routinely received for histopathological analysis.

Key words: Human papillomavirus; In situ hybridization; Cervical dysplasia; Cervical cancer; Condylomata acuminata.

RESUMO

Amostras de displasias ou carcinomas do colo uterino e de condilomas acuminados da região genital foram analisados retrospectivamente por hibridização in situ (HIS) com sondas de DNA biotiniladas de papilomavirus humano (HPV) tipos 6, 11, 16 e 18. Nenhum caso do grupo controle foi positivo para DNA de HPV. Em displasias leve/moderada, 4 casos (14%) foram positivos para HPV 6 ou 11 e 2 casos (7%), para HPV 16. No grupo de displasia acentuada/carcinoma in situ, 9 casos (31%) tinham DNA de HPV tipos 16 ou 18. Seis carcinomas invasores (20%) foram positivos para HPV tipos 16 ou 18. Entre os condilomas acuminados, 22 casos (73%) foram positivos para HPV tipos 6 ou 11.

Em todos os casos positivos pela HIS somente um tipo viral foi encontrado. Não foi observada correlação entre a positividade para DNA de HPV e achados histológicos de infecção por HPV.

Apesar de menos sensível que algumas outras técnicas de biologia molecular, a hibridização in situ com sondas de DNA biotiniladas mostrou-se simples e adequada para detecção e tipagem de HPV em amostras enviadas rotineiramente para estudo histopatológico.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

Recebido para publicação em 03/12/1991

Aceito para publicação em 13/02/1992

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  • Address for correspondence:
    Prof. Geraldo Brasileiro Filho
    Departamento de Anatomia Patológica
    Faculdade de Medicina UFMG
    Caixa Postal, 340
    30.130, Belo Horizonte-MG, Brasil

Publication Dates

  • Publication in this collection
    12 Sept 2006
  • Date of issue
    Aug 1992

History

  • Received
    03 Dec 1991
  • Accepted
    13 Feb 1992
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