Parasitological and serological studies on amoebiasis and other intestinal parasitic infections in the rural sector around Recife, Northeast Brazil

Estudos sorológicos e parasitológicos na amebíase e em outras infecções parasito-intestinais no setor rural dos arredores de Recife, nordeste do Brasil

José Felipe Gonçalves Masanobu Tanabe Francisco de Paula de Melo Medeiros Fernando José Gonçalves Ivanize da Silva Aca Severa Regina Nunes da Motta Seiki Tateno Tsutomu Takeuchi About the authors

Abstracts

Parasitological examinations were carried out during July to December, 1989, on 485 inhabitants of four villages in São Lourenço da Mata, 25 km northwest of Recife, Pernambuco, Brazil. Approximately 99.6% of the inhabitants were infected with at least one species of intestinal parasites. A high prevalence of Schistosoma mansoni (82.1%), hookworm (80.2%) Trichuris trichiura (69.9%), Ascaris lumbricoides (61.9%) and Entamoeba coli (36.7%) infections were demonstrated. Test tube cultivation revealed that the most common species of hookworm in this region was Necator americanus (88.4%), and also that the prevalence of Strongyloides stercoralis was 5.8%. Three hundred and thirty-four sera were serologically examined for amoebiasis by the gel diffusion precipitation test (GDP) and enzyme-linked immunosorbent assay (ELISA). No positive reaction was observed in all sera as examined by GDP, while 24 sera were positive by ELISA.

Parasitic helminths; Parasitic protozoa; Amoebiasis; Entamoeba histolytica; Schistosoma mansoni; Stool examination; Sero-epidemiology; Northeast Brazil


Foram realizados exames parasitológicos em 485 habitantes de quatro vilarejos da cidade de São Lourenço da Mata, distante 25 km à noroeste de Recife-PE, Brasil, no período de julho à dezembro de 1989. Aproximadamente 99,6% dos examinados mostraram-se infectados com pelo menos uma espécie de parasita intestinal. Observou-se ainda uma alta prevalência de Schistosoma mansoni (82,1%), ancilostomídeos (80,2%), Trichuris trichiura (69,9%), Ascaris lumbricoides (61,9%) e Entamoeba coli (36,7%). A cultura de larvas de ancilostomídeos nas fezes - método de Harada - revelou que Necator americanus (84,4%) é a espécie mais comum nesta região seguida pelo Strongyloides stercoralis (5,8%). Foram ainda realizados testes sorológicos - imunodifusão em gel (GDP) e enzima imunoensaio (ELISA) - em 334 soros, para o diagnóstico de amebíase, todavia apenas 24 (7,2%) dos soros apresentaram positividade no teste de ELISA e nenhuma positividade foi encontrada nos mesmos soros testados pelo GDP.


EPIDEMIOLOGY

Parasitological and serological studies on amoebiasis and other intestinal parasitic infections in the rural sector around Recife, Northeast Brazil

Estudos sorológicos e parasitológicos na amebíase e em outras infecções parasito-intestinais no setor rural dos arredores de Recife, nordeste do Brasil

José Felipe GonçalvesI; Masanobu TanabeII,III; Francisco de Paula de Melo MedeirosIII; Fernando José GonçalvesI; Ivanize da Silva AcaII,III,IV; Severa Regina Nunes da MottaII,III,V; Seiki TatenoII,III; Tsutomu TakeuchiII

ICentro de Pesquisas Aggeu Magalhães, Recife, Pernambuco, Brasil

IIDepartment of Parasitology, School of Medicine, Keio University, Shinjuku, Tokyo, Japan

IIILaboratório de Imunopatologia Prof. Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE). Recife, Pernambuco, Brasil

IVDepartamento de Medicina Tropical, UFPE. Recife, Pernambuco, Brasil

VDepartamento de Biofísica e Radiobiología, UFPE. Recife, Pernambuco, Brasil

Address for correspondence

SUMMARY

Parasitological examinations were carried out during July to December, 1989, on 485 inhabitants of four villages in São Lourenço da Mata, 25 km northwest of Recife, Pernambuco, Brazil. Approximately 99.6% of the inhabitants were infected with at least one species of intestinal parasites. A high prevalence of Schistosoma mansoni (82.1%), hookworm (80.2%) Trichuris trichiura (69.9%), Ascaris lumbricoides (61.9%) and Entamoeba coli (36.7%) infections were demonstrated. Test tube cultivation revealed that the most common species of hookworm in this region was Necator americanus (88.4%), and also that the prevalence of Strongyloides stercoralis was 5.8%.

Three hundred and thirty-four sera were serologically examined for amoebiasis by the gel diffusion precipitation test (GDP) and enzyme-linked immunosorbent assay (ELISA). No positive reaction was observed in all sera as examined by GDP, while 24 sera were positive by ELISA.

Key words: Parasitic helminths; Parasitic protozoa; Amoebiasis; Entamoeba histolytica; Schistosoma mansoni; Stool examination; Sero-epidemiology; Northeast Brazil.

RESUMO

Foram realizados exames parasitológicos em 485 habitantes de quatro vilarejos da cidade de São Lourenço da Mata, distante 25 km à noroeste de Recife-PE, Brasil, no período de julho à dezembro de 1989. Aproximadamente 99,6% dos examinados mostraram-se infectados com pelo menos uma espécie de parasita intestinal. Observou-se ainda uma alta prevalência de Schistosoma mansoni (82,1%), ancilostomídeos (80,2%), Trichuris trichiura (69,9%), Ascaris lumbricoides (61,9%) e Entamoeba coli (36,7%).

A cultura de larvas de ancilostomídeos nas fezes — método de Harada — revelou que Necator americanus (84,4%) é a espécie mais comum nesta região seguida pelo Strongyloides stercoralis (5,8%).

Foram ainda realizados testes sorológicos — imunodifusão em gel (GDP) e enzima imunoensaio (ELISA) — em 334 soros, para o diagnóstico de amebíase, todavia apenas 24 (7,2%) dos soros apresentaram positividade no teste de ELISA e nenhuma positividade foi encontrada nos mesmos soros testados pelo GDP.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

ACKNOWLEDGEMENT

This study was supported in part by research grants from Japan International Cooperation Agency (JICA), Ooyama Health Foundation, Keio University and the Ministry of Education, Science and Culture (No. 01570221), Tokyo, Japan.

  • Address for correspondence:
    Masanobu Tanabe, Ph. D.
    Department of Parasitology, School of Medicine Keio University
    Shinano-machi 35
    Shinjuku-ku, Tokyo 160, Japan
  • Recebido para publicação em 20/3/1990.

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    Address for correspondence: Masanobu Tanabe, Ph. D. Department of Parasitology, School of Medicine Keio University Shinano-machi 35 Shinjuku-ku, Tokyo 160, Japan

    Publication Dates

    • Publication in this collection
      11 Sept 2006
    • Date of issue
      Dec 1990

    History

    • Received
      20 Mar 1990
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