Evaluation of phenotypic markers associated with pathogenicity in the genus Listeria

Nunes, Zilma das Graças; Hofer, Ernesto

doi:10.1590/S0036-46651994000400001

Abstracts

A total of 130 Listeria strains were tested in order to evaluate lecithinase production and capacity for Congo red adsorption as markers of pathogenicity. The strains were identified according to acid production from sugars and by the CAMP test and the data were correlated with the ability to produce keratoconjunctivitis in guinea pigs. L. monocytogenes cultures presented 51.8% and 88.8% positivity rates for Congo red adsorption and lecithinase production, respectively, whereas 80.8% and 100% for L. innocua cultures were negative for the two test, respectively

Listeria monocytogenes; Hemolysin; Lecithinase; Congo red adsorption; Keratoconjunctivitis

Com propósito de avaliar a produção de lecitinase e a capacidade de adsorção do corante vermelho Congo como marcadores de patogenicidade, foram estudadas 130 amostras de Listeria. Estas amostras foram identificadas segundo a produção de ácido a partir de açúcares aliada ao teste CAMP, correlacionando-se estes dados à capacidade de produção de ceratoconjuntivite em cobaio. As culturas de L. monocytogenes apresentaram taxas de positividade para a adsorção do corante e produção de lecitinase de 51,8 e 88,8%, respectivamente, enquanto 80,8% e 100% das culturas de L. innocua foram negativas para os referidos testes.

MICROBIOLOGY

Evaluation of phenotypic markers associated with pathogenicity in the genus Listeria

Avaliação de marcadores fenotípicos associados à patogenicidade no gênero Listeria

Zilma das Graças Nunes; Ernesto Hofer^I

^IDepartment of Bacteriology, Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil, 4365; Manguinhos; 21045-900 Rio de Janeiro, RJ, Brasil

SUMMARY

A total of 130 Listeria strains were tested in order to evaluate lecithinase production and capacity for Congo red adsorption as markers of pathogenicity. The strains were identified according to acid production from sugars and by the CAMP test and the data were correlated with the ability to produce keratoconjunctivitis in guinea pigs.

L. monocytogenes cultures presented 51.8% and 88.8% positivity rates for Congo red adsorption and lecithinase production, respectively, whereas 80.8% and 100% for L. innocua cultures were negative for the two test, respectively.

Keywords:Listeria monocytogenes; Hemolysin; Lecithinase; Congo red adsorption; Keratoconjunctivitis.

RESUMO

Com propósito de avaliar a produção de lecitinase e a capacidade de adsorção do corante vermelho Congo como marcadores de patogenicidade, foram estudadas 130 amostras de Listeria. Estas amostras foram identificadas segundo a produção de ácido a partir de açúcares aliada ao teste CAMP, correlacionando-se estes dados à capacidade de produção de ceratoconjuntivite em cobaio.

As culturas de L. monocytogenes apresentaram taxas de positividade para a adsorção do corante e produção de lecitinase de 51,8 e 88,8%, respectivamente, enquanto 80,8% e 100% das culturas de L. innocua foram negativas para os referidos testes.

Full text available only in PDF format.

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ACKNOWLEDGMENT

We thank Rosmery Ribeiro for the technical assistance.

Recebido para publicação em 08/10/1993

Aceito para publicação em 17/05/1994

1. FARBER, J.M. & SPEIRS, J.I. - Potential use continuous cell lines to distinguish between pathogenic and nonpathogenic Listeria spp. J. clin. Microbiol., 25: 1463-1466, 1987.
2. FRASER, G. - A plate method for the rapid identification of Listeria (Erysipelothrix) monocytogenes. Vet. Rec., 74: 50-51, 1962.
3. FÜZI, M. & PILLIS, I. - Production of opacity in egg-yolk medium by Listeria monocytogenes. Nature, 196: 195, 1962.
4. GAILLARD, J.L.; BERCHE, P. & SANSONETTI, P. - Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes. Infect. Immun., 52: 50-55, 1986.
5. GEOFFROY, C.; GAILLARD, J.L.; ALOUF, J.E. & BERCHE, P. - Purification, characterization and toxicity of the sulphydryl - activated hemolysin listeriolysin O from Listeria monocytogenes. Infect. Immun., 55: 1641-1646, 1987.
6. GROVES, R.D. & WELSHIMER, H.J. - Separation of pathogenic from apathogenic Listeria monocytogenes by three in vitro reactions. J. clin. Microbiol., 5: 559-563, 1977.
7. HOFER, E. - Contribuiçăo ao estudo epidemiológico da ocorręncia de portadores de Listeria monocytogenes entre operários de matadouro e indivíduos com distúrbios entéricos. Rio de Janeiro, 1974. (Tese de Livre Docencia - Instituto de Veterinária da Universidade Federal Rural do Rio de Janeiro).
8. HOFER, E. - Bacteriologic and epidemiologic studies on the occurrence of Listeria monocytogenes in healthy cattle. Zbl. Bakt. Microbiol. Hyg. Abt. I Orig. A, 256: 175-183, 1983.
9. JENKINS, E.M.; NJOKU-OBI, A.N. & ADAMS, E.W. - Purification of the soluble hemolysins of Listeria monocytogenes. J. Bact., 88: 418-424, 1964.
10. JENKINS, E.M.; ADAMS, E.W. & WATSON, B.B. - Further investigations on the production and nature of the soluble hemolysins of Listeria monocytogenes. In: INTERNATIONAL SYMPOSIUM OF LISTERIOSIS, 3, Bilthoven, Volksgezondheid, Utrech, Rijks Inst., 1966. Proceedings p. 109-123.
11. KATHARIOU, S.; ROCOURT, J.; HOF, H. & GOEBEL, W. - Levels of Listeria monocytogenes hemolysin are not directly proportional to virulence in experimental infection of mice. Infect. Immun., 56: 534-536, 1988.
12. KHAN, M.A.; SEAMAN, A. & WOODBINE, M. - Listeria monocytogenes - hemolysin: lecithinase. Zbl Bakt., I Abt. I, 225: 66-79, 1973.
13. LEIMESTER-WATCHER, M.; CHAKRABORTY, T. & GOEBEL, W. - Detection and presence of two hemolytic factors in Listeria spp. Ann. Inst. Pasteur Microbiol. (Paris), 138: 149-286, 1987.
14. MAURELLI, A.T.; BLACKMON, B. & CURTIS III, R. - Loss of pigmentation in Shigella flexneri 2a is correlated with loss of virulence and virulence-associated plasmid. Infect. Immun., 43: 397-401, 1984.
15. McLAUCHLIN, J. - Listeria monocytogenes, recent advances in the taxonomy and epidemiology of listeriosis in human. J. appl. Bact., 63: 1-11, 1987.
16. RALOVICH, B.S.; SHAHAMAT, M. & WOODBINE, M. - Further data on the characters of Listeria strains. Med. Microbiol. Immunol., 163: 125-139, 1977.
17. ROCOURT, J.; SCHRETTENBRUNNER, A. & SEELIGER, H.P.R. - Differenciation bioquimique des groups génomiques de Listeria monocytogenes (sensu lato). Ann. Inst. Pasteur Microbiol. (Paris), 134A: 65-71, 1983.
18. RODRIGUEZ, J.A.; BOLAND, J.A.V.; GARAYZABAL, J.F.F. et al. - Microplate technique to determine hemolytic activity for routine typing of Listeria strains. J. clin. Microbiol., 24: 99-103, 1986.
19. SASAKAWA, C.; KAMATA, K.; SAKAY, T. et al. - Molecular alteration of the 140 - megadalton plasmid associated with loss of virulence and Congo red binding activity in Shigella flexneri. Infect. Immunol., 51: 470-475, 1986.
20. SCHLECH, W.F. - Virulence characteristics of Listeria monocytogenes. Food Tech., 42: 176-178, 1988.
21. SEELIGER, H.P.R. - Apathogene listerien: L. innocua sp. n. (Seelinger et Schoofs, 1977). Zbl Bakt. Microbiol. Hyg. Abt. I Orig. A, 249: 487-493, 1981.
22. SKALKA, B.; SMOLA, J. & ELISCHEROVA, K. - Routine test for in vitro differentiation of pathogenic and apathogenic Listeria monocytogenes strains. J. clin. Microbiol., 15: 503-507, 1982.
23. SKALKA, B.; SMOLA, J. & ELISCHEROVA, K. - Hemolytic phenomenon under the cultivation of Listeria innocua. Zbl. Bakt. Microbiol. Hyg. Abt. I Orig. A., 253: 559-565, 1982.

Publication Dates

Publication in this collection
20 Sept 2006
Date of issue
Aug 1994

History

Accepted
17 May 1994
Received
08 Oct 1993

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. FARBER, J.M. & SPEIRS, J.I. - Potential use continuous cell lines to distinguish between pathogenic and nonpathogenic Listeria spp. J. clin. Microbiol., 25: 1463-1466, 1987.

[2] 2. FRASER, G. - A plate method for the rapid identification of Listeria (Erysipelothrix) monocytogenes. Vet. Rec., 74: 50-51, 1962.

[3] 3. FÜZI, M. & PILLIS, I. - Production of opacity in egg-yolk medium by Listeria monocytogenes. Nature, 196: 195, 1962.

[4] 4. GAILLARD, J.L.; BERCHE, P. & SANSONETTI, P. - Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes. Infect. Immun., 52: 50-55, 1986.

[5] 5. GEOFFROY, C.; GAILLARD, J.L.; ALOUF, J.E. & BERCHE, P. - Purification, characterization and toxicity of the sulphydryl - activated hemolysin listeriolysin O from Listeria monocytogenes. Infect. Immun., 55: 1641-1646, 1987.

[6] 6. GROVES, R.D. & WELSHIMER, H.J. - Separation of pathogenic from apathogenic Listeria monocytogenes by three in vitro reactions. J. clin. Microbiol., 5: 559-563, 1977.

[7] 7. HOFER, E. - Contribuiçăo ao estudo epidemiológico da ocorręncia de portadores de Listeria monocytogenes entre operários de matadouro e indivíduos com distúrbios entéricos. Rio de Janeiro, 1974. (Tese de Livre Docencia - Instituto de Veterinária da Universidade Federal Rural do Rio de Janeiro).

[8] 8. HOFER, E. - Bacteriologic and epidemiologic studies on the occurrence of Listeria monocytogenes in healthy cattle. Zbl. Bakt. Microbiol. Hyg. Abt. I Orig. A, 256: 175-183, 1983.

[9] 9. JENKINS, E.M.; NJOKU-OBI, A.N. & ADAMS, E.W. - Purification of the soluble hemolysins of Listeria monocytogenes. J. Bact., 88: 418-424, 1964.

[10] 10. JENKINS, E.M.; ADAMS, E.W. & WATSON, B.B. - Further investigations on the production and nature of the soluble hemolysins of Listeria monocytogenes. In: INTERNATIONAL SYMPOSIUM OF LISTERIOSIS, 3, Bilthoven, Volksgezondheid, Utrech, Rijks Inst., 1966. Proceedings p. 109-123.

[11] 11. KATHARIOU, S.; ROCOURT, J.; HOF, H. & GOEBEL, W. - Levels of Listeria monocytogenes hemolysin are not directly proportional to virulence in experimental infection of mice. Infect. Immun., 56: 534-536, 1988.

[12] 12. KHAN, M.A.; SEAMAN, A. & WOODBINE, M. - Listeria monocytogenes - hemolysin: lecithinase. Zbl Bakt., I Abt. I, 225: 66-79, 1973.

[13] 13. LEIMESTER-WATCHER, M.; CHAKRABORTY, T. & GOEBEL, W. - Detection and presence of two hemolytic factors in Listeria spp. Ann. Inst. Pasteur Microbiol. (Paris), 138: 149-286, 1987.

[14] 14. MAURELLI, A.T.; BLACKMON, B. & CURTIS III, R. - Loss of pigmentation in Shigella flexneri 2a is correlated with loss of virulence and virulence-associated plasmid. Infect. Immun., 43: 397-401, 1984.

[15] 15. McLAUCHLIN, J. - Listeria monocytogenes, recent advances in the taxonomy and epidemiology of listeriosis in human. J. appl. Bact., 63: 1-11, 1987.

[16] 16. RALOVICH, B.S.; SHAHAMAT, M. & WOODBINE, M. - Further data on the characters of Listeria strains. Med. Microbiol. Immunol., 163: 125-139, 1977.

[17] 17. ROCOURT, J.; SCHRETTENBRUNNER, A. & SEELIGER, H.P.R. - Differenciation bioquimique des groups génomiques de Listeria monocytogenes (sensu lato). Ann. Inst. Pasteur Microbiol. (Paris), 134A: 65-71, 1983.

[18] 18. RODRIGUEZ, J.A.; BOLAND, J.A.V.; GARAYZABAL, J.F.F. et al. - Microplate technique to determine hemolytic activity for routine typing of Listeria strains. J. clin. Microbiol., 24: 99-103, 1986.

[19] 19. SASAKAWA, C.; KAMATA, K.; SAKAY, T. et al. - Molecular alteration of the 140 - megadalton plasmid associated with loss of virulence and Congo red binding activity in Shigella flexneri. Infect. Immunol., 51: 470-475, 1986.

[20] 20. SCHLECH, W.F. - Virulence characteristics of Listeria monocytogenes. Food Tech., 42: 176-178, 1988.

[21] 21. SEELIGER, H.P.R. - Apathogene listerien: L. innocua sp. n. (Seelinger et Schoofs, 1977). Zbl Bakt. Microbiol. Hyg. Abt. I Orig. A, 249: 487-493, 1981.

[22] 22. SKALKA, B.; SMOLA, J. & ELISCHEROVA, K. - Routine test for in vitro differentiation of pathogenic and apathogenic Listeria monocytogenes strains. J. clin. Microbiol., 15: 503-507, 1982.

[23] 23. SKALKA, B.; SMOLA, J. & ELISCHEROVA, K. - Hemolytic phenomenon under the cultivation of Listeria innocua. Zbl. Bakt. Microbiol. Hyg. Abt. I Orig. A., 253: 559-565, 1982.

Brasil

Brasil

Evaluation of phenotypic markers associated with pathogenicity in the genus Listeria

Avaliação de marcadores fenotípicos associados à patogenicidade no gênero Listeria

Abstracts

Publication Dates

History