Prevalence of anti-p: Falciparum sporozoite antibodies in adults in the amapa region of Brazil

Prevalência de anticorpos anti-esporozoitos de P: Falciparum em soros da região do Amapá, Brasil

Virgílio Do Rosario Michael R. Hollingdale About the authors

Abstracts

17 of 20 adult sera from the Amapa region of Brazil were active in the inhibition of P. falciparum sporozoite invasion (ISI) assay which has been correlated with protective antibodies. In contrast 11 sera were positive in IFA tests and 6 were positive in CSP tests. These results suggest that the ISI assay will be useful for evaluating naturally acquired protective anti-sporozoite antibodies in endemic areas, particularly during vaccine efficacy studies using sporozoite-based vaccines.

Anti-sporozoite antibody; inhibiton of sporozoite invasion (ISI) assay; circumsporozoite precipitation (CSP) assay; immunofluorescent antibody (IFA) assay


17 de 20 soros humanos coletados na região de Amapá (Brasil) mostraram atividade nos testes de inibição de invasão de esporozoitos (ISI) in vitro que estão correlacionados com proteção por presença de anticorpos. Em contraste 11 soros foram positivos para IFA e 6 foram positivos para CSP. Estes resultados sugerem que ISI seja um ensaio mais adequado para medir respostas imunológicas em futuros estudos sobre eficácia de uma potencial vacina anti-malárica.


EPIDEMIOLOGIA

Prevalence of anti-p. Falciparum sporozoite antibodies in adults in the amapa region of Brazil

Prevalência de anticorpos anti-esporozoitos de P. Falciparum em soros da região do Amapá, Brasil

Virgílio Do Rosario; Michael R. Hollingdale

Biomedical Research Institute, 1211 Parklawn Drive Rockville, Maryland 20852 USA

SUMMARY

17 of 20 adult sera from the Amapa region of Brazil were active in the inhibition of P. falciparum sporozoite invasion (ISI) assay which has been correlated with protective antibodies. In contrast 11 sera were positive in IFA tests and 6 were positive in CSP tests. These results suggest that the ISI assay will be useful for evaluating naturally acquired protective anti-sporozoite antibodies in endemic areas, particularly during vaccine efficacy studies using sporozoite-based vaccines.

Key-words: Anti-sporozoite antibody; inhibiton of sporozoite invasion (ISI) assay; circumsporozoite precipitation (CSP) assay; immunofluorescent antibody (IFA) assay.

RESUMO

17 de 20 soros humanos coletados na região de Amapá (Brasil) mostraram atividade nos testes de inibição de invasão de esporozoitos (ISI) in vitro que estão correlacionados com proteção por presença de anticorpos. Em contraste 11 soros foram positivos para IFA e 6 foram positivos para CSP. Estes resultados sugerem que ISI seja um ensaio mais adequado para medir respostas imunológicas em futuros estudos sobre eficácia de uma potencial vacina anti-malárica.

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ACKNOWLEDGEMENTS

The support and assistance of Dr. F. J. Lopez Antunano, Pan American Health Organization is gratefully appreciated. This study was supported in part by the United States Agency for International Development, Contract No DPE-0453-C-00-3051.

We thank the staff of the Institute Evandro Chagas (P. SESP), Malaria Research Unit for providing us with the sera samples.

Recebido para publicação em 18/06/86.

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Publication Dates

  • Publication in this collection
    17 Feb 2011
  • Date of issue
    Feb 1987

History

  • Received
    18 June 1986
Instituto de Medicina Tropical de São Paulo Av. Dr. Enéas de Carvalho Aguiar, 470, 05403-000 - São Paulo - SP - Brazil, Tel. +55 11 3061-7005 - São Paulo - SP - Brazil
E-mail: revimtsp@usp.br
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