Differences in the antigenic profile of bloodstream and cell culture derived trypomastigotes of Trypanosoma cruzi

Diferenças no perfil antigênico de tripomastigotas sangüícolas e de cultura celular do Trypanosoma cruzi

Abstracts

A comparative study of the antigenic profile of bloodstream and cell culture derived trypomastigotes showed many differences in their components. Using mouse anti-T. cruzi antibodies the differences were located mostly in the 120 kDa band, whereas using chagasic patient sera the differences were located in the 85 and 52 kDa bands. These findings might explain known physiological differences between trypomatigotes obtained from cell culture and from infected blood. A brief report of this work has already been published9.

Trypanosoma cruzi; T. cruzi antigens; Trypomastigotes


O estudo comparativo do perfil antigênico de tripomastigotas sangüícolas e tripomatigotas obtidos por cultura celular do Trypanosoma cruzi revelou que estas formas apresentam diferenças em alguns de seus componentes. Utilizando-se anticorpos provenientes da infecção murina, as diferenças foram detectadas na região de 120 kDa enquanto soros de pacientes chagásicos detectaram diferenças nas regiões de 85 e 52 kDa. Estas observações podem oferecer uma explicação a diferenças fisiológicas que ocorrem entre os tripomastigotas sangüícolas e os obtidos de cultura celular.


ORIGINAL ARTICLES

Differences in the antigenic profile of bloodstream and cell culture derived trypomastigotes of Trypanosoma cruzi

Diferenças no perfil antigênico de tripomastigotas sangüícolas e de cultura celular do Trypanosoma cruzi

Ana M. M. Da Silva; Cláudia I. Brodskyn; Harumi A. Takehara; I. Mota

Centro de Pesquisa e Formação em Imunologia da OMS/OPS. Instituto Butantan, São Paulo, Brasil

Address for correspondence

SUMMARY

A comparative study of the antigenic profile of bloodstream and cell culture derived trypomastigotes showed many differences in their components. Using mouse anti-T. cruzi antibodies the differences were located mostly in the 120 kDa band, whereas using chagasic patient sera the differences were located in the 85 and 52 kDa bands. These findings might explain known physiological differences between trypomatigotes obtained from cell culture and from infected blood. A brief report of this work has already been published9.

Key words:Trypanosoma cruzi; T. cruzi antigens; Trypomastigotes.

RESUMO

O estudo comparativo do perfil antigênico de tripomastigotas sangüícolas e tripomatigotas obtidos por cultura celular do Trypanosoma cruzi revelou que estas formas apresentam diferenças em alguns de seus componentes. Utilizando-se anticorpos provenientes da infecção murina, as diferenças foram detectadas na região de 120 kDa enquanto soros de pacientes chagásicos detectaram diferenças nas regiões de 85 e 52 kDa. Estas observações podem oferecer uma explicação a diferenças fisiológicas que ocorrem entre os tripomastigotas sangüícolas e os obtidos de cultura celular.

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ACKNOWLEDGMENTS

This work was supported by Conselho Nacional de Pesquisas, Brasil (Process 40.0695/85). We thank Mr. Carlos Jared for technical assistance and Dr. Rodolfo Pereira Mendes for kindly providing the sera of Chagas' disease patients.

Recebido para publicação em 2/11/1988.

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  • Address for correspondence:
    Dra. Ana Maria Moura da Silva
    Centro de Pesquisa e Formação em Imunologia da OMS/OPS
    Instituto Butantan
    Av. Dr. Vital Brasil, 1500. Caixa Postal 65
    CEP 05504 São Paulo, SP, Brasil

Publication Dates

  • Publication in this collection
    21 Feb 2011
  • Date of issue
    June 1989

History

  • Received
    02 Nov 1988
Instituto de Medicina Tropical de São Paulo Av. Dr. Enéas de Carvalho Aguiar, 470, 05403-000 - São Paulo - SP - Brazil, Tel. +55 11 3061-7005 - São Paulo - SP - Brazil
E-mail: revimtsp@usp.br