Detection of classic and invasive E. coli and Shigella serotypes in stools by indirect immunofluorescence

Dias, Waldely de Oliveira; Pereira, Octávio Augusto de Carvalho

doi:10.1590/S0036-46651984000600002

Abstracts

IIF in the detection of invasive and classic enteropathogenic E. coli and Shigella serotypes was compared with traditional coproculture methods. IIP results agreed with the coproculture findings in 128 out of 140 cases tested for enteropathogenic E. coli (91%) and in 108 out of 112 for Shigella (96%). All cases with positive reactions by coproculture were confirmed by IIP. In the control group it were obtained by IIF 12 cases with positive reactions for enteropathogenic E. coli and 4 cases for Shigella, including two cases of mixed infection by E. coli 026/Sh. dysenteriae and E. coli 0124/Sh. dysenteriae. It was discussed the high sensitivity and specificity of the IIF when compared with the traditional methods, being suggested that IIF is a valuable tool in epidemiological studies involving these organisms and an important aid in the stablishment of an early presumptive diagnosis of the acute infantile diarrhea.

A imunofluorescência indireta (IFI) de sorotipos enteropatogênicos clássicos e invasores de E. coli e de Shigella foi comparada com os métodos tradicionais de coprocultura e soroaglutinação. Os resultados da IFI concordaram com os da coprocultura em 128 dos 140 casos testados para E. coli enteropatogênica (91%) e em 108 dos 112 testados para Shigella (96%). Todos os casos com reações positivas por coprocultura foram confirmados por IFI. No grupo controle, onde não haviam sido isolados tais patógenos por coprocultura, foram evidenciados por IFI, 12 casos com reações positivas para E. coli enteropatogênica e 4 para Shigella, incluindo-se 2 com infecção mista: E. coli 026/Sh. dysenteriae e E. coli 0124/Sh. dysenteriae. Foi discutida a alta sensibilidade e especificidade da IFI quando comparada aos métodos tradicionais, sendo sugerido o valor desta técnica em estudos epidemiológicos envolvendo os microrganismos em questão e sua importância no estabelecimento de diagnóstico precoce na diarréia infantil aguda.

Detection of classic and invasive E. coli and Shigella serotypes in stools by indirect immunofluorescence

Detecção de E. coli enteropatogênica clássica e invasora e Shigella em fezes por imunofluorescência indireta

Waldely de Oliveira Dias; Octávio Augusto de Carvalho Pereira

Escola Paulista de Medicina. Disciplina de Imunologia. Departamento de Microbiologia, Imunologia e Parasitologia Rua Botuoatu, 862 4.° andar. Caixa Postal 7.144 São Paulo, SP Brasil

SUMMARY

IIF in the detection of invasive and classic enteropathogenic E. coli and Shigella serotypes was compared with traditional coproculture methods. IIP results agreed with the coproculture findings in 128 out of 140 cases tested for enteropathogenic E. coli (91%) and in 108 out of 112 for Shigella (96%). All cases with positive reactions by coproculture were confirmed by IIP. In the control group it were obtained by IIF 12 cases with positive reactions for enteropathogenic E. coli and 4 cases for Shigella, including two cases of mixed infection by E. coli 026/Sh. dysenteriae and E. coli 0124/Sh. dysenteriae. It was discussed the high sensitivity and specificity of the IIF when compared with the traditional methods, being suggested that IIF is a valuable tool in epidemiological studies involving these organisms and an important aid in the stablishment of an early presumptive diagnosis of the acute infantile diarrhea.

RESUMO

A imunofluorescência indireta (IFI) de sorotipos enteropatogênicos clássicos e invasores de E. coli e de Shigella foi comparada com os métodos tradicionais de coprocultura e soroaglutinação. Os resultados da IFI concordaram com os da coprocultura em 128 dos 140 casos testados para E. coli enteropatogênica (91%) e em 108 dos 112 testados para Shigella (96%). Todos os casos com reações positivas por coprocultura foram confirmados por IFI. No grupo controle, onde não haviam sido isolados tais patógenos por coprocultura, foram evidenciados por IFI, 12 casos com reações positivas para E. coli enteropatogênica e 4 para Shigella, incluindo-se 2 com infecção mista: E. coli 026/Sh. dysenteriae e E. coli 0124/Sh. dysenteriae. Foi discutida a alta sensibilidade e especificidade da IFI quando comparada aos métodos tradicionais, sendo sugerido o valor desta técnica em estudos epidemiológicos envolvendo os microrganismos em questão e sua importância no estabelecimento de diagnóstico precoce na diarréia infantil aguda.

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Recebido para publicação em 10/2/1984.

Este trabalho recebeu auxílio financeiro da FAPESP e CNPq

1. BORIS, M.; THOMASON, B. M.; HINES, V. D.; MONTAGUE, T. S. & SELLERS, T. F. A community epidemic of enteropathogenic Escherichia coli 0126: B16-.NM gastroenteritis associated with asymptomatic respiratory infection. Pediatrics 33: 18-29, 1964.
2. CARVER, R. K. & GOLDMAN, M. Staining Toxoplasma gondii with fluorescein-labeled antibody. Amer. J. Clin. Path. 32: 159-164, 1959.
3. CHERRY, W. B.; THOMASON, B. M.; POMALES-LEBRÓN, A. & EWING, W. H. Rapid presumptive identification of enteropathogenic Escherichia coli in faecal smears by means of fluorescent antibody. 3. Field evaluation. Bull. WHO 25: 159-171, 1961.
4. CHERRY, W. B. & THOMASON, B. M. Fluorescent antibody technique for Salmonella and other enteric pathogens. Pub. Health Rep. 84: 887-898, 1969.
5. COHEN, F.; PAGE, R. H. & STULBERG, C. Immunofluorescence in diagnostic bacteriology. III. The identification of enteropathogenic E. coli serotypes in fecal smears. Amer. J. Dis. Child. 102: 82-90, 1961.
6. DANIELSSON, D. & LAURELL, G. Identification of enteropathogenic Escherichia coli 0111:B4 by means of fluorescent antibodies. Acta Paediat. 50: 339-345, 1961.
7. DANIELSSON, D.: LAURELL, G. & SJÕLIN, S. An outbreak of diarrhea due to enteropathogenic Escherichia coli studied by means of fluorescent antibody identification and conventional bacteriological culture. Acta Pediat. Scand. 54: 432-438, 1965.
8. DANIELSSON, D. & LAURELL, G. - The fluorescent antibody technique in the diagnosis of enteropathogenic Escherichia coli, with special reference to sensitivity and specificity. Acta Path. Microbiol. 6: 601-610, 1969.
9. EDWARDS, P. R. & EWING, W. H. Identification of Enterobacterial 3rd ed. Minneapolis, Burgess Publishing Co., 1972.
10. FONTES, C. F. Proposição de dois novos meios de cultura e de um sistema simplificado para identificação de enterobactérias. [Tese Mestrado]. São Paulo, Escola Paulista de Medicina, 1979.
11. LINZ, P. & LEJOUR, M. Diagnostic par immunofluorescence des Escherichia coli des gastro-enterites. Pediatric 18: 703-707, 1963.
12. MARSDEN, H. B.; HYDE, W. A. & BRACEGIRDLE, F. Immunofluorescence in the diagnosis of enteropathogenic Escherichia coli infections. Lancet 1: 189-191, 1965.
13. MARTIN, A. J. & O'BRIEN, M. Detection of enteropathogenic Escherichia coli in fecal cultures by use of a modified fluorescent-antibody technique. J. Bact. 89: 570-572, 1965.
14. NELSON, J. D. & WHITAKER, J. A. Diagnosis of enteropathogenic E. coli diarrhea by fluorescein-Iabeled antibodies. J. Pediat. 57 : 684-688, 1960.
15. NELSON, J. D.; WHITAKER, J. A.; HEMPSTEAD, B. & HARRIS, M. Epidemiological application of the fluorescent antibody technique. Study of a diarrhea outbreak in a premature nursery. J.A.M.A. 176: 110-114, 1961.
16. PAGE, R. H. & STULBERG, C. S. Immunofluorescence in epidemiologic control of E. coli diarrhea. Am. J. Dis. Child. 104: 149-156, 1962.
17. SHAUGHNESSY, H. J. et al. An extensive community outbreak of diarrhea due to enteropathogenic Escherichia coli 0111:B4. II. A comparative study of fluorescent antibody identification and standard bacteriological methods. Amer. J. Hyg. 76: 44-51, 1962.
18. TAYLOR, C. E. D.; LEA, D. J.; HEIMER, G. V. & TOMLINSON, A. J. H. Fluorescent antibody techniques in diagnostic bacteriology. Proc. Roy. Soc. Med. 56: 478, 1963.
19. TAYLOR, C. E. D.; HEIMER, G. V.; LEA, D. J. & TOMLINSON, A. J. H. A comparison of a fluorescent antibody technique with a cultural method in the detection of infections with Shigella sonnei. J. Clin. Path. 17: 225-230, 1964.
20. TAYLOR, C. E. D. -& HEIMER, G. V. Rapid diagnosis of sonne dysentery by means of immunofluorescence. Brit. Med. J. 2: 165-166, 1964.
21. THOMASON, B. M.; COWART, G. S. & CHERRY, W. R. Current status of immunofluorescence techniques for rapid detection of Shigella in fecal specimens. Appl. Microbiol. 13: 605-613, 1965.
22. TRABULSI, L. R. & CAMARGO, M. E. Comparative study between immunofluorescence and coproculture in the diagnosis of intestinal infections by enteropathogenic Escherichia coli. Rev. Inst. Med. trop. São Paulo 7: 65-71, 1965.
23. WHEELER, W. E. Infantile diarrhea due to enteropathogenic Escherichia coli In: Trop. F. H. Communicable and Infectious Diseases. Ed. 4. St. Louis, C.V. Mosby Company, 483-494, 1960.
24. WHITAKER, J. A.; PAGE, R. H.; STULBERG, C. S. & ZUELZER, W. W. Rapid identification of enteropathogenic Escherichia coli 0127:B8 by the fluorescent antibody technique. A.M.A. J. Dis. Child. 95: 1-8, 1958.
25. WRAY, C. & THOMLINSON, J. R. The adaptation of a fluorescent antibody staining technique for the quantitative study of potentially pathogenic Escherichia coli in calf faeces. J. Med. Microbiol. 4: 239-248, 1971.

Publication Dates

Publication in this collection
04 Apr 2013
Date of issue
Dec 1984

History

Received
10 Feb 1984

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BORIS, M.; THOMASON, B. M.; HINES, V. D.; MONTAGUE, T. S. & SELLERS, T. F. A community epidemic of enteropathogenic Escherichia coli 0126: B16-.NM gastroenteritis associated with asymptomatic respiratory infection. Pediatrics 33: 18-29, 1964.

[2] 2. CARVER, R. K. & GOLDMAN, M. Staining Toxoplasma gondii with fluorescein-labeled antibody. Amer. J. Clin. Path. 32: 159-164, 1959.

[3] 3. CHERRY, W. B.; THOMASON, B. M.; POMALES-LEBRÓN, A. & EWING, W. H. Rapid presumptive identification of enteropathogenic Escherichia coli in faecal smears by means of fluorescent antibody. 3. Field evaluation. Bull. WHO 25: 159-171, 1961.

[4] 4. CHERRY, W. B. & THOMASON, B. M. Fluorescent antibody technique for Salmonella and other enteric pathogens. Pub. Health Rep. 84: 887-898, 1969.

[5] 5. COHEN, F.; PAGE, R. H. & STULBERG, C. Immunofluorescence in diagnostic bacteriology. III. The identification of enteropathogenic E. coli serotypes in fecal smears. Amer. J. Dis. Child. 102: 82-90, 1961.

[6] 6. DANIELSSON, D. & LAURELL, G. Identification of enteropathogenic Escherichia coli 0111:B4 by means of fluorescent antibodies. Acta Paediat. 50: 339-345, 1961.

[7] 7. DANIELSSON, D.: LAURELL, G. & SJÕLIN, S. An outbreak of diarrhea due to enteropathogenic Escherichia coli studied by means of fluorescent antibody identification and conventional bacteriological culture. Acta Pediat. Scand. 54: 432-438, 1965.

[8] 8. DANIELSSON, D. & LAURELL, G. - The fluorescent antibody technique in the diagnosis of enteropathogenic Escherichia coli, with special reference to sensitivity and specificity. Acta Path. Microbiol. 6: 601-610, 1969.

[9] 9. EDWARDS, P. R. & EWING, W. H. Identification of Enterobacterial 3rd ed. Minneapolis, Burgess Publishing Co., 1972.

[10] 10. FONTES, C. F. Proposição de dois novos meios de cultura e de um sistema simplificado para identificação de enterobactérias. [Tese Mestrado]. São Paulo, Escola Paulista de Medicina, 1979.

[11] 11. LINZ, P. & LEJOUR, M. Diagnostic par immunofluorescence des Escherichia coli des gastro-enterites. Pediatric 18: 703-707, 1963.

[12] 12. MARSDEN, H. B.; HYDE, W. A. & BRACEGIRDLE, F. Immunofluorescence in the diagnosis of enteropathogenic Escherichia coli infections. Lancet 1: 189-191, 1965.

[13] 13. MARTIN, A. J. & O'BRIEN, M. Detection of enteropathogenic Escherichia coli in fecal cultures by use of a modified fluorescent-antibody technique. J. Bact. 89: 570-572, 1965.

[14] 14. NELSON, J. D. & WHITAKER, J. A. Diagnosis of enteropathogenic E. coli diarrhea by fluorescein-Iabeled antibodies. J. Pediat. 57 : 684-688, 1960.

[15] 15. NELSON, J. D.; WHITAKER, J. A.; HEMPSTEAD, B. & HARRIS, M. Epidemiological application of the fluorescent antibody technique. Study of a diarrhea outbreak in a premature nursery. J.A.M.A. 176: 110-114, 1961.

[16] 16. PAGE, R. H. & STULBERG, C. S. Immunofluorescence in epidemiologic control of E. coli diarrhea. Am. J. Dis. Child. 104: 149-156, 1962.

[17] 17. SHAUGHNESSY, H. J. et al. An extensive community outbreak of diarrhea due to enteropathogenic Escherichia coli 0111:B4. II. A comparative study of fluorescent antibody identification and standard bacteriological methods. Amer. J. Hyg. 76: 44-51, 1962.

[18] 18. TAYLOR, C. E. D.; LEA, D. J.; HEIMER, G. V. & TOMLINSON, A. J. H. Fluorescent antibody techniques in diagnostic bacteriology. Proc. Roy. Soc. Med. 56: 478, 1963.

[19] 19. TAYLOR, C. E. D.; HEIMER, G. V.; LEA, D. J. & TOMLINSON, A. J. H. A comparison of a fluorescent antibody technique with a cultural method in the detection of infections with Shigella sonnei. J. Clin. Path. 17: 225-230, 1964.

[20] 20. TAYLOR, C. E. D. -& HEIMER, G. V. Rapid diagnosis of sonne dysentery by means of immunofluorescence. Brit. Med. J. 2: 165-166, 1964.

[21] 21. THOMASON, B. M.; COWART, G. S. & CHERRY, W. R. Current status of immunofluorescence techniques for rapid detection of Shigella in fecal specimens. Appl. Microbiol. 13: 605-613, 1965.

[22] 22. TRABULSI, L. R. & CAMARGO, M. E. Comparative study between immunofluorescence and coproculture in the diagnosis of intestinal infections by enteropathogenic Escherichia coli. Rev. Inst. Med. trop. São Paulo 7: 65-71, 1965.

[23] 23. WHEELER, W. E. Infantile diarrhea due to enteropathogenic Escherichia coli In: Trop. F. H. Communicable and Infectious Diseases. Ed. 4. St. Louis, C.V. Mosby Company, 483-494, 1960.

[24] 24. WHITAKER, J. A.; PAGE, R. H.; STULBERG, C. S. & ZUELZER, W. W. Rapid identification of enteropathogenic Escherichia coli 0127:B8 by the fluorescent antibody technique. A.M.A. J. Dis. Child. 95: 1-8, 1958.

[25] 25. WRAY, C. & THOMLINSON, J. R. The adaptation of a fluorescent antibody staining technique for the quantitative study of potentially pathogenic Escherichia coli in calf faeces. J. Med. Microbiol. 4: 239-248, 1971.

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Detection of classic and invasive E. coli and Shigella serotypes in stools by indirect immunofluorescence

Detecção de E. coli enteropatogênica clássica e invasora e Shigella em fezes por imunofluorescência indireta

Abstracts

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History