Use of MAC-ELISA for evaluation of yellow fever vaccination

Nogueira, Rita M. Ribeiro; Schatzmayr, Hermann G.; Miagostovich, Marize P.; Cavalcanti, Silvia M. B.; Carvalho, Ricardo de

doi:10.1590/S0036-46651992000500012

Abstracts

An evaluation of the IgM antibody immune response against yellow fever using strain 17D was carried out by MAC-ELISA and PRNT. The results showed an agreement of 97% between both tests and the authors conclude that MAC-ELISA can be used as a specific and sensitive asssay to replace the PRNT for detecting yellow fever antibodies in human sera, after vaccination programs.

Yellow fever vaccinated; MAC-ELISA; PRNT

Foi avaliada a resposta imune de anticorpos IgM em vacinados contra febre amarela usando a amostra 17D empregando-se provas de captura de anticorpos IgM (MAC-ELISA) e teste de neutralização por redução de placas (PRNT). Os resulta-dos mostraram uma concordância de 97% entre ambas as provas e os autores concluem que o MAC-ELISA pode ser utilizado como um método específico e sensível para substituir o teste de neutralização para detecção de anticorpos IgM em vacinados contra febre amarela.

SEROLOGY

Use of MAC-ELISA for evaluation of yellow fever vaccination

Uso do MAC-ELISA para avaliação de vacinados contra febre amarela

Rita M. Ribeiro Nogueira^I; Hermann G. Schatzmayr^I; Marize P. Miagostovich^I; Silvia M. B. Cavalcanti^I; Ricardo de Carvalho^II

^IInstituto Oswaldo Cruz, Departamento de Virologia, Fundação Oswaldo Cruz, Caixa Postal 926, 21040 Rio de Janeiro, RJ, Brasil

^IIBio-Manguinhos, Fundação Oswaldo Cruz

SUMMARY

An evaluation of the IgM antibody immune response against yellow fever using strain 17D was carried out by MAC-ELISA and PRNT. The results showed an agreement of 97% between both tests and the authors conclude that MAC-ELISA can be used as a specific and sensitive asssay to replace the PRNT for detecting yellow fever antibodies in human sera, after vaccination programs.

Key words: Yellow fever vaccinated; MAC-ELISA; PRNT

RESUMO

Foi avaliada a resposta imune de anticorpos IgM em vacinados contra febre amarela usando a amostra 17D empregando-se provas de captura de anticorpos IgM (MAC-ELISA) e teste de neutralização por redução de placas (PRNT). Os resulta-dos mostraram uma concordância de 97% entre ambas as provas e os autores concluem que o MAC-ELISA pode ser utilizado como um método específico e sensível para substituir o teste de neutralização para detecção de anticorpos IgM em vacinados contra febre amarela.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

ACKNOWLEDGEMENTS

The authors thank Dr. D.J. Gubler from CDC for yellow fever antigens and Dra. Luiza Therezinha M. Souza from Adolfo Lutz Institute for Rocio antigens supply. The authors also thank Dr. O. S. Lopes ("In memorian") for providing sera from military personnel and encouragement .

Recebido para publicação em 6/1/1992

Aceito para publicação em 15/5/1992

1. BUNDO, K. & IGARASHI, A. - Antibody - capture Elisa for detection of immunoglobulin M antibodies in sera from Japanese Encephalitis and dengue hemorrhagic fever patients. J. virol. Meth., 11: 15-22, 1985.
2. BURKE, D.S. & NISALAK, A. - Detection of Japanese Encephalitis virus immunoglobulin M antibodies in serum by antibody capture radioimmunoassay. J. clin. Microbiol., 15: 353-361, 1982.
3. CLARKE, D.H. & CASALS, J. - Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Amer. J. trop. Med. Hyg., 7: 561-573, 1958.
4. KUNO, G.; GOMEZ, I. & GUBLER, D.J. - Detecting artificial anti-dengue IgM immune complexes using an enzyme linked immunosorbent assay. Amer. J. trop. Med. Hyg., 36: 153-159, 1987.
5. LHUILLIER, M.; SARTHOU, J.L.; CORDELLIER, R.; MONTENY, N.; GERSHY-DAMET, G.M. & BOUCHITE, B. - Emergence endémique de la fičvre jaune en Côte d'Ivoire: place de la détection des IgM antiamariles dans la stratégie de surveillance. Bull. Org. mond. Santé, 64: 415-420, 1986.
6. LOPES, O.S.; COIMBRA, T.L.M.; SACCHETTA, L.A. & CALISHER, C.H. - Emergence of a new arbovirus disease in Brazil. I. Isolation and characterization of the etiologic agent, Rocio virus. Amer. J. Epidem., 107: 444-449, 1978.
7. LOPES, O.S.; GUIMARĂES, S.S.D.A. & CARVALHO, R. - Studies on yellow fever vaccine. DL Dose response in volunteers. J. biol. Stand., 16: 77-82, 1988.
8. MONATH, T.P. & NISTRON, R.R. - Detection of yellow fever virus in serum by enzyme immunoassay. Amer. J. trop. Med. Hyg., 33: 151-157, 1984.
9. NOGUEIRA, R.M.R.; SCHATZMAYR, H.G.; MIAGOSTOVICH, M.P.; FARIAS, M.F.D.B. & FARIAS FILHO, J.C. - Virological study of a dengue type 1 epidemic at Rio de Janeiro. Mem. Inst. Oswaldo Cruz., 83: 219-225, 1988.
10. OKAMOTO, Y.; OKUNO, Y.; YAMADA, A.; BABA, K. & YABUUCHI, H. - Enzyme linked immunosorbent assay for detection of serum antibody in children vaccinated with Japanese encephalitis vaccine. Biken's J., 29: 57-62, 1986.
11. SALLUZO, J.F.; SARTHOU, J.L.; CORNET, M.; DIGOUTTE, J.P. & MONATH, T.P. - Intérčt du titrage par Elisa des IgM spécifiques pour la diagnostic et la surveillance de la circulation selvatique des flavivirus en Afrique. Ann. Inst. Pasteur Virol., 137 E: 155-170, 1986.
12. SCHATZMAYR, H.G.; BARTH, O.M. & ALENCAR, A.A. - Demonstraçăo por imunofluorescęncia direta de antígenos do vírus da febre amarela em tecido hepático pré-tratado com tripsina. Mem. Inst. Oswaldo Cruz, 79: 93-99, 1984.
13. SCHATZMAYR, H.G.; NOGUEIRA, R.M.R. & TRAVASSOS DA ROSA, A.P.A. - An outbreak of dengue at Rio de Janeiro - 1986. Mem. Inst. Oswaldo Cruz, 81: 245-246, 1986.
14. VOLLER, A.; BARTLETT, A. & BIDWELL, D.E. - Enzyme immunoassays with special reference to Elisa techniques. J. clin. Path., 31: 507-520, 1978.
15. YAMADA, A.; IMANISHI, J.; JUANG, R.F.; FUKUNAGA, T.; OKUNO, Y.; TADANO, M.; FUKAI, K. & YABUUCHI, H. - Trial of inactivated Japanese Encephalitis vaccine in children with underlying disease. Vaccine, 4: 32-34, 1986.

Publication Dates

Publication in this collection
13 Sept 2006
Date of issue
Oct 1992

History

Received
06 Jan 1992
Accepted
15 May 1992

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BUNDO, K. & IGARASHI, A. - Antibody - capture Elisa for detection of immunoglobulin M antibodies in sera from Japanese Encephalitis and dengue hemorrhagic fever patients. J. virol. Meth., 11: 15-22, 1985.

[2] 2. BURKE, D.S. & NISALAK, A. - Detection of Japanese Encephalitis virus immunoglobulin M antibodies in serum by antibody capture radioimmunoassay. J. clin. Microbiol., 15: 353-361, 1982.

[3] 3. CLARKE, D.H. & CASALS, J. - Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Amer. J. trop. Med. Hyg., 7: 561-573, 1958.

[4] 4. KUNO, G.; GOMEZ, I. & GUBLER, D.J. - Detecting artificial anti-dengue IgM immune complexes using an enzyme linked immunosorbent assay. Amer. J. trop. Med. Hyg., 36: 153-159, 1987.

[5] 5. LHUILLIER, M.; SARTHOU, J.L.; CORDELLIER, R.; MONTENY, N.; GERSHY-DAMET, G.M. & BOUCHITE, B. - Emergence endémique de la fičvre jaune en Côte d'Ivoire: place de la détection des IgM antiamariles dans la stratégie de surveillance. Bull. Org. mond. Santé, 64: 415-420, 1986.

[6] 6. LOPES, O.S.; COIMBRA, T.L.M.; SACCHETTA, L.A. & CALISHER, C.H. - Emergence of a new arbovirus disease in Brazil. I. Isolation and characterization of the etiologic agent, Rocio virus. Amer. J. Epidem., 107: 444-449, 1978.

[7] 7. LOPES, O.S.; GUIMARĂES, S.S.D.A. & CARVALHO, R. - Studies on yellow fever vaccine. DL Dose response in volunteers. J. biol. Stand., 16: 77-82, 1988.

[8] 8. MONATH, T.P. & NISTRON, R.R. - Detection of yellow fever virus in serum by enzyme immunoassay. Amer. J. trop. Med. Hyg., 33: 151-157, 1984.

[9] 9. NOGUEIRA, R.M.R.; SCHATZMAYR, H.G.; MIAGOSTOVICH, M.P.; FARIAS, M.F.D.B. & FARIAS FILHO, J.C. - Virological study of a dengue type 1 epidemic at Rio de Janeiro. Mem. Inst. Oswaldo Cruz., 83: 219-225, 1988.

[10] 10. OKAMOTO, Y.; OKUNO, Y.; YAMADA, A.; BABA, K. & YABUUCHI, H. - Enzyme linked immunosorbent assay for detection of serum antibody in children vaccinated with Japanese encephalitis vaccine. Biken's J., 29: 57-62, 1986.

[11] 11. SALLUZO, J.F.; SARTHOU, J.L.; CORNET, M.; DIGOUTTE, J.P. & MONATH, T.P. - Intérčt du titrage par Elisa des IgM spécifiques pour la diagnostic et la surveillance de la circulation selvatique des flavivirus en Afrique. Ann. Inst. Pasteur Virol., 137 E: 155-170, 1986.

[12] 12. SCHATZMAYR, H.G.; BARTH, O.M. & ALENCAR, A.A. - Demonstraçăo por imunofluorescęncia direta de antígenos do vírus da febre amarela em tecido hepático pré-tratado com tripsina. Mem. Inst. Oswaldo Cruz, 79: 93-99, 1984.

[13] 13. SCHATZMAYR, H.G.; NOGUEIRA, R.M.R. & TRAVASSOS DA ROSA, A.P.A. - An outbreak of dengue at Rio de Janeiro - 1986. Mem. Inst. Oswaldo Cruz, 81: 245-246, 1986.

[14] 14. VOLLER, A.; BARTLETT, A. & BIDWELL, D.E. - Enzyme immunoassays with special reference to Elisa techniques. J. clin. Path., 31: 507-520, 1978.

[15] 15. YAMADA, A.; IMANISHI, J.; JUANG, R.F.; FUKUNAGA, T.; OKUNO, Y.; TADANO, M.; FUKAI, K. & YABUUCHI, H. - Trial of inactivated Japanese Encephalitis vaccine in children with underlying disease. Vaccine, 4: 32-34, 1986.