Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensis

Masuda, Aoi; Nascimento, Sueli Fátima do; Guerra, Carmem Silvia; Paranhos, Gláucia da Silva; Ferreira, Antonio Walter

doi:10.1590/S0036-46651989000400004

Abstracts

The antigenicity of promastigotes of Leishmania braziliensis braziliensis (L. b.braziliensis) treated with 1% sodium desoxycholate in 10 mM Tris-Hcl pH 8.2 was analysed by immunoblot using as probes sera from American cutaneous leishmaniasis (ACL), American visceral leishmaniasis (AVL), schistosomiasis, malaria and Chagas' disease. The ACL sera reacted constantly with a 60 kD band. No reactivity to this protein was observed with sera from the other diseases above mentioned indicating that the 60 kD protein may be used in serodiagnosis for ACL.

Antigens of Leishmania braziliensis braziliensis; Serodiagnosis of American cutaneous leishmaniasis

A antigenicidade de promastigotas de Leishmania braziliensis braziliensis tratadas com desoxicolato de sódio 1% tampão TrisHCl 10 mM pH 8.2 foi determinada por immunoblot usando soros de pacientes com leishmaniose cutânea e mucocutânea, leishmaniose visceral, esquistossomose, malária e doença de Chagas. Os soros de pacientes com leishmaniose cutânea e mucocutânea apresentaram reação positiva com uma banda de 60 kD. Não se observou reatividade para esta fração em soros de pacientes com outras doenças parasitárias acima mencionadas, indicando que esta pode ser utilizada no sorodiagnóstico de leishmaniose tegumental.

ORIGINAL ARTICLES

Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensis

Análise da especificidade de anticorpos humanos a antígenos de Leishmania braziliensis braziliensis

Aoi Masuda; Sueli Fátima do Nascimento; Carmem Silvia Guerra; Gláucia da Silva Paranhos; Antonio Walter Ferreira

Laboratório de Sorologia e Imunologia do Instituto de Medicina Tropical de São Paulo, SP, Brasil

^{Address for correspondence} Address for correspondence: Dr. Antonio Walter Ferreira Instituto de Medicina Tropical de São Paulo Av. Dr. Enéas de Carvalho Aguiar, 470 CEP 05403 São Paulo, SP, Brasil

SUMMARY

The antigenicity of promastigotes of Leishmania braziliensis braziliensis (L. b.braziliensis) treated with 1% sodium desoxycholate in 10 mM Tris-Hcl pH 8.2 was analysed by immunoblot using as probes sera from American cutaneous leishmaniasis (ACL), American visceral leishmaniasis (AVL), schistosomiasis, malaria and Chagas' disease. The ACL sera reacted constantly with a 60 kD band. No reactivity to this protein was observed with sera from the other diseases above mentioned indicating that the 60 kD protein may be used in serodiagnosis for ACL.

Key words:Antigens of Leishmania braziliensis braziliensis; Serodiagnosis of American cutaneous leishmaniasis.

RESUMO

A antigenicidade de promastigotas de Leishmania braziliensis braziliensis tratadas com desoxicolato de sódio 1% tampão TrisHCl 10 mM pH 8.2 foi determinada por immunoblot usando soros de pacientes com leishmaniose cutânea e mucocutânea, leishmaniose visceral, esquistossomose, malária e doença de Chagas. Os soros de pacientes com leishmaniose cutânea e mucocutânea apresentaram reação positiva com uma banda de 60 kD. Não se observou reatividade para esta fração em soros de pacientes com outras doenças parasitárias acima mencionadas, indicando que esta pode ser utilizada no sorodiagnóstico de leishmaniose tegumental.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

ACKNOWLEDGEMENTS

The authors greatefully acknowledge Dr. Roberto Badaró for kindly provinding sera of patients with specific diagnosis of leishmaniasis caused by L. b. braziliensis. Paulo de Oliveira and Rivaldo de Souza Cardoso for the excellent Technical assistance and Ceei Corrêa Godoi de Angelis and Eunice Bonfim Pinto for editorial assistance.

This work was partially supported by Fundação de Amparo à Pesquisa do Estado de São Paulo - 86/2389, by the UNDP/WORLD/ BANK/WHO Special Programme for Research and Training in Tropical Diseases (ID - 870 250); by Laboratório de Investigação Médica - HC-FMUSP - (LIM-48); and by FINEP (43.88.0689/00).

Recebido para publicação em 1/2/1989.

1. ARNOT, D.E. & BARKER, D.C. - Biochemical identification of cutaneous leishmanias by analysis of kinetoplastic DNA: II Sequence homologies in Leishmania KDNA. Molec. Biochem. Parasit., 3:47-56, 1981.
2. BADARO, R.; REED, S.G.; BARRAL, A.; ORGE, G. & JONES, T.C. - Evaluation of the microenzymelinked immunosorbent assay (ELISA) for antibodies in American visceral leishmaniasis: Antigen selection for detection of infection-specific responses. Amer. J. trop. Med. Hyg., 35:72-78, 1986.
3. BARKER, D.C; GIBSON, L.J.; KENNEDY, W.P.K.; NASSER, A.A.A.A. & WILLIAMS, R.H. - The potential of using recombinant DNA species-specific probes for the identification of tropical Leishmania. Parasitology, 91:S139-S174, 1986.
4. BORDIER, C. - The promastigotes surface protease of Leishmania. Parasit. today, 3:151-153, 1987.
5. CHANCE, H.L.; PETERS, W. & SCHORY, L. - Biochemical taxonomy of Leishmania: I observation of DNA. Ann. trop. Med. Parasit., 68:307-316, 1974.
6. DEANE, K.M. & GRIMALDI JR, G. - Leishmaniasis in Brazil. In: CHANG, K.P. & BRAY, R.S., ed. - Leishmaniasis. Amsterdam, Elsevier, 1985. p.247-281. (Human Parasitic Diseases, v.1).
7. GARDNER, P.J.; CHANCE, M.L. & PETERS, W. - Biochemical taxonomy of Leishmania: II Electrophoretic variation of malate dehydrogenase. Ann. trop. Med. Parasit., 68:307-328, 1974.
8. HENDRICKS, L.D. & WRIGHT, N. - Diagnosis of cutaneous leishmaniasis by in vitro cultivation of saline aspirates in Schneider's Drosophila Medium. Amer. J. trop. Med. Hyg., 28:962-964, 1979.
9. KOHANTEB, J.; ARDEHALI, S. & REED, H.R. - Studies on antigenic relationship of Leishmania promastigotes by electroimmunodiffusion and crossed electroimmunodiffusion tests. Trans, roy. Soc. trop. Med. Hyg., 74:582-584, 1980.
10. LAEMMLI, V.K. - Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227:680-685, 1970.
11. LEGRAND, D.; DESJEAUX, P.; LE PONT, F.; KAHANE, B. & BRENIÈRE, S.F. - Evidence for a 72 kD specific surface in Leishmania braziliensis braziliensis. Mem. Inst. Oswaldo Cruz, 81(supl.):90, 1986.
12. LOWRY, O.H.; ROSENBROUGH, M.J.; FARR, A.L. & RANDALL, R.J. - Protein measurement with the folin phenol reagent. J. biol. Chem., 193:265-275, 1951.
13. MACMAHON-PRATT, D.; BENNET, E. & DAVID, J.R. - Monoclonal antibodies that distinguish subspecies of Leishmania braziliensis braziliensis. J. Immunol., 129:926-927, 1982.
14. MACMAHON-PRATT, D. & DAVID, J.R. - Monoclonal antibodies that distinguish between New World species of Leishmania. Nature, 291:581-583, 1981.
15. MILES, M.A.; POVOA, M.M.; DE SOUZA, A.A.; LAINSON, R. & SHAW, J.J. - Some methods for the enzymatic characterization of Latin-American Leishmania with particular reference to Leishmania mexicana amazonensis and subspecies of Leishmania hertigi. Trans, roy. Soc. trop. Med. Hyg., 74:243-252, 1979.
16. MITCHELL, G.F. & HANDMAN, E. - The glycoconjugate derived from a Leishmania major receptor for macrophages is a suppressogenic disease promoting antigen in murine cutaneous leishmaniasis. Parasite Immunol., 8:255-263, 1986.
17. SANTOS, J.L, MORGADO, M.G. & GALVÃO, B.. - Human visceral leishmaniasis: Analysis of the specificity of humoral immune response to polypeptides of Leishmania donovam chagasi. Amer. J. trop. Med. Hyg., 37:263-277, 1987.
18. TOWBIN, H.; STAEHELIN, T. & BORDON, J. - Transfer of proteins from polyacrylamide gels to nitrocellulose sheets procedure of some applications. Proc. nat. Acad. Sci. (Wash), 76:4350-4354, 1979.
19. ULRICH, M.; CENTENO, M.; MATTOUT, Z. & CONVIT, J. - Serological patterns and specificity in American cutaneous leishmaniasis. Amer. J. trop. Med. Hyg., 39:179-184, 1988.

Address for correspondence:

Dr. Antonio Walter Ferreira

Instituto de Medicina Tropical de São Paulo

Av. Dr. Enéas de Carvalho Aguiar, 470

CEP 05403 São Paulo, SP, Brasil

Publication Dates

Publication in this collection
14 Oct 2011
Date of issue
Aug 1989

History

Received
01 Feb 1989

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ARNOT, D.E. & BARKER, D.C. - Biochemical identification of cutaneous leishmanias by analysis of kinetoplastic DNA: II Sequence homologies in Leishmania KDNA. Molec. Biochem. Parasit., 3:47-56, 1981.

[2] 2. BADARO, R.; REED, S.G.; BARRAL, A.; ORGE, G. & JONES, T.C. - Evaluation of the microenzymelinked immunosorbent assay (ELISA) for antibodies in American visceral leishmaniasis: Antigen selection for detection of infection-specific responses. Amer. J. trop. Med. Hyg., 35:72-78, 1986.

[3] 3. BARKER, D.C; GIBSON, L.J.; KENNEDY, W.P.K.; NASSER, A.A.A.A. & WILLIAMS, R.H. - The potential of using recombinant DNA species-specific probes for the identification of tropical Leishmania. Parasitology, 91:S139-S174, 1986.

[4] 4. BORDIER, C. - The promastigotes surface protease of Leishmania. Parasit. today, 3:151-153, 1987.

[5] 5. CHANCE, H.L.; PETERS, W. & SCHORY, L. - Biochemical taxonomy of Leishmania: I observation of DNA. Ann. trop. Med. Parasit., 68:307-316, 1974.

[6] 6. DEANE, K.M. & GRIMALDI JR, G. - Leishmaniasis in Brazil. In: CHANG, K.P. & BRAY, R.S., ed. - Leishmaniasis. Amsterdam, Elsevier, 1985. p.247-281. (Human Parasitic Diseases, v.1).

[7] 7. GARDNER, P.J.; CHANCE, M.L. & PETERS, W. - Biochemical taxonomy of Leishmania: II Electrophoretic variation of malate dehydrogenase. Ann. trop. Med. Parasit., 68:307-328, 1974.

[8] 8. HENDRICKS, L.D. & WRIGHT, N. - Diagnosis of cutaneous leishmaniasis by in vitro cultivation of saline aspirates in Schneider's Drosophila Medium. Amer. J. trop. Med. Hyg., 28:962-964, 1979.

[9] 9. KOHANTEB, J.; ARDEHALI, S. & REED, H.R. - Studies on antigenic relationship of Leishmania promastigotes by electroimmunodiffusion and crossed electroimmunodiffusion tests. Trans, roy. Soc. trop. Med. Hyg., 74:582-584, 1980.

[10] 10. LAEMMLI, V.K. - Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227:680-685, 1970.

[11] 11. LEGRAND, D.; DESJEAUX, P.; LE PONT, F.; KAHANE, B. & BRENIÈRE, S.F. - Evidence for a 72 kD specific surface in Leishmania braziliensis braziliensis. Mem. Inst. Oswaldo Cruz, 81(supl.):90, 1986.

[12] 12. LOWRY, O.H.; ROSENBROUGH, M.J.; FARR, A.L. & RANDALL, R.J. - Protein measurement with the folin phenol reagent. J. biol. Chem., 193:265-275, 1951.

[13] 13. MACMAHON-PRATT, D.; BENNET, E. & DAVID, J.R. - Monoclonal antibodies that distinguish subspecies of Leishmania braziliensis braziliensis. J. Immunol., 129:926-927, 1982.

[14] 14. MACMAHON-PRATT, D. & DAVID, J.R. - Monoclonal antibodies that distinguish between New World species of Leishmania. Nature, 291:581-583, 1981.

[15] 15. MILES, M.A.; POVOA, M.M.; DE SOUZA, A.A.; LAINSON, R. & SHAW, J.J. - Some methods for the enzymatic characterization of Latin-American Leishmania with particular reference to Leishmania mexicana amazonensis and subspecies of Leishmania hertigi. Trans, roy. Soc. trop. Med. Hyg., 74:243-252, 1979.

[16] 16. MITCHELL, G.F. & HANDMAN, E. - The glycoconjugate derived from a Leishmania major receptor for macrophages is a suppressogenic disease promoting antigen in murine cutaneous leishmaniasis. Parasite Immunol., 8:255-263, 1986.

[17] 17. SANTOS, J.L, MORGADO, M.G. & GALVÃO, B.. - Human visceral leishmaniasis: Analysis of the specificity of humoral immune response to polypeptides of Leishmania donovam chagasi. Amer. J. trop. Med. Hyg., 37:263-277, 1987.

[18] 18. TOWBIN, H.; STAEHELIN, T. & BORDON, J. - Transfer of proteins from polyacrylamide gels to nitrocellulose sheets procedure of some applications. Proc. nat. Acad. Sci. (Wash), 76:4350-4354, 1979.

[19] 19. ULRICH, M.; CENTENO, M.; MATTOUT, Z. & CONVIT, J. - Serological patterns and specificity in American cutaneous leishmaniasis. Amer. J. trop. Med. Hyg., 39:179-184, 1988.