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Entamoeba histolytica: antigenic characterization of axenic strains from Brazil

Entamoeba histolytica: caracterização antigênica de cepas axênicas do Brasil

Abstracts

Trophozoites from cultures of Entamoeba histolytica strains isolated and grown axenically in Brazil (ICB-CSP, ICB-462 and ICB-32) were used for immune sera production and for characterization of their antigens by using electrophoretic and glycoproteic profiles, in parallel with a standard strain isolated and kept under axenic conditions in USA (HK-9). Hyperimmune sera, presenting high antibody titers with homologous and heterologous antigens, were obtained. The four strains in study revealed similar and complex electrophoretic and glycoproteic profiles showing polypep-tides with molecular weights ranging from 200 to less than 29 kDa. No significant differences were detected between the pathogenic and non-pathogenic strains.

Entamoeba histolytica; Axenic strains; Antigens


Trofozoítos obtidos de cultura de Entamoeba histolytica isoladas e axenizadas no Brasil (ICB-CSP, ICB-462 e ICB-32) foram utilizados para a produção de soros imunes em coelhos e para a caracterização de antígenos através dos seus perfis eletroforético e glicoproteico, em paralelo com uma cepa padrão isolada e axenizada nos Estados Unidos (HK-9). Obtiveram-se soros hiperimunes, reativos frente aos antígenos homólogos e heterólogos. As quatro cepas em estudo apresentaram perfis eletroforético e glicoproteico complexos e semelhantes, compostos por polipeptídeos com pesos moleculares variando de 200 kDa a menos de 29 kDa. Não se detectaram diferenças significativas entre as cepas patogênicas e não patogênicas.


ORIGINAL ARTICLES

Entamoeba histolytica: antigenic characterization of axenic strains from Brazil

Entamoeba histolytica: caracterização antigênica de cepas axênicas do Brasil

Semíramis GuimarãesI; Haidée UrdanetaII; Edward F. SilvaI; Carlos Alberto P. TavaresIII

IDepartment of Parasitology, Institute of Biological Science, Federal Minas Gerais University. Belo Horizonte, MG, Brazil

IIDepartment of Biology, Los Andes University, Venezuela

IIIDepartment of Biochemistry and Immunology, Institute of Biological Science, Federal Minas Gerais University. Belo Horizonte, MG, Brazil

Address for correspondence Address for correspondence: Dr. Carlos Alberto P. Tavares Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais Caixa Postal 2486 CEP 30161 Belo Horizonte, MG, Brasil

SUMMARY

Trophozoites from cultures of Entamoeba histolytica strains isolated and grown axenically in Brazil (ICB-CSP, ICB-462 and ICB-32) were used for immune sera production and for characterization of their antigens by using electrophoretic and glycoproteic profiles, in parallel with a standard strain isolated and kept under axenic conditions in USA (HK-9). Hyperimmune sera, presenting high antibody titers with homologous and heterologous antigens, were obtained. The four strains in study revealed similar and complex electrophoretic and glycoproteic profiles showing polypep-tides with molecular weights ranging from 200 to less than 29 kDa. No significant differences were detected between the pathogenic and non-pathogenic strains.

Key words: Entamoeba histolytica; Axenic strains; Antigens.

RESUMO

Trofozoítos obtidos de cultura de Entamoeba histolytica isoladas e axenizadas no Brasil (ICB-CSP, ICB-462 e ICB-32) foram utilizados para a produção de soros imunes em coelhos e para a caracterização de antígenos através dos seus perfis eletroforético e glicoproteico, em paralelo com uma cepa padrão isolada e axenizada nos Estados Unidos (HK-9). Obtiveram-se soros hiperimunes, reativos frente aos antígenos homólogos e heterólogos. As quatro cepas em estudo apresentaram perfis eletroforético e glicoproteico complexos e semelhantes, compostos por polipeptídeos com pesos moleculares variando de 200 kDa a menos de 29 kDa. Não se detectaram diferenças significativas entre as cepas patogênicas e não patogênicas.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

ACKNOWLEDGMENTS

We particularlly thank Dr. Antoniana Ursine Krettli for her critical review of this work. We also thank Mr. João da Costa Viana, Ms. Marinete L. Ludgero and Ms. Edna Pires for their technical assistance. This work was supported by CNPq, FINEP and FAPEMIG.

Recebido para publicação em 14/3/1990.

Aceito para publicação em 30/10/1990.

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  • Address for correspondence:

    Dr. Carlos Alberto P. Tavares
    Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais
    Caixa Postal 2486
    CEP 30161 Belo Horizonte, MG, Brasil
  • Publication Dates

    • Publication in this collection
      12 Sept 2006
    • Date of issue
      Feb 1991

    History

    • Accepted
      30 Oct 1990
    • Received
      14 Mar 1990
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