Fascioliasis in relatives of patients with Fasciola hepatica infection in Peru

Fasciolosis en familiares de pacientes con infección por Fasciola hepatica en el Perú

Abstracts

High prevalence rates of human fascioliasis have been described in several regions of Peru. We surveyed 20 families in an endemic area of Peru in order to determine the proportion of infection with F. hepatica in relatives of diagnosed subjects and in order to identify associated risk factors. The study included feces and blood samples of 93 subjects. Ages ranged from one to 53 (mean = 18.6; SD = 14.2). The overall prevalence of fascioliasis by fecal examinations was 33.3% (n = 83) and by serology, 51.9% (n = 86). The prevalence in age group I (< 19 years old) by coprological and serological tests was 61.4% and 75.9%, respectively; in group II (> 19 years old) 15.4% and 37.5%. The main associated risk factor with fascioliasis was eating salads (OR = 3.29, CI = 1.2 - 9.0, p = 0.02). In conclusion, human fascioliasis is highly prevalent in the relatives of index cases and the most significant risk factor of acquiring fascioliasis in the family is eating salads in endemic areas.

Fascioliasis; Relatives; Index case; Risk factors; Salads; Peru


Altas tasas de fasciolosis humana han sido descritas en varias regiones del Perú. Estudiamos 20 familias en una área endémica del Perú para determinar la proporción de infección con F. hepatica en los familiares de los sujetos diagnosticados y para identificar factores de riesgo asociados. El estudio incluyó un total de 93 sujetos, quienes contribuyeron con muestras de heces y sangre. Las edades comprendieron desde 1 a 53 años (media = 18.6; DS = 14.2). La prevalencia general de fasciolosis por exámenes de heces fue 33.3% (n = 83) y por serología, 51.9% (n = 86). La prevalencia en el grupo de edad I (< 19 años de edad) por pruebas coprológicas y serológicas fueron 61.4% y 75.9%, respectivamente; en el grupo II (> 19 años de edad) 15.4% y 37.5%. El principal factor de riesgo asociado con fasciolosis fue comer ensaladas (OR = 3.29, IC = 1.2-9.0, p = 0.02). En conclusión, la fasciolosis humana es altamente prevalente en familiares de los casos índices y el factor de riesgo más significante para adquirirla en la familia es comer ensaladas en las áreas endémicas.


PARASITOLOGY

Fascioliasis in relatives of patients with Fasciola hepatica infection in Peru

Fasciolosis en familiares de pacientes con infección por Fasciola hepatica en el Perú

Luis MarcosI; Vicente MacoI; Angélica TerashimaI; Frine SamalvidesI; José R. EspinozaII; Eduardo GotuzzoI

IInstituto de Medicina Tropical Alexander von Humboldt (IMT AvH), Universidad Peruana Cayetano Heredia (UPCH), Lima, Perú

IIUnidad de Biotecnología Molecular, Laboratorios de Investigación y Desarrollo de Ciencia y Tecnología, Universidad Peruana Cayetano Heredia (UPCH), Lima, Perú

Correspondence

SUMMARY

High prevalence rates of human fascioliasis have been described in several regions of Peru. We surveyed 20 families in an endemic area of Peru in order to determine the proportion of infection with F. hepatica in relatives of diagnosed subjects and in order to identify associated risk factors. The study included feces and blood samples of 93 subjects. Ages ranged from one to 53 (mean = 18.6; SD = 14.2). The overall prevalence of fascioliasis by fecal examinations was 33.3% (n = 83) and by serology, 51.9% (n = 86). The prevalence in age group I (< 19 years old) by coprological and serological tests was 61.4% and 75.9%, respectively; in group II (> 19 years old) 15.4% and 37.5%. The main associated risk factor with fascioliasis was eating salads (OR = 3.29, CI = 1.2 - 9.0, p = 0.02). In conclusion, human fascioliasis is highly prevalent in the relatives of index cases and the most significant risk factor of acquiring fascioliasis in the family is eating salads in endemic areas.

Keywords: Fascioliasis; Relatives; Index case; Risk factors; Salads; Peru.

RESUMEN

Altas tasas de fasciolosis humana han sido descritas en varias regiones del Perú. Estudiamos 20 familias en una área endémica del Perú para determinar la proporción de infección con F. hepatica en los familiares de los sujetos diagnosticados y para identificar factores de riesgo asociados. El estudio incluyó un total de 93 sujetos, quienes contribuyeron con muestras de heces y sangre. Las edades comprendieron desde 1 a 53 años (media = 18.6; DS = 14.2). La prevalencia general de fasciolosis por exámenes de heces fue 33.3% (n = 83) y por serología, 51.9% (n = 86). La prevalencia en el grupo de edad I (< 19 años de edad) por pruebas coprológicas y serológicas fueron 61.4% y 75.9%, respectivamente; en el grupo II (> 19 años de edad) 15.4% y 37.5%. El principal factor de riesgo asociado con fasciolosis fue comer ensaladas (OR = 3.29, IC = 1.2-9.0, p = 0.02). En conclusión, la fasciolosis humana es altamente prevalente en familiares de los casos índices y el factor de riesgo más significante para adquirirla en la familia es comer ensaladas en las áreas endémicas.

INTRODUCTION

Fascioliasis is a parasitic disease caused by the fluke Fasciola hepatica (Linnaeus, 1758). The disease is acquired for the most part by eating watercress, other vegetables or by drinking water contaminated with metacercariae5,20. Human fascioliasis is a serious health problem in many countries, the number of reported cases have increased significantly worldwide since 19807.

There are many endemic areas throughout Peru, where the highest prevalence rates range from 8% in Cajamarca14, 28.3% in the Mantaro Valley18, and 35% in Puno by stool examinations10; and up to 36.3% by serological tests in Jauja18. These results suggest that Peru is one of the countries with the widest regional distribution of human fascioliasis brought on by F. hepatica in the world. A rural population of almost eight million people is estimated at risk in this country24. Human fascioliasis should no longer be considered as a secondary zoonosis in Peru, but as an important human parasitic disease.

Although fascioliasis occurs at all ages, the most affected are endemic-area school-children8. Most of the cases reported from hospitals or health centers are adults, probably due to the increasing complications seen in this age group, such as acute cholecystitis, biliary obstruction or cholangitis2,4,12.

One of the remarkable clinical characteristics of human fascioliasis in endemic areas is the relative absence of gastrointestinal symptoms19. Some cases are diagnosed during surgery, when adult parasites cause bile duct obstruction or hepatic dysfunction1,11,23. The percentage of patients who develop these complications is unknown. Early diagnosis and treatment might avoid expensive and risky procedures. The early detection of most human fascioliasis should be one of the priorities of Fasciola control programs.

Two case reports show a significant proportion of parasitosis in relatives of index cases with fascioliasis21. Half of the relatives of two patients with fascioliasis in Spain also were infected22, and in Germany a family of four acquired the infection, after eating raw vegetables during a visit to an endemic area; while only one of them required medical attention3.

In order to determine the infection rate or grade of F. hepatica in relation to member of index case, and in order to identify the epidemiological and clinical characteristics, we surveyed 20 families from an endemic area.

MATERIALS AND METHODS

Study area: The study was carried out in the District of Asillo, Province of Azángaro, Department of Puno, at an altitude of 3,970 meters above sea level (14º47'03" LS; 75º 21'13" LO), which occupies about 392.38 km2. Most of the homes in the district lack drinking water and electricity. The majority of the population is engaged in farming and livestock raising.

Study population: Asillo has a total population of 2,853 inhabitants who live in extreme poverty. The index case was defined as a person with F. hepatica infection diagnosed in a previous epidemiological study by our group (unpublished data). The families were all taken from index cases.

Subjects and families: A total of 20 families were included in the study; each family having between two and 13 members. We divided the ages of subjects into two groups: group I, under 19 years; and group II, over 19 years of age. The criteria of this division was to compare the high prevalence of fascioliasis found in subjects under 19 years old in other epidemiological study near this region9, and the group over 19 years that we studied.

Clinical-epidemiological interview and physical examination: The questionnaire used local colloquial language and included demographic data, sources of food and water, types of vegetables, house characteristics (including number and type of animals), defecation habits, as well as general and gastrointestinal symptoms which might be associated with F. hepatica infection.

Fecal samples: Stools were collected on two consecutive days. Coprological tests were carried out in 83 subjects. We were unable to obtain stool from 10 subjects. The samples were placed in 10% formalin on the same day that they were passed and then transported to the Parasitology Laboratory, Instituto de Medicina Tropical Alexander von Humboldt (IMTAvH) of the Universidad Peruana Cayetano Heredia for processing. Stools were examined by the Rapid Sedimentation Technique described by Lumbreras (RST)16 and the Kato-Katz Technique (KKT)13. The RST is a routinely used technique in the IMT AvH because of its low cost and high sensitivity17.

Blood sample: Five mL of venous blood were obtained from 86 consenting subjects. We were unable to obtain blood from seven subjects. Samples were centrifuged the same day, the sera were separated and transported to Lima at -4 ºC to be stored frozen at -20 ºC, until being assayed by the Fas2-ELISA as originally described6,17.

Statistical analysis: SPSS 9.0 for Windows (CopyrightÓ SPSS Inc, 1989-1999) was used. The analysis consisted of a univariate descriptive analysis that calculated frequencies and percentages for categorical variables. For continuous variables, the means and standard deviations were calculated. Bivariate analysis was applied for categorical variables with X2 or Ficher's exact test; Student's t test was used for continuous variables. A value of p < 0.05 was considered as the critical level of significance. Odds ratio (OR), confidence intervals 95% (CI) and p values were calculated. Subsequently, stepwise conditional logistic regression was used to determine independent potential risk factors that are associated to F. hepatica infection.

Ethical considerations: The study was approved by the Institutional Ethics Committee of the UPCH. Written informed consents were obtained from the parents or guardians and assent from children, respectively; all of whom were informed about the nature of the study, the benefits and the risks. All positive patients were treated with triclabendazole.

RESULTS

The general characteristics of subjects are shown in Table 1. There were no significant differences in the prevalence of fascioliasis between males and females (p > 0.05). The results of prevalence by coprological - serological tests, and univariate -multivariate analysis are shown in Tables 2 and 3, respectively.

In the clinical picture, there were two variables associated with fascioliasis, asthenia (X2 = 5.07, p = 0.024) and dizziness (X2 = 6.69, p = 0.01), among those considered as important clinical characteristics.

DISCUSSION

We found a high prevalence of human fascioliasis in the relatives of index cases. More than 50% of children in the index cases' families were infected with F. hepatica in this district, up to 75.9% in relatives under 19 years old. In a family of 11 people, nine had fascioliasis. Therefore, it is justified to rule out F. hepatica infection in all relatives of a person with this parasitosis.

In endemic areas, children are the most affected age-group, and groups under 19 years of age usually present the highest infection rates, which is consistent with our results9. There was no significant difference between males and females, which is explained by the similarity of diet in both genders, as has been observed in other studies9,10.

Univariate analysis found an association between four variables and fascioliasis: drinking water supply from canals, eating raw vegetable salads, drinking alfalfa juice and defecating in silos. Since this population has no municipal running potabilized water, it is likely that water from irrigation canals could be a significant risk factor. Viable metacercariae have been found in natural water supplies of endemic areas20.

We also found that drinking alfalfa juice was a risk factor for human fascioliasis. Drinking alfalfa juice is a common habit in areas of Peru where fascioliasis is endemic. A recent study found that drinking "emolientes" (warm vegetable beverages made mainly from alfalfa and watercress) was associated with human fascioliasis in a population of the Peruvian central Andes18. An older study found metacercariae in 10.5% of emolientes sold by vendors in a popular market in the city of Arequipa, in southern Peru15. Therefore, we can suggest that alfalfa juice may be an important risk factor, not only in this region; but also in many other areas throughout Peru as well.

Apparently, good hygienic habits decrease the risk of acquiring fascioliasis. People with good hygienic habits may be less likely to contract the infection, may having less contact with the contaminated environment than those with poor hygienic habits.

Eating raw salads was associated with fascioliasis in both univariate and logistic regression analyses. This population's diet is for the most part made up carbohydrates from tubers and legumes; while many other vegetables such as tomatoes, lettuces, onions, carrots, among others, are eaten just the same. These crops are irrigated by water canals, which may contain metacercariae. Perhaps in one or more of the edible portions of these vegetables, metacercariae are encysted, and which might explain why some cases of non-endemic areas are seen in hospitals, where fascioliasis is seldom diagnosed. Most of these patients deny having eaten watercress or having drunk alfalfa juice, but they have eaten salads.

Among the clinical symptoms, only asthenia and dizziness were statistically associated with F. hepatica infection. These are not likely to be caused by fascioliasis, which mainly produces gastrointestinal symptoms. We believe that most of the people surveyed were asymptomatic, when they were interviewed. The presence of multiple intestinal parasitic infections may have confused the clinical picture in most of the population.

We can conclude that human fascioliasis is found in a high proportion to the relatives of index cases, and this should be taken into account when fascioliasis is detected; it is usually present in most members of a family. Eating raw vegetable salads is a significant risk factor through which families may acquire the infection in endemic areas. It is recommended that patients presenting abdominal pain and low to high eosinophilia, who have recently visited an area endemic for F. hepatica, should be carefully studied so as to rule out this parasitosis and the study could be expanded to include other travel companions or family members as well.

ACKNOWLEDGMENTS

We would like to express our thanks to Víctor Neyra, Unidad de Biotecnología Molecular, Facultad de Ciencias y Filosofía Alberto Cazorla Talleri, Universidad Peruana Cayetano Heredia (UPCH) for his help in the processing of sera; Dr. Raúl Tello, Marco Canales and Carmen Quijano, Laboratorio de Parasitología, Instituto de Medicina Tropical Alexander von Humboldt, UPCH for their help in the processing of stool samples; Dr. César Wegener, Director of the Hospital Regional of Azángaro, Puno, Perú, for storing blood samples; Dr. Abdías Hurtado, President of CTAR - Puno, Perú, for providing transportation for the authors to the study area. To Dr. Humberto Guerra, M.D., Ph.D., Dr.Med., Profesor de Medicina, Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia for his assistance reviewing this paper.

  • Correspondence to
    Luis Marcos, MD
    Instituto de Medicina Tropical Alexander von Humboldt
    Universidad Peruana Cayetano Heredia
    Av. Honorio Delgado 430
    San Martín de Porras, Lima 31, Perú
    Telephone: 511-3821021. Fax: 511-3821021
    Email:
  • Received: 17 February 2005

    Accepted: 31 May 2005

    • 1. ALMENDRAS-JARAMILLO, M.; RIVERA-MEDINA, J.; SEIJAS-MOGROVEJO, J. & ALMENDRAS-JARAMILLO, K. - Hepatic fascioliasis in children: uncommon clinical manifestations. Arq. Gastroent. (S. Paulo), 34: 241-247, 1997.
    • 2. ARJONA, R.; RIANCHO, J.A.; AGUADO, J.M.; SALESA, R. & GONZALEZ-MACIAS, J. - Fascioliasis in developed countries: a review of classic and aberrant forms of the disease. Medicine (Baltimore), 74: 13-23, 1995.
    • 3. BECHTEL, U.; FEUCHT, H.E.; HELD, E.; VOGL, T. & NOTHDURFT, H.D. - Fasciola hepatica infection in a family: diagnosis and therapy. Dtsch. med. Wschr., 117: 978-982, 1992.
    • 4. BLANCAS, G.; TERASHIMA, A.; MAGUIÑA, C. et al. - Fasciolosis humana y compromiso gastrointestinal: estudio de 277 pacientes en el Hospital Nacional Cayetano Heredia, 1970-2002. Rev. Gastroent. Perú, 24:143-157, 2002.
    • 5. CHEN, M.G. & MOTT, K.E. - Progress in assessment of morbidity due to Fasciola hepatica infection: a review of recent literature. Trop. Dis. Bull., 87 (suppl.): R1-R38, 1990.
    • 6. CORDOVA, M.; REATEGUI, L. & ESPINOZA, J. - Immunodiagnosis of human fascioliasis with Fasciola hepatica cysteine proteinases. Trans. roy. Soc. trop. Med. Hyg., 93: 54-57, 1999.
    • 7. ESTEBAN, J.G.; BARGUES, M.D. & MAS-COMA, S. - Geographical distribution, diagnosis and treatment of human fascioliasis: a review. Res. Rev. Parasit., 58: 13-42, 1998.
    • 8. ESTEBAN, J.G.; FLORES, A.; ANGLES, R. et al. - A population-based coprological study of human fascioliasis in a hyperendemic area of the Bolivian Altiplano. Trop. Med. Int. Hlth., 2: 695-699, 1997.
    • 9. ESTEBAN, J.G.; FLORES, A.; ANGLES, R. & MAS-COMA, S. - High endemicity of human fascioliasis between Lake Titicaca and La Paz valley, Bolivia. Trans. roy. Soc. trop. Med. Hyg., 93: 151-156, 1999.
    • 10. ESTEBAN, J.G.; GONZALEZ, C.; BARGUES, M.D. et al. - High fascioliasis infection in children linked to a man-made irrigation zone in Perú. Trop. Med. Int. Hlth, 7: 339-348, 2002.
    • 11. HEREDIA, D.; BORDAS, J.M.; MONDELO, F. & RODES, J. - Gallbladder fascioliasis in a patient with liver cirrhosis. Med. clín. (Barcelona), 82: 768-770, 1984.
    • 12. JIMENEZ, J.; LOJA, D.; RUIZ, E. et al. - Fascioliasis hepática ¿un problema diagnóstico?. Rev. Gastroent. Perú, 21: 148-152, 2001.
    • 13. KATZ, N.; CHAVEZ, A. & PELLEGRINO, J. - A simple device for quantitative stool thick-smear technique in schistosomiasis mansoni. Rev. Inst. Med. trop. S. Paulo, 14: 397-409, 1972.
    • 14. KNOBLOCH, J.; DELGADO, E.; ÁLVAREZ, A.; REYMANN, U. & BIALEK, R. - Human fascioliasis in Cajamarca/Perú. I. Diagnostic methods and treatment with praziquantel. Trop. Med. Parasit., 36: 88-90, 1985.
    • 15. LEGUIA, P.G. - La distomatosis en el Perú. In: ZALDÍVAR, S.R. Zooparásitos de interés veterinario en el Perú Lima, Minsa, 1991. p. 3-5.
    • 16. LUMBRERAS, H.; CANTELLA, R. & BURGA, R. - Acerca de un procedimiento de sedimentación rápida para investigar huevos de Fasciola hepatica en las heces, su evaluación y uso en el campo. Rev. méd. peru., 31: 167-174, 1962.
    • 17. MACO, V.; MARCOS, L.A.; TERASHIMA, A. et al. - Fas2-ELISA y la técnica de sedimentación rápida modificada por Lumbreras en el diagnóstico de la infección por Fasciola hepatica Rev. méd. Herediana, 13: 49-57, 2002.
    • 18. MARCOS, L; MACO, V.; TERASHIMA, A. et al. - Hiperendemicidad de fasciolosis humana en el Valle del Mantaro: factores de riesgo para la infección por Fasciola hepatica Rev. Gastroent. Perú, 24: 158-164, 2004.
    • 19. MARCOS, L.A.; MACO, V.; TERASHIMA, A.; SAMALVIDES, F. & GOTUZZO, E. - Características clínicas de la infección crónica por Fasciola hepatica en niños. Rev. Gastroent. Perú, 22: 228-233, 2002.
    • 20. MAS-COMA, M.S.; ESTEBAN, J.G. & BARGUES, M.D. - Epidemiology of human fascioliasis: a review and proposed new classification. Bull. Wld. Hlth. Org., 77: 340-346, 1999.
    • 21. PETITHORY, J.C. - Familial eosinophilia: contribution of parasitology to its diagnosis. Bull. Acad. nat. Méd. (Paris), 182: 1823-1838, 1998.
    • 22. RODRIGUEZ, J.; CANUT, A.; BREZMES, M.F. et al. - Familial outbreaks of fascioliasis: usefulness of serologic investigation by enzyme immunoassay. Rev. clín. esp., 198: 33-35, 1998.
    • 23. SANCHEZ-SOSA, S.; ROJAS-ORTEGA, S.; REED-SAN ROMAN, G. & TORRES-SANTANA, M.A. - Massive hepatobiliary fascioliasis. Rev. Gastroent. Méx., 65: 179-183, 2000.
    • 24. WORLD HEALTH ORGANIZATION - Control of foodborne trematode infections. Report of a WHO Expert Committee. Wld. Hlth. Org. techn. Rep. Ser., (849), 1995.

    Correspondence to Luis Marcos, MD Instituto de Medicina Tropical Alexander von Humboldt Universidad Peruana Cayetano Heredia Av. Honorio Delgado 430 San Martín de Porras, Lima 31, Perú Telephone: 511-3821021. Fax: 511-3821021 Email: luismarcos@rcp.net.pe

    Publication Dates

    • Publication in this collection
      29 Aug 2005
    • Date of issue
      Aug 2005

    History

    • Accepted
      31 May 2005
    • Received
      17 Feb 2005
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