Flucytosine + fluconazole association in the treatment of a murine experimental model of cryptococcosis

Abstract

The efficacy of flucytosine (5-FC) and fluconazole (FLU) association in the treatment of a murine experimental model of cryptococcosis, was evaluated. Seven groups of 10 Balb C mice each, were intraperitoneally inoculated with 10(7) cells of Cryptococcus neoformans. Six groups were allocated to receive 5-FC (300 mg/kg) and FLU (16 mg/ kg), either combined and individually, by daily gavage beginning 5 days after the infection, for 2 and 4 weeks. One group received distilled water and was used as control. The evaluation of treatments was based on: survival time; macroscopic examination of brain, lungs, liver and spleen at autopsy; presence of capsulated yeasts in microscopic examination of wet preparations of these organs and cultures of brain homogenate. 5-FC and FLU, individually or combined, significantly prolonged the survival time of the treated animals with respect to the control group (p<0.01). Animals treated for 4 weeks survived significantly longer than those treated for 2 weeks (p<0.01). No significant differences between the animals treated with 5-FC and FLU combined or separately were observed in the survival time and morphological parameters. The association of 5-FC and FLU does not seem to be more effective than 5-FC or FLU alone, in the treatment of this experimental model of cryptococcosis.

Cryptococcosis; Experimental cryptococcosis; Antifungal drugs


BRIEF COMMUNICATION

Flucytosine + fluconazole association in the treatment of a murine experimental model of cryptococcosis

A. J. Bava; R. NegroniI

ICentro de Micología. Facultad de Medicina. Universidad de Buenos Aires. Paraguay 2155, Piso 11. 1121 Buenos Aires, Argentina

SUMMARY

The efficacy of flucytosine (5-FC) and fluconazole (FLU) association in the treatment of a murine experimental model of cryptococcosis, was evaluated.

Seven groups of 10 Balb C mice each, were intraperitoneally inoculated with 107 cells of Cryptococcus neoformans. Six groups were allocated to receive 5-FC (300 mg/kg) and FLU (16 mg/ kg), either combined and individually, by daily gavage beginning 5 days after the infection, for 2 and 4 weeks. One group received distilled water and was used as control.

The evaluation of treatments was based on: survival time; macroscopic examination of brain, lungs, liver and spleen at autopsy; presence of capsulated yeasts in microscopic examination of wet preparations of these organs and cultures of brain homogenate.

5-FC and FLU, individually or combined, significantly prolonged the survival time of the treated animals with respect to the control group (p<0.01). Animals treated for 4 weeks survived significantly longer than those treated for 2 weeks (p<0.01). No significant differences between the animals treated with 5-FC and FLU combined or separately were observed in the survival time and morphological parameters.

The association of 5-FC and FLU does not seem to be more effective than 5-FC or FLU alone, in the treatment of this experimental model of cryptococcosis.

Keywords: Cryptococcosis; Experimental cryptococcosis; Antifungal drugs.

Full text available only in PDF format.

Recebido para publicação em 14/06/1993.

Aceito para publicação em 13/09/1994.

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Publication Dates

  • Publication in this collection
    05 July 2006
  • Date of issue
    Dec 1994

History

  • Received
    14 June 1993
  • Accepted
    13 Sept 1994
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