PRELIMIMARY REPORT
Fulminant Labrea Hepatitis - the role of hepatitis A (HAV), B (HBV), C (HCV), and D (HDV) infection. (Preliminary report)
José Carlos Ferraz da FonsecaI,II; Luíz Carlos Lima FerreiraI,III; Leila Melo BrasilII; Marcia da Costa CastinhoII; Robson MossIV; Marilde BaroneV
IFaculdade de Ciências da Saúde - Universidade do Amazonas
IIInstituto de Medicina Tropical de Manaus - Núcleo de Virologia
IIIInstituto de Medicina Tropical de Manaus - Núcleo de Antatomopatologia
IVUnidade Hospitalar de Lábrea - Secretária de Estado da Saúde
VOspedale Civile di Busto Arsizio, Varese, Italia
Address for correspondence Address for correspondence: Prof. J. C. F. Fonseca Instituto de Medicina Tropical de Manaus - Núcleo de Virologia Av. Pedro Teixeira, 25 CEP 69.040-000, Manaus - Amazonas - Brasil
An unusual type of fulminant hepatitis, known as Labrea Hepatitis (Labrea Black Fever), has been documented in the Brazilian part of the Amazon Basin 1,2,8,9,10,12,13 Described for over 50 years10, this disease is characterized by severe hepatitis (rapid course) resulting in hepatic failure and death 2,12,13. Children, adolescent and young adults are mainly affected 1,12. The availability of histopathological liver specimens from cases of Labrea Hepatitis, showing a specific picture of microvesicular steatosis (morula-like cells) and eosinophilic necrosis, has been pointed out by Brazilian authors9. This pattern are different from those of massive and submassive necroses found in ordinary fulminant viral hepatitis. Similar clinical and histologic features of Labrea Hepatitis were found in northern Colombia5, western Venezuela16 and Central African Republic17.
Recent studies suggest that hepatitis Delta virus (HDV) infection of hepatitis B virus (HBV) carriers is responsible for the etiopathogenesis of Labrea Hepatitis2,13. Several studies have recently characterized the Amazon basin as a high endemic area for HBV and HDV infection2,14,15.
The purpose of the present study was to confirm the participation of HDV in the etiopathogenesis of Labrea Hepatitis. Serum samples from 11 patients (4 males, 7 females; aged 3-38 years; mean age 13.1 years), with post-mortem histopathological diagnosis of Labrea Hepatitis were screened for the presence of hepatitis viral markers: HAV (IgM antibodies to hepatitis A [IgM anti-HAV], Hepanostika, Organon Teknika, B. V. Holland); HBV (hepatitis B surface antigen [HBsAg], IgM antibodies to hepatitis B core antigen [IgM anti-HBc], Sorin Biomedica, Saluggia, Italy); HCV (antibodies to hepatitis C [anti-HCV], Organon Teknika, UBI HCV EIA, N. Y., USA) and HDV (Delta antigen [HDAg], IgM antibodies to hepatitis Delta [IgM anti-HD], Sorin Biomedica, Saluggia, Italy) were submitted to the enzyme immunoassay (EIA).
Serum HBV-DNA (hepatitis B virus deoxyribonucleic acid) and HDV-RNA (hepatitis D virus ribonucleic acid) were assayed using molecular hybridization technique, as previously described3,18.
On the basis of serological tests, acute Delta infection was diagnosed in four patients (36.4%). Two of this had acute HBV and HDV infection (HBsAg, IgM anti-HBc, IgM anti-HD) and the other two had chronic HBV infection (without IgM anti-HBc and HBV-DNA) with superimposed acute HDV infection (one case: HBsAg, IgM anti-HD; one case: HBsAg, HDAg, HDV-RNA). Two patients (18.2%) had acute HAV and HBV infection (IgM anti-HAV, HBsAg, IgM anti HBc). Two patients (18.2%) had acute HBV infection alone (HBsAg, IgM anti-HBc). One (9.1%) had acute HAV infection alone (IgM anti-HAV). One (9.1%) had acute HBV and HCV infection (HBsAg, IgM anti-HBc, anti-HCV). Finally, one (9.1%) patient had acute HCV infection alone (Anti-HCV). The time between the first clinical manifestations and death of 11 patients, ranged from 3 to 8 days (mean of 4.6 days).
All specimens showed a diffuse small-droplet fatty change, lytic and eosinophylic necroses with different degrees of intensity, were also seen, without evidence of massive or submassive necrosis. "Morula-like cells" were more frequently found in HDV acute superinfection, as well as in HAV/HBV acute coinfection and acute HCV infection, and less often in other forms of viral hepatitis. Lobular architecture was preserved and portal tracts revealed bile duct proliferations, mononuclear inflamatory cells infiltration.
Figure 1 shows the presence of "morula-like" cells in hepatic tissue in the fulminant form of hepatitis A (HAV), B (HBV), C (HCV) and Delta (HDV).
In this series only 4/11 (36.4%) of the patients with Labrea Hepatitis had serological evidence of acute infection by HDV. These results differ from previous studies that demonstrate a great correlation (74.0%) between the infection by HDV and Labrea Hepatitis, suggesting that the "morula-like" cells are pathognomonic of HDV superinfection in HBsAg carriers2.
Our study demonstrate that 63.6% of patients with Labrea Hepatitis had evidence of infection by HAV, HBV and HCV, either alone or simultaneously. These data differ from others authors who consider the infection by HDV the main cause of Labrea Hepatitis2. The idea that other hepatitis viruses could be involved in the etiopathogenesis of Labrea Hepatitis, HBV infection alone, lesser contribution of HAV, and Non A - Non B agents, now termed hepatitis C virus7, was suggested recently2. This hypothesis is strengthened in the present study.
The use of sophisticated and specific tests for serum markers of HBV (HBV-DNA), HCV (anti-HCV), and HDV (HDAg, HDV-RNA), permitted in this study the confirmation of the envolviment of other viral agents in the etiology of Labrea Hepatitis.
In additon, the etiopathogeny of Labrea Hepatitis could be related to genetic, immunological or age factors. This hypothesis thus remains controversial and requires further studies. On the other hand, experimental transmission studies shows antigenic variation of HDV in Bangui Hepatitis with the same clinical and histological characteristics of Labrea Hepatitis11. In this study the authors suggest a possible role for a variant HDV strain which could be responsible for severe hepatitis in Bangui. Recent data, described a new hepatitis B virus strain4 and this new HBV variant (pre-core mutation) is responsible for fulminant hepatitis in Greek patients6.
The hypothesis that variant strains of HAV, HBV, HCV and HDV could determine changes in the immune response of local patients leading to this severe form of hepatitis should be taken in consideration. However only experimental studies and the use of advanced techniques such as RNA and DNA amplification, cloning and sequencing of these viral agents could confirm this hypothesis.
ACKNOWLEDGEMENTS
The authors are deeply thankful to Prof. Mario Rizzetto, Institute of Internal Medicine, University of Turin, Italy, by the realization of part of the sorological tests by molecular hybridization and for comments and suggestions.
Recebido para publicação em 13.02.1992.
Aceito para publicação em 13.08.1992.
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Publication Dates
-
Publication in this collection
11 Sept 2006 -
Date of issue
Dec 1992
History
-
Received
13 Feb 1992 -
Accepted
13 Aug 1992