: acquired resistance in mice by implantation of young irradiated worms into the portal system

Coelho, Paulo Marcos Z.; Mello, Rómulo Teixeira de; Tavares, Carlos Alberto P.

doi:10.1590/S0036-46651989000100003

Abstract

In two distinct experiments, immature S. mansoni worms (LE strain, Belo Horizonte, Brazil), aged 20 days, obtained from the portal system of white outbred mice, were irradiated with 14 and 4 Krad, respectively. Afterwards, the worms were directly inoculated into the portal vein of normal mice. Inoculation was performed with 20 irradiated worms per animal. Fifty days after inoculation, the mice that received 4 and 14 Krad-irradiated worms and their respective controls were infected with S. mansoni cercariae (LE strain), by transcutaneous route. Twenty days after this challenge infection, the animals were sacrificed and perfused for mature irradiated (90-day-old) and immature (20-day-old) worm counts. Analysis of the results showed that statistically significant protection against cercariae occurred in both groups with irradiated worms.

Schistosoma mansoni; - irradiated worms; immunoprotection; transplantation of worms

ORIGINAL ARTICLES

Schistosoma mansoni: acquired resistance in mice by implantation of young irradiated worms into the portal system(¹ (1 ) This work was supported, in part, by CNPq and PINEP, Brazil. )

Schistosoma mansoni: resistência adquirida em camundongos pela implantação de vermes jovens irradiados no sistema porta

Paulo Marcos Z. Coelho^I; Rómulo Teixeira de Mello^II; Carlos Alberto P. Tavares^III

^IDepartamento de Parasitologia e Grupo Interdepartamental de Estudos sobre Esquistossomose (GIDE), ICB/UFMG. Caixa Postal 2486. CEP 30161 Belo Horizonte, MG, Brasil

^IIDepartamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia da UFMG e Grupo Interdepartamental de Estudos sobre Esquistossomose (GIDE), ICB/UFMG, Belo Horizonte, MG, Brasil

^IIIDepartamento de Bioquimica-Imunologia, ICB/UFMG, Belo Horizonte, MG, Brasil

SUMMARY

In two distinct experiments, immature S. mansoni worms (LE strain, Belo Horizonte, Brazil), aged 20 days, obtained from the portal system of white outbred mice, were irradiated with 14 and 4 Krad, respectively. Afterwards, the worms were directly inoculated into the portal vein of normal mice. Inoculation was performed with 20 irradiated worms per animal. Fifty days after inoculation, the mice that received 4 and 14 Krad-irradiated worms and their respective controls were infected with S. mansoni cercariae (LE strain), by transcutaneous route. Twenty days after this challenge infection, the animals were sacrificed and perfused for mature irradiated (90-day-old) and immature (20-day-old) worm counts. Analysis of the results showed that statistically significant protection against cercariae occurred in both groups with irradiated worms.

Key words:Schistosoma mansoni - irradiated worms - immunoprotection - transplantation of worms.

RESUMO

Em dois experimentos distintos, vermes imaturos de Schistosoma mansoni, com 20 dias de idade, obtidos do sistema porta de camundongos albinos, foram irradiados com 14 e 4 Krad e posteriormente inoculados diretamente na veia porta de camundongos normais. Cada animal, de cada experimento específico, recebeu 20 vermes irradiados. Decorridos 50 dias de inoculação, os camundongos com os vermes irradiados com 4 e 14 Krad e seus respectivos controles foram infectados pela via transcutânea, com cercarias da cepa LE de Schistosoma mansoni. No 20º dia após esta infecção desafio, os camundongos foram sacrificados e perfundidos para as contagens dos vermes maduros irradiados (90 dias de idade) e imaturos (20 dias de idade).

A análise dos resultados mostrou que ocorreu proteção, estatisticamente significativa, contra cercarias nos grupos previamente inoculados com vermes irradiados com as doses de 4 e 14 Krad.

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ACKNOWLEDGEMENTS

The Authors gratefully acknowledge Mrs. Vera de Paula Ribeiro for translating the manuscript, and the technical assistance of Mr. Alberto Geraldo dos Santos, Mrs. Alice Neni Faria, Miss Zenir de Souza, Mr, José de Souza Filho, Mr. Adelino Ferreira and Mr. Maurício V. da Costa.

Recebido para publicação em 06/6/1988.

1. BARBOSA, M. A.; PELLEGRINO, J.; COELHO, P. M. Z. & SAMPAIO, I. B. M. - Quantitative aspects of the migration and evolutive asynchronism of Schistosoma mansoni in mice. Rev. Inst. Med. trop. S. Paulo, 20: 121-132, 1978.
2. COELHO, P. M. Z.; RASO, P.; MELLO, R. T.; HAR-TRUNG, N.; TAVARES, C. A. P. & ANTUNES, C. M. F. - Schistosoma mansoni: diferenças entre cepas no hospedeiro vertebrado. In: INTERNATIONAL SYMPOSIUM ON SCHISTOSOMIASIS, 1., Rio de Janeiro, Brazil, 1987. Abstracts Abst. Nş 132.
3. DEAN, D. A. - Schistosoma and related genera: acquired resistance in mice. A review. Exp. Parasit., 55: 1-104,1983.
4. DEAN, D. A. - Sites of schistosome elimination. In: INTERNATIONAL SYMPOSIUM ON SCHISTOSOMIASIS, 1.; Rio de Janeiro, Brazil, 1987. Abstracts Abst. N: 134.
5. DEAN, D. A.; CIOLI. D. & BUKOWSKI, M. A. - Resistance induced by normal and irradiated Schistosoma mansoni: ability of various worm stages to serve as inducers and targets in mice. Amer. J. trop. Med. Hyg., 30: 1026-1032, 1981.
6. FORD, M. J.; BICKLE, Q. D. & TAYLOR, M. G. - Immunization of rats against Schistosoma mansoni using irradiated cercariae, lung schistosomula and liver-stage worms. Parasitology, 89: 327-344, 1984.
7. PELLEGRINO, J. & SIQUEIRA, A. F. - Técnica de perfusão para colheita de Schistosoma mansoni em cobaias experimentalmente infestadas. Rev. bras. Malar., 8: 589-597, 1956.
8. PHILLIPS, S. M. & COLLEY, D. G. - Immunologic aspects of host responses to schistosomiasis: resistance, immuhopathology, and eosinophil involvement. Progr. Allergy, 24: 49-182, 1978.
9. PHILLIPS, S. M.; REID, W. A. & SADUN, E. H. - The cellular and humoral immune response to Schistosoma mansoni infection in inbred rats (II). Mechanisms during re-exposure. Cell. Immunol., 28: 75-89, 1977.
10. ROCHA, M. O. & COELHO, P. M. Z. - The importance of skin and pulmonary phases to the development of Schistosoma mansoni in albino mice. Rev. Inst. Med. trop. S. Paulo, 22: 157-163, 1980.
11. SMITHERS, S. R. & DOENHOFF, M. - Schistosomiasis. In: COHEN, S. & WARREN, K. S. - Immunity to parasitic infection. London, Blackwell, 1982.
12. SMITHERS, S. R. & TERRY, R. J. - Immunity in Schistosomiasis. Ann. N. Y. Acad. Sci., 160: 826-840, 1969.
13. W. H. O. - Immunity in Schistosomiasis. Bull. Wld. Hlth. Org., 51: 553-595, 1974.

(1

) This work was supported, in part, by CNPq and PINEP, Brazil.

Publication Dates

Publication in this collection
18 Feb 2011
Date of issue
Feb 1989

History

Received
06 June 1988

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BARBOSA, M. A.; PELLEGRINO, J.; COELHO, P. M. Z. & SAMPAIO, I. B. M. - Quantitative aspects of the migration and evolutive asynchronism of Schistosoma mansoni in mice. Rev. Inst. Med. trop. S. Paulo, 20: 121-132, 1978.

[2] 2. COELHO, P. M. Z.; RASO, P.; MELLO, R. T.; HAR-TRUNG, N.; TAVARES, C. A. P. & ANTUNES, C. M. F. - Schistosoma mansoni: diferenças entre cepas no hospedeiro vertebrado. In: INTERNATIONAL SYMPOSIUM ON SCHISTOSOMIASIS, 1., Rio de Janeiro, Brazil, 1987. Abstracts Abst. Nş 132.

[3] 3. DEAN, D. A. - Schistosoma and related genera: acquired resistance in mice. A review. Exp. Parasit., 55: 1-104,1983.

[4] 4. DEAN, D. A. - Sites of schistosome elimination. In: INTERNATIONAL SYMPOSIUM ON SCHISTOSOMIASIS, 1.; Rio de Janeiro, Brazil, 1987. Abstracts Abst. N: 134.

[5] 5. DEAN, D. A.; CIOLI. D. & BUKOWSKI, M. A. - Resistance induced by normal and irradiated Schistosoma mansoni: ability of various worm stages to serve as inducers and targets in mice. Amer. J. trop. Med. Hyg., 30: 1026-1032, 1981.

[6] 6. FORD, M. J.; BICKLE, Q. D. & TAYLOR, M. G. - Immunization of rats against Schistosoma mansoni using irradiated cercariae, lung schistosomula and liver-stage worms. Parasitology, 89: 327-344, 1984.

[7] 7. PELLEGRINO, J. & SIQUEIRA, A. F. - Técnica de perfusão para colheita de Schistosoma mansoni em cobaias experimentalmente infestadas. Rev. bras. Malar., 8: 589-597, 1956.

[8] 8. PHILLIPS, S. M. & COLLEY, D. G. - Immunologic aspects of host responses to schistosomiasis: resistance, immuhopathology, and eosinophil involvement. Progr. Allergy, 24: 49-182, 1978.

[9] 9. PHILLIPS, S. M.; REID, W. A. & SADUN, E. H. - The cellular and humoral immune response to Schistosoma mansoni infection in inbred rats (II). Mechanisms during re-exposure. Cell. Immunol., 28: 75-89, 1977.

[10] 10. ROCHA, M. O. & COELHO, P. M. Z. - The importance of skin and pulmonary phases to the development of Schistosoma mansoni in albino mice. Rev. Inst. Med. trop. S. Paulo, 22: 157-163, 1980.

[11] 11. SMITHERS, S. R. & DOENHOFF, M. - Schistosomiasis. In: COHEN, S. & WARREN, K. S. - Immunity to parasitic infection. London, Blackwell, 1982.

[12] 12. SMITHERS, S. R. & TERRY, R. J. - Immunity in Schistosomiasis. Ann. N. Y. Acad. Sci., 160: 826-840, 1969.

[13] 13. W. H. O. - Immunity in Schistosomiasis. Bull. Wld. Hlth. Org., 51: 553-595, 1974.

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: acquired resistance in mice by implantation of young irradiated worms into the portal system

Schistosoma mansoni: resistência adquirida em camundongos pela implantação de vermes jovens irradiados no sistema porta

Abstract

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History