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Anatomy of the Mimosa pilulifera (Leguminosae) seedling growing in petroleum contaminated and bioremediated soil

ABSTRACT

The demand of oil and its derivatives have provoked accidents, contaminating areas of the planet. The majority of are relative to the effect in seed germination and plant development. Little is known on the effect of the hydrocarbons in the structure of the tissues and plant cells. This work intends to analyse the effect of the ground contaminated by oil and the bioremediated ground on the anatomy of seedlings of Mimosa pilulifera. The experiment was carried through with ground proceeding of the REPAR/PETROBRÁS, Araucária-PR, where a leak of oil occurred in 2000. The experiment consisted of three treatments: petroleum contaminated soil, bioremediated soil and non contaminated soil, with five repetitions. Thirty days after sowing, 10 seedlings were collected per treatment. The root, the hipocotyl, the cotyledon and the eophyll were analysed anatomically and were submitted to histochemical tests with Sudan III, lugol and ferric chloride. The diameter of the vascular cylinder and the thickness of the cortex of the root, the diameter of the hipocotyl and the thickness of the cotyledons and eophylls were measured. The cellular structure of the root of M. pilulifera suffered greater interference in the contaminated soil that the biorremediated. In shoots, the only change was the reduction in the thickness of eophyll in contaminated soil.

Key words:
root; hipocotyl; cotyledon; eophyll; hydrocarbons

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