Kaviani, Behzad; Almasi, Ava; Sedaghathoor, Shahram; Khoddamzadeh, Amir Ali

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Sumário

Original Paper • Rodriguésia 72 • 2021 • https://doi.org/10.1590/2175-7860202172053 copiar

A protocol for fast in vitro propagation of Impatiens hawkeri cv. Sweeties Blue Star, an important ornamental-cover species

Autoria SCIMAGO INSTITUTIONS RANKINGS

Resumo

Impatiens hawkeri está entre as três principais plantas para forragem do mundo. Não existe um protocolo eficiente para a micropropagação rápida de Impatiens hawkeri cv. Sweeties Blue Star. Nódulos individuais germinados em meio Murashige e Skoog (MS) suplementado com ácido 1-naftalenoacético (NAA; 0,00, 0,10 e 0,50 mg l- 1), 6-benziladenina (BA; 0,00, 0,10, 0,50 e 1,00 mg l - 1) e cinetina (KIN; 0,00, 0,10, 0,50 e 1,00mg l-1). O comprimento dos brotos foi maior no meio contendo 0,10 mg l-1 de ANA e controle. O maior número de brotações (14,06 e 14,00 por explante) ocorreu em meio suplementado com 1,00 mg l-1 BA, 0,50 mg l-1 NAA e 1,00 mg l-1 BA, 0,10 mg l-1 NAA, respectivamente. O 0,10 mg l-1 de ANA em conjunto com 1,00 mg l-1 de BA foi considerado superior para a produção de folhas (57,13). O comprimento máximo da raiz (33,80 mm) e o número de raízes (29,13) foram obtidos em meio suplementado com 0,10 mg l-1 de ANA sem BA e KIN. As mudas foram transplantadas para vasos com perlita e turfa (na proporção de 1: 1) e transferidas para casa de vegetação para aclimatação. As mudas regeneradas eram morfologicamente idênticas às plantas-mãe. Na transferência ex vitro, 100% das plantas sobreviveram.

Palavras-chave:
Balsaminaceae; micropropagação; organogênese; ornamentais; reguladores de crescimento vegetal

Source of variations	df	Mean comparison
Source of variations	df	Shoot height	Shoot number	Leaf number	Leaf fresh weight	Root length	Root number	Root fresh weight
NAA	2	0.0007^ = Significant at the 0.01 probability level	89.99^ = Significant at the 0.01 probability level	1199.34^ = Significant at the 0.01 probability level	2.35^ = Significant at the 0.01 probability level	0.045^ = Significant at the 0.01 probability level	0.0023^ = Significant at the 0.01 probability level	0.094^ = Significant at the 0.01 probability level
CKs	6	0.0113^ = Significant at the 0.01 probability level	104.48^ = Significant at the 0.01 probability level	1528.12^ = Significant at the 0.01 probability level	2.82^ = Significant at the 0.01 probability level	0.221^ = Significant at the 0.01 probability level	0.0004^ = Significant at the 0.01 probability level	0.024^ = Significant at the 0.01 probability level
NAA × CKs	12	0.0019^ = Significant at the 0.01 probability level	13.48^ = Significant at the 0.01 probability level	173.17^ = Significant at the 0.01 probability level	0.34^ = Significant at the 0.01 probability level	0.039^ = Significant at the 0.01 probability level	0.0006^ = Significant at the 0.01 probability level	0.026^ = Significant at the 0.01 probability level
Error	42	0.0001	0.41	2.13	0.01	0.001	0.0001	0.002
CV (%)	-	3.52	8.31	4.85	9.50	2.58	3.39	2.81

PGRs (mg l^–1) NAA	BA	KIN	Mean comparison
PGRs (mg l^–1) NAA	BA	KIN	Shoot height (mm)	Shoot number	Leaf number	Leaf fresh weight (g)	Root length (mm)	Root number	Root fresh weight
0.0	0.0	0.0	25.13±0.97^a	2.93 ±0.97^h	10.66 ±3.34^k	0.58±0.13^h	25.06 ±1.60^d	15.33 ±0.77^h	0.41 ± 0.020^hi
	0.1	0.0	14.93 ±1.07^d	5.00 ±0.90^fg	20.06 ± 3.74^hi	0.90 ±0.15^fg	19.13 ± 1.77^fg	19.00 ±0.80^fg	0.43 ± 0.021^fghi
	0.5	0.0	12.00 ±1.00^ghi	5.13±0.87^f	20.93 ± 3.27^hi	0.96 ± 0.15^ef	13.00 ±1.55^ijkl	19.86 ±0.84^fg	0.51±0.023^cd
	1.0	0.0	9.33 ± 1.06^k	8.06 ± 0.74^d	31.00 ±3.20^f	1.33±0.16^d	8.00 ±1.60^p	21.80 ±0.70^de	0.47±0.023^def
	0.0	0.1	13.33 ± 0.92^efg	3.93 ±0.88^gh	15.20 ±3.20^j	0.69 ±0.13^gh	18.00 ±1.47^gh	21.53 ± 0.67^e	0.48 ± 0.023^de
	0.0	0.5	12.60 ±1.10^ghi	5.06 ±0.84^fg	21.00 ±3.30^hi	0.88 ±0.14^fg	12.80 ±1.47^ijkl	19.00 ±0.80^fg	0.43 ± 0.021^efgh
	0.0	1.0	11.06 ±0.60^hij	7.00 ± 0.90^e	29.13 ±3.37^f	1.28±0.16^d	9.90 ±1.47^no	17.60 ± 0.70^gh	0.38 ± 0.020^ij
0.1	0.0	0.0	24.18 ± 1.52^a	2.86 ±0.77^h	11.86 ±3.14^k	0.54 ±0.11^h	33.80 ± 1.66^a	29.13 ± 0.77^a	0.65 ± 0.026^a
	0.1	0.0	14.13 ±1.37^de	6.33 ± 0.84^e	25.00 ±3.20^g	1.15±0.15^de	19.80 ±1.50^ef	27.26 ± 0.74^b	0.57 ±0.026^b
	0.5	0.0	9.26±1.14^k	12.06 ±0.80^ab	48.40 ± 3.50^c	2.31 ± 0.14^a	11.46 ±1.64^klmn	22.80 ± 0.70^cde	0.48 ± 0.023^def
	1.0	0.0	16.60 ± 0.98^e	14.00 ± 0.91^a	57.13 ± 3.07^a	2.37±0.14^a	31.00 ±1.64^b	19.40 ±0.67^fg	0.41 ±0.020^ghi
	0.0	0.1	13.66 ±0.94^def	5.06 ±0.84^fg	21.13 ±3.16^hi	0.88 ±0.14^fg	16.66 ±1.44^h	23.60 ± 0.66^cd	0.51±0.023^cd
	0.0	0.5	12.40 ±1.10^fgh	10.13 ± 0.87^c	39.33 ± 2.87^e	1.73±0.13^c	14.33 ±1.67ⁱ	21.60 ± 0.60^e	0.54 ± 0.023^bc
	0.0	1.0	9.93±1.04^jk	12.93 ±0.94^ab	43.53 ±3.47^d	2.05 ±0.11^b	11.26 ±1.64^lmn	19.66 ±0.64^f	0.41 ±0.020^ghi
0.5	0.0	0.0	21.53 ±0.98^b	2.86 ± 0.74^h	11.20 ±3.10^k	0.48 ±0.15^h	29.20 ± 1.70^c	26.66 ± 0.74^c	0.47±0.023^def
	0.1	0.0	14.80 ±1.00^de	5.00 ±0.82^fg	21.66 ±3.05^h	0.94 ± 0.15^ef	21.26 ±1.62^de	19.46 ±0.74^fg	0.46 ± 0.022^defg
	0.5	0.0	12.20 ± 0.90^fghi	6.73 ± 0.87^e	29.80 ±3.30^f	1.35±0.16^d	13.26 ±1.59^ij	18.96 ±0.84^fg	0.36 ±0.019^j
	1.0	0.0	10.73 ±1.17^ijk	14.06 ± 0.90^a	55.00 ± 3.20^ab	2.36 ± 0.14^a	12.20 ±1.60^jklm	19.33 ±0.70^fg	0.43±0.210^efghi
	0.0	0.1	9.73 ±1.07^jk	5.26 ± 0.94^f	18.46±3.44ⁱ	0.82 ±0.11^fg	10.16 ±1.61^no	18.33 ±0.67^fg	0.38 ± 0.020^hij
	0.0	0.5	10.13 ±0.95^jk	12.20 ±0.80^b	43.13 ±3.40^d	1.91 ±0.16^bc	10.73 ±1.60^mno	19.13 ±0.66^fg	0.46 ± 0.022^defg
	0.0	1.0	11.80 ±1.00^ghi	13.73 ± 0.87^a	53.60 ±3.11^b	2.30 ± 0.15^a	13.13 ±1.48^ijk	19.33 ±0.70^fg	0.41 ±0.020^ghi

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[1] 6 Author for correspondence: b.kaviani@yahoo.com kaviani@iaurasht.ac.ir