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Organização estrutural e ultra-estrutural das células vegetativas e da estrutura plurilocular de Hincksia mitchelliae (Harvey) P. C. Silva (Ectocarpales, Phaeophyceae)

Structural and ultrastructural organization of the vegetative cells and plurilocular structure of Hincksia mitchlelliae (Harvey) P. C. Silva (Ectocarpales, Phaeophyceae)

RESUMO

O presente estudo tem por objetivo contribuir para o estudo de caracteres sub-celulares que poderão ser utilizados na taxonomia das Phaeophyceae filamentosas, fornecendo dados sobre a estrutura e ultra-estrutura das células vegetativas e do estágio reprodutivo plurilocular de H. mitchelliae. Para tanto, estudos de microscopia de luz e eletrônica de transmissão foram realizados. As células vegetativas e reprodutivas de H. mitchelliae são uninucleadas, revestidas por uma parede celulósica, outros polissacarídeos e proteínas. A presença de um núcleo por célula, a organização dos tilacóides nos cloroplastos, formando bandas com três tilacóides, dispostos longitudinalmente ao eixo maior da organela e ausência de tilacóide no pirenóide foram similares a outras Phaeophyceae. H. mitchelliae também exibiu características ultra-estruturais que estão geralmente associadas a outras espécies, de ordens menos avançadas de Phaeophyceae como a presença de um proeminente pirenóide, dictiossomos perinucleares e plasmodesmos. A morfologia e a organização dos cloroplastos, bem como a presença ou ausência de pirenóide são características importantes dentro das Phaeophyceae. A formação de um novo pirenóide, em estágio de diferenciação, foi observada nos cloroplastos das células vegetativas. Ambas as células apresentaram núcleo com um grande nucléolo, sugerindo uma alta atividade metabólica. Muitos corpos osmiofílicos, os fisóides, foram vistos no citoplasma das células vegetativas e reprodutivas. As células da estrutura plurilocular madura de H. mitchelliae diferiram das células vegetativas por apresentar tamanho reduzido, citoplasma denso e desprovidas de vacúolos.

Palavras-chave:
Hincksia mitchelliae; organização estrutural e ultra-estrutural

ABSTRACT

This study aims to contribute to the suty of sub-cellular characters that may be used in the taxonomy of filamentous Phaeophyceae. Studies of light and electron microscopy transmission provided data on the structure and ultrastructure of the cells in the vegetative stage and plurilocular reproductive structures of H. mitchelliae. Vegetative and reproductive cells of H. mitchelliae are uni-nucleated, coated with a cellulose wall, other polysaccharides and proteins. The presence of a single nucleus, organization of thylakoids in the chloroplasts forming three thylakoids per band, longitudinally arranged at the major axis of the organelles, and absence of thylakoids in the pyrenoid are similar to those of other Phaeophyceae. H mitchelliae also exhibits ultrastructural characteristics that are generally associated with other species from less advanced orders of Phaeophyceae: the presence of a prominent pyrenoid, perinuclear dictyosomes and plasmodesmatas. The morphology and the organization of chloroplasts as well the presence or absence of pyrenoid are important features in the Phaeophyceae. The formation of a new pyrenoid during differentiation periods was observed in chloroplasts of vegetative cells. Both cells presented a nucleus with a large nucleolus, suggesting an intense metabolic activity. Many osmiophilic bodies called physodes were observed in the cytoplasm of the vegetative and reproductive cells. Cells of matured plurilocular structure of H. mitchelliae differed from vegetative cells due to its reduced size, dense cytoplasm, and absence of vacuoles.

Key words:
Hincksia mitchelliae; structural organization and ultrastructure

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Datas de Publicação

  • Publicação nesta coleção
    Oct-Dec 2008

Histórico

  • Recebido
    Out 2007
  • Aceito
    Jun 2008
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