Gonçalves, Fernanda Manhães Braga; Ramos, Amaro Chaves; Mathias, Marcelo da Silva; Sales, Queitilane de Souza; Ramos, Carolina Chaves; Antunes, Fernanda; Oliveira, Rodrigo Rodrigues de

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Sumário

Pharmacognosy • Rodriguésia 71 • 2020 • https://doi.org/10.1590/2175-7860202071048 copiar

Phytochemical analysis and hypotensive activity of Ipomoea pes-caprae on blood pressure of normotensive rats

Autoria SCIMAGO INSTITUTIONS RANKINGS

Resumo

Ipomoea pes-caprae (Convolvulaceae), conhecida popularmente como “salsa-da-praia”, é usada na medicina popular para o tratamento de várias doenças, incluindo a hipertensão. O objetivo desta pesquisa foi avaliar a atividade hipotensora atribuída a essa espécie e identificar os compostos bioativos responsáveis por esse efeito. O extrato hidroalcoólico das partes aéreas de Ipomoea pes-caprae (PIEA) foi fracionado por cromatografia em coluna com Amberlite XAD-2, obtendo-se uma fração metanólica (PIEA-M). A investigação do perfil químico de PIEA-M foi realizada por Cromatografia :íquida de Ultra-Eficiência acoplada a Espectrometria de Massas (CLUE-EM) e isolamento de compostos por Cromatografia Contracorrente de Alto Desempenho (CCCAD). Avaliações hemodinâmicas dos PIEA, PIEA-M e compostos isolados foram realizadas em ratos normotensos. A atividade locomotora de PIEA e PIEA-M foi realizada pelo teste de Rota Rod usando camundongos suíços. A análise CLUE-EM do PIEA-M levou à identificação de 11 substâncias correspondentes a sete ácidos fenólicos e quatro flavonoides. Por meio de CCCAD, a quercetina 6”-O-acetil-3-O-glicosídeo (1), uma mistura de quercetina-3-O-galactosídeo e quercetina-3-O-glicosídeo (2) e quercetina 3-O-glicosídeo (3) foram isolados. PIEA, PIEA-M e as substâncias obtidas (1 e 2) reduziram significativamente a pressão sanguínea. Além disso, o PIEA e o PIEA-M causaram um prejuízo significativo na atividade locomotora dos camundongos. Estes resultados indicam o potencial de Ipomoea pes-caprae como fonte de agentes hipotensores, sugerindo que os compostos fenólicos presentes na espécie são responsáveis por sua atividade hipotensora.

Palavras-chave:
Convolvulaceae; hipertensão; Ipomoea pes-caprae; compostos fenólicos; rota-rod

Peak	RT (min)	λ_máx (nm)	[M - H]^-	Theoretical mass (m/z)	Error (ppm)	Molecular formula	MS/MS (% abundance)	Compound	References
1	7.5	216; 324	353.0897	353.0878	5.38	C₁₆H₁₈O₉	191 (100), 179 (36), 135 (65)	3-O-Caffeoylquinic acid	*Jaiswal et al.* 2014**Jaiswal R, Müller H, Müller A, Karar MGE & Kuhnert N (2014) Identification and characterization of chlorogenic acids, chlorogenic acid glycosides and flavonoids from Lonicera henryi L. (Caprifoliaceae) leaves by LC-MSn. Phytochemistry 30: 1-12.
2	11.9	217; 322	179.0335	179.0349	-7.82	C₉H₈O₄	135 (100)	Caffeic acid	*Santos et al.* 2014**Santos J, Oliveira MBPP, Ibáñez E & Herrero M (2014) Phenolic profile evolution of different ready-to-eat baby-leaf vegetables during storage. Journal of Chromatography A 1327: 118-131.
3	13.7	217; 324	353.0899	353.0878	5.95	C₁₆H₁₈O₉	191 (100)	5-O-Caffeoylquinic acid	*Jaiswal et al.* 2014**Jaiswal R, Müller H, Müller A, Karar MGE & Kuhnert N (2014) Identification and characterization of chlorogenic acids, chlorogenic acid glycosides and flavonoids from Lonicera henryi L. (Caprifoliaceae) leaves by LC-MSn. Phytochemistry 30: 1-12.
4	15.6	217; 325	353.0898	353.0878	5.66	C₁₆H₁₈O₉	191 (100), 135 (76), 173 (61), 179 (57)	4-O-Caffeoylquinic acid	*Ncube et al.* 2014**Ncube EN, Mhlongo MI, Piater LA, Steenkamp PA, Dubery IA & Madala NE (2014) Analyses of chlorogenic acids and related cinnamic acid derivatives from Nicotiana tabacum tissues with the aid of UPLC-QTOF-MS/MS based on the in-source collision-induced dissociation method. Chemistry Central Journal 8: 66.
5	37.7	254; 351	463.0881	463.0882	-0.22	C₂₁H₂₀O₁₂	300 (100)	quercetin 3-O-galactoside	*Santos et al.* 2014Santos J, Oliveira MBPP, Ibáñez E & Herrero M (2014) Phenolic profile evolution of different ready-to-eat baby-leaf vegetables during storage. Journal of Chromatography A 1327: 118-131.; Zhao et al. 2017**Zhao Y, Wu X, Yu L & Chen P (2017) Retention of polyphenols in blueberries (Vaccinium corymbosum) after different cooking methods, using UHPLC-DAD-MS based metabolomics. Journal of Food Composition and Analysis 56: 55-66.
6	41.5	254; 359	463.0887	463.0882	1.08	C₂₁H₂₀O₁₂	300 (100)	quercetin 3-O-glucoside	*Santos et al.* 2014Santos J, Oliveira MBPP, Ibáñez E & Herrero M (2014) Phenolic profile evolution of different ready-to-eat baby-leaf vegetables during storage. Journal of Chromatography A 1327: 118-131.; Zhao et al. 2017**Zhao Y, Wu X, Yu L & Chen P (2017) Retention of polyphenols in blueberries (Vaccinium corymbosum) after different cooking methods, using UHPLC-DAD-MS based metabolomics. Journal of Food Composition and Analysis 56: 55-66.
7	59.8	212; 324	515.1202	515.1195	1.36	C₂₅H₂₄O₁₂	179 (100), 173 (95), 353 (50), 191 (48), 335 (25), 161 (20), 135 (13)	3,4-di-O-caffeoylquinic acid	*Heyman et al.* 2015**Heyman HM, Senejoux F, Seibert I, Klimkait T, Maharaj VJ & Meyer JJM (2015) Identification of anti-HIV active dicaffeoylquinic- and tricaffeoylquinic acids in Helichrysum populifolium by NMR-based metabolomic guied fractionation. Fitoterapia 103: 155-164.
8	63.2	214; 326	515.1201	515.1195	1.16	C₂₅H₂₄O₁₂	191 (100), 179 (72), 353 (29), 173 (5)	3,5-O-Dicaffeoylquinic acid	*Clifford et al.* 2005**Clifford MN, Knight S & Kuhnert N (2005) Discriminating between the six isomers of dicaffeoylquinic acid by LC-MSn. Journal of Agricultural and Food Chemistry 53: 3821-3832..
9	68.9	254; 347	505.0992	505.0988	0.79	C₂₃H₂₂O₁₃	300 (100)	quercetin 3-O-acetylgalactoside	*An et al.* 2013An H, Wang H, Lan Y, Hashi Y & Chen S (2013) Simultaneous qualitative and quantitative analysis of phenolic acids and flavonoids for the quality control of Apocynum venetum L. leaves by HPLC-DAD-ESI-IT-TOF-MS and HPLC-DAD. Journal of Pharmaceutical and Biomedical Analysis 85: 295-304.; Zhao et al. 2017**Zhao Y, Wu X, Yu L & Chen P (2017) Retention of polyphenols in blueberries (Vaccinium corymbosum) after different cooking methods, using UHPLC-DAD-MS based metabolomics. Journal of Food Composition and Analysis 56: 55-66..
10	70.8	254; 349	505.0987	505.0988	-0.20	C₂₃H₂₂O₁₃	300 (100)	quercetin 3-O-acetylglucoside	*An et al.* 2013An H, Wang H, Lan Y, Hashi Y & Chen S (2013) Simultaneous qualitative and quantitative analysis of phenolic acids and flavonoids for the quality control of Apocynum venetum L. leaves by HPLC-DAD-ESI-IT-TOF-MS and HPLC-DAD. Journal of Pharmaceutical and Biomedical Analysis 85: 295-304.; Zhao et al. 2017**Zhao Y, Wu X, Yu L & Chen P (2017) Retention of polyphenols in blueberries (Vaccinium corymbosum) after different cooking methods, using UHPLC-DAD-MS based metabolomics. Journal of Food Composition and Analysis 56: 55-66..
11	90.6	212; 326	515.1194	515.1195	-0.19	C₂₅H₂₄O₁₂	173 (100), 179 (93), 353 (65), 191 (33), 135 (11)	4,5-O-Dicaffeoylquinic acid	*Clifford et al.* 2005**Clifford MN, Knight S & Kuhnert N (2005) Discriminating between the six isomers of dicaffeoylquinic acid by LC-MSn. Journal of Agricultural and Food Chemistry 53: 3821-3832..

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E-mail: rodriguesia@jbrj.gov.br

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[1] 4Author for correspondence: fernandinha.mbraga@gmail.com