Ramos, Jéssica Celeste Mônico; Ribeiro, Luan Marlon; Nunes, Geisianny Pereira; Soares, Jackeline Schultz; Sorgato, José Carlos

Jéssica Celeste Mônico Ramos ⁷7Author for correspondence: jessica_monico13@hotmail.com

Federal University of Grande Dourados - UFGD, Faculty of Agrarian Sciences, Dourados, MS, Brazil

http://orcid.org/0000-0002-3987-6726

Luan Marlon Ribeiro

Federal University of Grande Dourados - UFGD, Faculty of Agrarian Sciences, Dourados, MS, Brazil

http://orcid.org/0000-0002-7278-7696

Geisianny Pereira Nunes

Federal University of Grande Dourados - UFGD, Faculty of Agrarian Sciences, Dourados, MS, Brazil

http://orcid.org/0009-0006-2248-7632

Jackeline Schultz Soares

Federal University of Grande Dourados - UFGD, Faculty of Agrarian Sciences, Dourados, MS, Brazil

http://orcid.org/0000-0001-9497-2975

José Carlos Sorgato

Federal University of Grande Dourados - UFGD, Faculty of Agrarian Sciences, Dourados, MS, Brazil

http://orcid.org/0000-0003-0001-5467

7Author for correspondence: jessica_monico13@hotmail.com

Area Editor: Dr. Nelson Santos Junior

Figuras (4)
Tabelas (2)

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Figure 1
Spectral energy distribution of LEDs and of the fluorescent lamp.

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Figure 2
a-b. Number of leaves (NL) and number of roots (NR) of Schomburgkia crispa in function after four months of in vitro cultivation - a. different light sources; b. sealing system. FL1-100% white LED lamp; FL2-100% blue LED lamp; FL3-100% red LED lamp; FL4-50% white + 25% red + 25% blue LED lamps; FL5-50% red + 50% blue LED lamps; FL6-25% red + 75% blue LED lamps; FL7-75% red + 25% blue LED lamps; FL8- white fluorescent lamp. CSS = conventional sealing system; SSGE = sealing system with gas exchange. Same letters differ by Scott-Knott test (p < 0.05) (a) and F test (p < 0.05) (b).

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Figure 3
Schomburgkia crispa plants at 120 days of in vitro cultivation as a function of light sources and sealing systems. FL1-100% white LED lamp; FL2-100% blue LED lamp; FL3-100% red LED lamp; FL4-50% white + 25% red + 25% blue LED lamps; FL5-50% red + 50% blue LED lamps; FL6-25% red + 75% blue LED lamps; FL7-75% red + 25% blue LED lamps; FL8- white fluorescent lamp. CSS = conventional sealing system; SSGE = sealing system with gas exchange.

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Figure 4
Schomburgkia crispa plants at 180 days of ex vitro cultivation as a function of light sources and sealing systems. FL1-100% white LED lamp; FL2-100% blue LED lamp; FL3-100% red LED lamp; FL4-50% white + 25% red + 25% blue LED lamps; FL5-50% red + 50% blue LED lamps; FL6-25% red + 75% blue LED lamps; FL7-75% red + 25% blue LED lamps; FL8- white fluorescent lamp. CSS = conventional sealing system; SSGE = sealing system with gas exchange.

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Table 1
Plant height (PHe mm), length of largest leaf (LL mm), length of largest root (LR mm), total fresh mass (TFM) (g), and percentage of survival (%SUR) of Schomburgkia crispa in function of sealing system (CSS = conventional sealing system; SSGE = sealing system with gas exchange) and different light sources after four months of in vitro cultivation.

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Table 2
Increases (%) in plant height (PHe, mm), number of leaves (NL), number of roots (NR), length of largest leaf (LL mm), length of largest root (LR mm), total fresh mass (TFM), and percent survival (%SUR) of Schomburgkia crispa in function of sealing system (CSS = conventional sealing system; SSGE = sealing system with gas exchange) and different light sources after six months of ex vitro cultivation.

Flask	PHe		LL		LR		TFM		%SUR
Flask	CSS	SSGE	CSS	SSGE	CSS	SSGE	CSS	SSGE	CSS	SSGE
FL1	29.48 bB	40.88 bA	19.63 bA	28.46 bA	34.35 bA	44.82 aA	0.488 bB	0.999 aA	100.00 aA	100.00 aA
FL2	35.93 bA	38.64 bA	23.89 bA	28.83 bA	28.38 bA	30.84 bA	0.381 bA	0.586 bA	100.00 aA	100.00 aA
FL3	33.57 bB	40.67 bA	18.91 bB	28.06 bA	31.78 bA	31.48 bA	0.733 aA	0.584 bA	100.00 aA	100.00 aA
FL4	50.45 aA	30.12 bB	39.58 aA	20.40 bB	57.14 aA	32.13 bB	0.960 aA	0.431 bB	100.00 aA	100.00 aA
FL5	28.88 bB	42. 95 bA	19.73 bB	32.54 aA	27.93 bB	50.38 aA	0.404 bA	0.681 bA	100.00 aA	91.67 bB
FL6	34.62 bB	54.37 aA	21.83 bB	40.51 aA	39.02 bB	52.48 aA	0.630 bB	0.939 aA	100.00 aA	100.00 aA
FL7	35.70 bB	47.01 aA	22.23 bB	35.85 aA	37.17 bA	47.64 aA	0.491 bA	0.704 bA	100.00 aA	100.00 aA
FL8	28.83 bA	34.05 bA	18.03 bA	26.42 bA	33.77 bA	41.27 aA	0.498 bA	0.718 bA	100.00 aA	100.00 aA
GM	34.68	41.09	22.98	30.13	36.19	41.38	0.573	0.705	100.00	98.96
CV (%)	16.64		20.19		17.65		14.47		0.18

Flask	PHe		NL		NR		LL		LR		TFM		%SUR
Flask	CSS	SSGE	CSS	SSGE	CSS	SSGE	CSS	SSGE	CSS	SSGE	CSS	SSGE	CSS	SSGE
FL1	36.73 aA	27.77 cA	5.25 aA	6.17 aA	4.83 bA	5.83 aA	29.87 aA	22.42 cA	41.03 aA	27.90 bB	0.90 aA	0.73 bA	100.00 aA	100.00 aA
FL2	38.69 aA	2.28 dB	5.41 aA	0.17 bB	6.50 aA	0.33 cB	30.35 aA	0.70 dB	33.87 aA	1.67 dB	0.85 aA	0.02 cB	100.00 aA	8.33 cB
FL3	14.03 cB	43.96 bA	2.42 cB	5.25 aA	3.08 cB	3.74 aA	11.33 cB	38.90 bA	14.99 cB	35.22 aA	0.17 cB	0.64 bA	66.67 bB	100.00 aA
FL4	17.47 cB	62.48 aA	1.00 dB	6.08 aA	2.67 cB	5.25 aA	7.78 cB	49.74 aA	17.68 cB	45.23 aA	0.28 cB	1.11 aA	58.33 cB	100.00 aA
FL5	38.15 aB	49.12 bA	5.08 aA	4.25 aA	4.92 bA	4.92 aA	23.80 aB	34.00 bA	25.56 bB	38.72 aA	0.55 bA	0.75 bA	100.00 aA	100.00 aA
FL6	0.00 dB	39.43 bA	0.00 dB	5.42 aA	0.00 dB	5.83 aA	0.00 dB	36.32 bA	0.00 dB	39.21 aA	0.00 cB	1.06 aA	0.00 dB	100.00 aA
FL7	25.94 bA	30.61 cA	3.33 bA	1.75 bA	2.67 cA	2.25 bA	16.25 bA	19.81 cA	15.37 cA	17.71 cA	0.26 cA	0.19 cA	66.67 bB	83.33 bA
FL8	0.00 dB	28.10 cA	0.00 dB	5.50 aA	0.00 dB	4.50 aA	0.00 dB	23.89 cA	0.00 dB	28.58 bA	0.00 cB	0.54 bA	0.00 dB	100.00 aA
GM	21.4	35.47	2.81	4.32	3.08	4.08	14.92	28.22	18.56	29.28	0.38	0.63	61.46	86.46
CV (%)	26.38		22.28		22.20		27.90		29.79		11.01		1.08

[1] 7Author for correspondence: jessica_monico13@hotmail.com

Brasil

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In vitro and ex vitro production of Schomburgkia crispa: effect of flask sealing systems and different light sources

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