Filter paper performance in PCR for cutaneous leishmaniasis diagnosis

Abstract

INTRODUCTION:

The objective of this study was to evaluate the performance of filter paper (FP) for lesion scraping collection in a polymerase chain reaction (PCR) for cutaneous leishmaniasis (CL) diagnosis.

METHODS:

Lesion scrapings from 48 patients were collected and analyzed for PCR.

RESULTS:

PCR with FP detected up to three Leishmania braziliensis promastigotes. Considering the direct search by microscopy or PCR of samples collected in STE buffer as standards, the sensitivity of PCR with FP was 100%.

CONCLUSIONS:

FP can be useful for CL diagnosis in remote regions, allowing high sensitivity in the detection of the parasite by PCR.

Keywords:
Leishmania; Cutaneous leishmaniasis; Neglected diseases; Polymerase chain reaction; Diagnosis