Open-access Emprego de quatro exames imunológicos na determinação da prevalência da doença de chagas nos garis do serviço de limpeza urbana do distrito federal

Seropositivity for Trypanosoma cruzi infection was studied in 308 street-sweepers of the SLU. Federal District. Brazil, with the aid of haemaglutination. immunofluorescence and, also, a delayed-type skin test to the parasite T12E antigen. It showed 32,1%, 42.7% and 38.6% positive results, respectively for cach assay. Among these, however, only 47% were positive with each of three exams performed. In addition, 19.7% were positive with two out of three exams performed. The remaining 33-3% sera yielded one positive result out of three exams employed and were submitted to the immunoblot assay. This analysis confirmed 3 cases (37.5%) positive by hemmaglutination. 3 (11.5%) positive by skin test, and 1 (3-7%) positive by immunofluorescence. At the end of the analysis, it was shown that 129 (35%) individuals yielded at least two positive assays and therefore, they should be cousidered as T. cruzi-infected individuals.

Human T. cruzi infection; Serology; Delayed-tvpe skin hypersensitivity


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