<i>In vitro</i> antifungal activities of leaf
                    extracts of <i>Lippia alba</i> (Verbenaceae) against
                    clinically important yeast species

Oliveira, Graziela Teixeira de; Ferreira, Jaqueline Maria Siqueira; Rosa, Luiz Henrique; Siqueira, Ezequias Pessoa de; Johann, Susana; Lima, Luciana Alves Rodrigues dos Santos

doi:10.1590/0037-8682-0008-2013

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Revista da Sociedade Brasileira de Medicina Tropical

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Short Communications • Rev. Soc. Bras. Med. Trop. 47 (2) • Mar-Apr 2014 • https://doi.org/10.1590/0037-8682-0008-2013 copy

In vitro antifungal activities of leaf extracts of Lippia alba (Verbenaceae) against clinically important yeast species

Authorship SCIMAGO INSTITUTIONS RANKINGS

Introduction

There are few studies reporting the antifungal activities of Lippia alba extracts.

Methods

A broth microdilution assay was used to evaluate the antifungal effects of Lippia alba extracts against seven yeast species of Candida and Cryptococcus. The butanol fraction was investigated by gas chromatography-mass spectrometry.

Results

The butanol fraction showed the highest activity against Candida glabrata. The fraction also acted synergistically with itraconazole and fluconazole against C. glabrata. The dominant compounds in the butanol fraction were 2,2,5-trimethyl-3,4-hexanedione, 3,5-dimethyl-4-octanone and hexadecane.

Conclusions

The butanol fraction may be a good candidate in the search for new drugs from natural products with antifungal activity.

Lippia alba ; Antifungal activity; Yeast

Fungi	MIC (µg/mL)
Fungi	Et	Hex	DCM	Ac	But	HE	amphotericin B	itraconazole	fluconazole
Candida albicans	2,000	≥ 2,000	≥ 2,000	≥ 2,000	2,000	2,000	0.25	0.031	2
Candida glabrata	2,000	≥ 2,000	≥ 2,000	≥ 2,000	62.5	2,000	0.125	0.25	4
Candida krusei	1,000	≥ 2,000	2,000	1,000	2,000	2,000	0.5	0.125	32
Candida parapsilosis	1,000	≥ 2,000	≥ 2,000	≥ 2,000	≥ 2,000	2,000	0.5	0.062	1
Candida tropicalis	2,000	≥ 2,000	≥ 2,000	≥ 2,000	≥ 2,000	≥ 2,000	1.0	0.062	2
Cryptococcus gattii	≥ 2,000	≥ 2,000	≥ 2,000	≥ 2,000	2,000	2,000	1.0	0.031	nt
Cryptococcus neoformans	1,000	≥ 2,000	≥ 2,000	≥ 2,000	2,000	2,000	1.0	0.031	nt

Samples	MIC in combination (µg/mL)	MIC alone (µg/mL)	FIC	FICI
1. Butanol fraction	0.975	62.5	0.015	1.015
2. Amphotericin B	0.125	0.125	1
1. Butanol fraction	0.975	62.5	0.015	0.075
2. Itraconazole	0.015	0.25	0.06
1. Butanol fraction	0.975	62.5	0.0156	0.031
2. Fluconazole	0.062	4	0.0155

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[1] CONFLICT OF INTEREST The authors declare that there is no conflict of interest.