Viability and molecular authentication of Coccidioides immitis strains from culture collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil

Viabilidade e autenticação molecular de cepas de Coccidioides immitis da coleção de culturas do Instituto Oswaldo Cruz, Rio de Janeiro, Brasil

Claudia C.F. Bezerra Renata F. de Lima Márcia S. Lazera Bodo Wanke Cíntia M. Borba About the authors

Twenty Coccidioides immitis strains were evaluated. Only 5 of the 20 strains kept under mineral oil maintained their viability while all 5 subcultures preserved in water remained viable and none of the 13 subcultures kept in soil were viable. A 519 bp PCR product from the csa gene confirmed the identity of the strains.

Coccidioides immitis; Long-term preservation; PCR


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