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Articles • Rev. Soc. Bras. Med. Trop. 39 (3) • June 2006 • https://doi.org/10.1590/S0037-86822006000300002   copy

Viability and molecular authentication of Coccidioides immitis strains from culture collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil

Viabilidade e autenticação molecular de cepas de Coccidioides immitis da coleção de culturas do Instituto Oswaldo Cruz, Rio de Janeiro, Brasil

Authorship SCIMAGO INSTITUTIONS RANKINGS

Twenty Coccidioides immitis strains were evaluated. Only 5 of the 20 strains kept under mineral oil maintained their viability while all 5 subcultures preserved in water remained viable and none of the 13 subcultures kept in soil were viable. A 519 bp PCR product from the csa gene confirmed the identity of the strains.

Coccidioides immitis; Long-term preservation; PCR

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