The authors have developed a process to make hemocultures in chronic phase of human Chagas' disease. They used as inocule the washed packed cells of 10 ml of blood. This material was sampled in 2 tubes of LIT (Liver Infusion Tryptose) with 5 ml of the medium. Six positive cases (30%) were detected with only one hemoculture. Increasing the nurriber of hemocultures (1 to 14) 9 out of 20 cases were positives (45%). In 7 cases 4 or more hemocultures (4, 5, 6, 10, 14) were made and 5 of them were positives (71,4%). The authors recomend the method for routine as a practical and easy method