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Utilização do picro-sirius para corar o Paracoccidioides brasiliensis

Picrosirius stain was used for Paracoccidioides brasiliensis in formalin-fixed human tissue. By common light microscopy the fungus shows a red peripheral band and on polarization microscopy, one or more birefringent green rings. Previous immersion in 1% sodium hidroxide solution enhances the staining and the birefringency of parasitic cells. The differentiation of the stained sections with 0,5% sodium hydroxide eliminates the birefringency of the collagen more rapidly than that of the paraeoccidioides.

Paracoccidioides brasiliensis; Paracoccidiodomycosis; Picrosirius; Histotechnology; Mycosis

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